Transcriptomic analysis reveals mechanisms for the different drought tolerance of sweet potatoes
Drought is a common environmental stress with great negative impacts on plant growth, development and geographical distribution as well as agriculture and food production. Sweet potato is characterized by starchy, fresh and pigmented tuber, and is regarded as the seventh most important food crop. Ho...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1136709/full |
| _version_ | 1827989099159486464 |
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| author | Enliang Liu Linli Xu Zhengqian Luo Zhiqiang Li Guohui Zhou Haifeng Gao Furong Fang Jun Tang Yue Zhao Zhilin Zhou Ping Jin |
| author_facet | Enliang Liu Linli Xu Zhengqian Luo Zhiqiang Li Guohui Zhou Haifeng Gao Furong Fang Jun Tang Yue Zhao Zhilin Zhou Ping Jin |
| author_sort | Enliang Liu |
| collection | DOAJ |
| description | Drought is a common environmental stress with great negative impacts on plant growth, development and geographical distribution as well as agriculture and food production. Sweet potato is characterized by starchy, fresh and pigmented tuber, and is regarded as the seventh most important food crop. However, there has been no comprehensive study of the drought tolerance mechanism of different sweet potato cultivars to date. Here, we studied the mechanism for drought response of seven sweet potato drought-tolerant cultivars using the drought coefficients, physiological indicators and transcriptome sequencing. The seven sweet potato cultivars were classified into four groups of drought tolerance performance. A large number of new genes and transcripts were identified, with an average of about 8000 new genes per sample. Alternative splicing events in sweet potato, which were dominated by first exon and last exon alternative splicing, were not conserved among different cultivars and not significantly affected by drought stress. Furthermore, different drought-tolerance mechanisms were revealed through differentially expressed gene analysis and functional annotation. Two drought-sensitive cultivars, Shangshu-9 and Xushu-22, mainly resisted drought stress by up-regulating plant signal transduction. The other drought-sensitive cultivar Jishu-26 responded to drought stress by down-regulating isoquinoline alkaloid biosynthesis and nitrogen/carbohydrate metabolism. In addition, the drought-tolerant cultivar Chaoshu-1 and drought-preferred cultivar Z15-1 only shared 9% of differentially expressed genes, as well as many opposite metabolic pathways in response to drought. They mainly regulated flavonoid and carbohydrate biosynthesis/metabolism in response to drought, while Z15-1 increased photosynthesis and carbon fixation capacity. The other drought-tolerant cultivar Xushu-18 responded to drought stress by regulating the isoquinoline alkaloid biosynthesis and nitrogen/carbohydrate metabolism. The extremely drought-tolerant cultivar Xuzi-8 was almost unaffected by drought stress and responded to drought environment only by regulating the cell wall. These findings provide important information for the selection of sweet potatoes for specific purposes. |
| first_indexed | 2024-04-10T00:13:59Z |
| format | Article |
| id | doaj.art-03f0fd73d3724f3d8ad7a47fe3c533b3 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T00:13:59Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-03f0fd73d3724f3d8ad7a47fe3c533b32023-03-16T06:09:32ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.11367091136709Transcriptomic analysis reveals mechanisms for the different drought tolerance of sweet potatoesEnliang Liu0Linli Xu1Zhengqian Luo2Zhiqiang Li3Guohui Zhou4Haifeng Gao5Furong Fang6Jun Tang7Yue Zhao8Zhilin Zhou9Ping Jin10Grain Crops Institute, Xinjiang Academy of Agricultural Sciences, Urumqi, ChinaComprehensive Proving Ground, Xinjiang Academy of Agricultural Sciences, Urumqi, ChinaComprehensive Proving Ground, Xinjiang Academy of Agricultural Sciences, Urumqi, ChinaGrain Crops Institute, Xinjiang Academy of Agricultural Sciences, Urumqi, ChinaAdsen Biotechnology Co., Ltd., Urumqi, ChinaInstitute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi, ChinaGrain Crops Institute, Xinjiang Academy of Agricultural Sciences, Urumqi, ChinaXuzhou Institute of Agricultural Sciences in Xuhuai District, Xuzhou, ChinaGrain Crops Institute, Xinjiang Academy of Agricultural Sciences, Urumqi, ChinaXuzhou Institute of Agricultural Sciences in Xuhuai District, Xuzhou, ChinaComprehensive Proving Ground, Xinjiang Academy of Agricultural Sciences, Urumqi, ChinaDrought is a common environmental stress with great negative impacts on plant growth, development and geographical distribution as well as agriculture and food production. Sweet potato is characterized by starchy, fresh and pigmented tuber, and is regarded as the seventh most important food crop. However, there has been no comprehensive study of the drought tolerance mechanism of different sweet potato cultivars to date. Here, we studied the mechanism for drought response of seven sweet potato drought-tolerant cultivars using the drought coefficients, physiological indicators and transcriptome sequencing. The seven sweet potato cultivars were classified into four groups of drought tolerance performance. A large number of new genes and transcripts were identified, with an average of about 8000 new genes per sample. Alternative splicing events in sweet potato, which were dominated by first exon and last exon alternative splicing, were not conserved among different cultivars and not significantly affected by drought stress. Furthermore, different drought-tolerance mechanisms were revealed through differentially expressed gene analysis and functional annotation. Two drought-sensitive cultivars, Shangshu-9 and Xushu-22, mainly resisted drought stress by up-regulating plant signal transduction. The other drought-sensitive cultivar Jishu-26 responded to drought stress by down-regulating isoquinoline alkaloid biosynthesis and nitrogen/carbohydrate metabolism. In addition, the drought-tolerant cultivar Chaoshu-1 and drought-preferred cultivar Z15-1 only shared 9% of differentially expressed genes, as well as many opposite metabolic pathways in response to drought. They mainly regulated flavonoid and carbohydrate biosynthesis/metabolism in response to drought, while Z15-1 increased photosynthesis and carbon fixation capacity. The other drought-tolerant cultivar Xushu-18 responded to drought stress by regulating the isoquinoline alkaloid biosynthesis and nitrogen/carbohydrate metabolism. The extremely drought-tolerant cultivar Xuzi-8 was almost unaffected by drought stress and responded to drought environment only by regulating the cell wall. These findings provide important information for the selection of sweet potatoes for specific purposes.https://www.frontiersin.org/articles/10.3389/fpls.2023.1136709/fullsweet potatodrought stresstranscriptomicsdifferentially expressed genesdrought tolerance |
| spellingShingle | Enliang Liu Linli Xu Zhengqian Luo Zhiqiang Li Guohui Zhou Haifeng Gao Furong Fang Jun Tang Yue Zhao Zhilin Zhou Ping Jin Transcriptomic analysis reveals mechanisms for the different drought tolerance of sweet potatoes Frontiers in Plant Science sweet potato drought stress transcriptomics differentially expressed genes drought tolerance |
| title | Transcriptomic analysis reveals mechanisms for the different drought tolerance of sweet potatoes |
| title_full | Transcriptomic analysis reveals mechanisms for the different drought tolerance of sweet potatoes |
| title_fullStr | Transcriptomic analysis reveals mechanisms for the different drought tolerance of sweet potatoes |
| title_full_unstemmed | Transcriptomic analysis reveals mechanisms for the different drought tolerance of sweet potatoes |
| title_short | Transcriptomic analysis reveals mechanisms for the different drought tolerance of sweet potatoes |
| title_sort | transcriptomic analysis reveals mechanisms for the different drought tolerance of sweet potatoes |
| topic | sweet potato drought stress transcriptomics differentially expressed genes drought tolerance |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1136709/full |
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