Rapid detection of <it>Pseudomonas aeruginosa </it>from positive blood cultures by quantitative PCR
<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas aeruginosa </it>is responsible for numerous bloodstream infections associated with severe adverse outcomes in case of inappropriate initial antimicrobial therapy. The present study was aimed to develop a n...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-08-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Online Access: | http://www.ann-clinmicrob.com/content/9/1/21 |
| _version_ | 1828434120466759680 |
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| author | Cattoir Vincent Gilibert Audrey Le Glaunec Jeanne-Marie Launay Nathalie Bait-Mérabet Lilia Legrand Patrick |
| author_facet | Cattoir Vincent Gilibert Audrey Le Glaunec Jeanne-Marie Launay Nathalie Bait-Mérabet Lilia Legrand Patrick |
| author_sort | Cattoir Vincent |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Pseudomonas aeruginosa </it>is responsible for numerous bloodstream infections associated with severe adverse outcomes in case of inappropriate initial antimicrobial therapy. The present study was aimed to develop a novel quantitative PCR (qPCR) assay, using <it>ecfX </it>as the specific target gene, for the rapid and accurate identification of <it>P. aeruginosa </it>from positive blood cultures (BCs).</p> <p>Methods</p> <p>Over the period August 2008 to June 2009, 100 BC bottles positive for gram-negative bacilli were tested in order to evaluate performances of the qPCR technique with conventional methods as gold standard (i.e. culture and phenotypic identification).</p> <p>Results</p> <p>Thirty-three strains of <it>P. aeruginosa</it>, 53 strains of Enterobactericaeae, nine strains of <it>Stenotrophomonas maltophilia </it>and two other gram-negative species were isolated while 3 BCs were polymicrobial including one mixture containing <it>P. aeruginosa</it>. All <it>P. aeruginosa </it>clinical isolates were detected by qPCR except a single strain in mixed culture. Performances of the qPCR technique were: specificity, 100%; positive predictive value, 100%; negative predictive value, 98.5%; and sensitivity, 97%.</p> <p>Conclusions</p> <p>This reliable technique may offer a rapid (<1.5 h) tool that would help clinicians to initiate an appropriate treatment earlier. Further investigations are needed to assess the clinical benefit of this novel strategy as compared to phenotypic methods.</p> |
| first_indexed | 2024-12-10T18:45:46Z |
| format | Article |
| id | doaj.art-03f0494ef7b8404cba5abab473d8cbaa |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-12-10T18:45:46Z |
| publishDate | 2010-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-03f0494ef7b8404cba5abab473d8cbaa2022-12-22T01:37:30ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112010-08-01912110.1186/1476-0711-9-21Rapid detection of <it>Pseudomonas aeruginosa </it>from positive blood cultures by quantitative PCRCattoir VincentGilibert AudreyLe Glaunec Jeanne-MarieLaunay NathalieBait-Mérabet LiliaLegrand Patrick<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas aeruginosa </it>is responsible for numerous bloodstream infections associated with severe adverse outcomes in case of inappropriate initial antimicrobial therapy. The present study was aimed to develop a novel quantitative PCR (qPCR) assay, using <it>ecfX </it>as the specific target gene, for the rapid and accurate identification of <it>P. aeruginosa </it>from positive blood cultures (BCs).</p> <p>Methods</p> <p>Over the period August 2008 to June 2009, 100 BC bottles positive for gram-negative bacilli were tested in order to evaluate performances of the qPCR technique with conventional methods as gold standard (i.e. culture and phenotypic identification).</p> <p>Results</p> <p>Thirty-three strains of <it>P. aeruginosa</it>, 53 strains of Enterobactericaeae, nine strains of <it>Stenotrophomonas maltophilia </it>and two other gram-negative species were isolated while 3 BCs were polymicrobial including one mixture containing <it>P. aeruginosa</it>. All <it>P. aeruginosa </it>clinical isolates were detected by qPCR except a single strain in mixed culture. Performances of the qPCR technique were: specificity, 100%; positive predictive value, 100%; negative predictive value, 98.5%; and sensitivity, 97%.</p> <p>Conclusions</p> <p>This reliable technique may offer a rapid (<1.5 h) tool that would help clinicians to initiate an appropriate treatment earlier. Further investigations are needed to assess the clinical benefit of this novel strategy as compared to phenotypic methods.</p>http://www.ann-clinmicrob.com/content/9/1/21 |
| spellingShingle | Cattoir Vincent Gilibert Audrey Le Glaunec Jeanne-Marie Launay Nathalie Bait-Mérabet Lilia Legrand Patrick Rapid detection of <it>Pseudomonas aeruginosa </it>from positive blood cultures by quantitative PCR Annals of Clinical Microbiology and Antimicrobials |
| title | Rapid detection of <it>Pseudomonas aeruginosa </it>from positive blood cultures by quantitative PCR |
| title_full | Rapid detection of <it>Pseudomonas aeruginosa </it>from positive blood cultures by quantitative PCR |
| title_fullStr | Rapid detection of <it>Pseudomonas aeruginosa </it>from positive blood cultures by quantitative PCR |
| title_full_unstemmed | Rapid detection of <it>Pseudomonas aeruginosa </it>from positive blood cultures by quantitative PCR |
| title_short | Rapid detection of <it>Pseudomonas aeruginosa </it>from positive blood cultures by quantitative PCR |
| title_sort | rapid detection of it pseudomonas aeruginosa it from positive blood cultures by quantitative pcr |
| url | http://www.ann-clinmicrob.com/content/9/1/21 |
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