Genome-Wide Identification and Analysis of the <i>ABCF</i> Gene Family in <i>Triticum aestivum</i>
The ATP-binding cassette (ABC) superfamily of proteins is a group of evolutionarily conserved proteins. The <i>ABCF</i> subfamily is involved in ribosomal synthesis, antibiotic resistance, and transcriptional regulation. However, few studies have investigated the role of <i>ABCF<...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/22/16478 |
| Summary: | The ATP-binding cassette (ABC) superfamily of proteins is a group of evolutionarily conserved proteins. The <i>ABCF</i> subfamily is involved in ribosomal synthesis, antibiotic resistance, and transcriptional regulation. However, few studies have investigated the role of <i>ABCF</i> in wheat (<i>Triticum aestivum</i>) immunity. Here, we identified 18 <i>TaABCF</i>s and classified them into four categories based on their domain characteristics. Functional similarity between <i>Arabidopsis</i> and wheat <i>ABCF</i> genes was predicted using phylogenetic analysis. A comprehensive genome-wide analysis of gene structure, protein motifs, chromosomal location, and cis-acting elements was also performed. Tissue-specific analysis and expression profiling under temperature, hormonal, and viral stresses were performed using real-time quantitative reverse transcription polymerase chain reaction after randomly selecting one gene from each group. The results revealed that all <i>TaABCF</i> genes had the highest expression at 25 °C and responded to methyl jasmonate induction. Notably, <i>TaABCF2</i> was highly expressed in all tissues except the roots, and silencing it significantly increased the accumulation of Chinese wheat mosaic virus or wheat yellow mosaic virus in wheat leaves. These results indicated that <i>TaABCF</i> may function in response to viral infection, laying the foundation for further studies on the mechanisms of this protein family in plant defence. |
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| ISSN: | 1661-6596 1422-0067 |