COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL
This study aimed to determine whether the variant or biotype of Trypanosoma evansi can be seen from their polypeptide profiles using 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) stained with Brilliant Blue Commasie. The results generally showed that the molecular weight (...
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| Format: | Article |
| Language: | English |
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Universitas Syiah Kuala
2018-10-01
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| Series: | Jurnal Kedokteran Hewan |
| Subjects: | |
| Online Access: | https://jurnal.usk.ac.id/JKH/article/view/11486 |
| _version_ | 1827266096670965760 |
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| author | Ichwan Yuniarto Didik T Subekti Umi Cahyaningsih Fadjar Satrija |
| author_facet | Ichwan Yuniarto Didik T Subekti Umi Cahyaningsih Fadjar Satrija |
| author_sort | Ichwan Yuniarto |
| collection | DOAJ |
| description | This study aimed to determine whether the variant or biotype of Trypanosoma evansi can be seen from their polypeptide profiles using 12%
sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) stained with Brilliant Blue Commasie. The results generally showed that
the molecular weight (MW) of polypeptides from nine isolates from East Java, Central Java, Banten, South Kalimantan, Central Kalimantan, and
Lampung provinces were in the range of 85.46 to 15.76 kD and each isolate has different polypeptide profile. Isolates A13 and A14 were isolated
from the same place but have different polypeptide profiles. Likewise, isolates S13 and S18 also have different polypeptide profiles despite being
isolated from the same place at the same time. On the other hand, isolate 372, 87, and 06 have different protein profiles but was classified in the
same biotype namely biotype I. Generally, the difference in protein profile actually more related to the biological diversity of the metabolism of
each Trypanosoma evansi isolate from Indonesia. |
| first_indexed | 2024-03-07T16:16:28Z |
| format | Article |
| id | doaj.art-03f2304cb8e5442e8229949900769e4b |
| institution | Directory Open Access Journal |
| issn | 1978-225X 2502-5600 |
| language | English |
| last_indexed | 2025-03-22T04:12:25Z |
| publishDate | 2018-10-01 |
| publisher | Universitas Syiah Kuala |
| record_format | Article |
| series | Jurnal Kedokteran Hewan |
| spelling | doaj.art-03f2304cb8e5442e8229949900769e4b2024-04-28T08:37:50ZengUniversitas Syiah KualaJurnal Kedokteran Hewan1978-225X2502-56002018-10-0112210.21157/j.ked.hewan.v12i2.114868957COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDALIchwan Yuniarto0Didik T Subekti1Umi Cahyaningsih2Fadjar Satrija3Veterinary Center of Banjarbaru, Banjarbaru, IndonesiaIndonesian Research Center of Veterinary Science, Bogor, IndonesiaFaculty of Veterinary Health, Bogor Agriculture Institute, Bogor, IndonesiaFaculty of Veterinary Health, Bogor Agriculture Institute, Bogor, IndonesiaThis study aimed to determine whether the variant or biotype of Trypanosoma evansi can be seen from their polypeptide profiles using 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) stained with Brilliant Blue Commasie. The results generally showed that the molecular weight (MW) of polypeptides from nine isolates from East Java, Central Java, Banten, South Kalimantan, Central Kalimantan, and Lampung provinces were in the range of 85.46 to 15.76 kD and each isolate has different polypeptide profile. Isolates A13 and A14 were isolated from the same place but have different polypeptide profiles. Likewise, isolates S13 and S18 also have different polypeptide profiles despite being isolated from the same place at the same time. On the other hand, isolate 372, 87, and 06 have different protein profiles but was classified in the same biotype namely biotype I. Generally, the difference in protein profile actually more related to the biological diversity of the metabolism of each Trypanosoma evansi isolate from Indonesia.https://jurnal.usk.ac.id/JKH/article/view/11486biotypeprotein profilesds pagesurratrypanosoma evansi |
| spellingShingle | Ichwan Yuniarto Didik T Subekti Umi Cahyaningsih Fadjar Satrija COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL Jurnal Kedokteran Hewan biotype protein profile sds page surra trypanosoma evansi |
| title | COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL |
| title_full | COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL |
| title_fullStr | COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL |
| title_full_unstemmed | COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL |
| title_short | COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL |
| title_sort | comparison of polypeptide profile of trypanosoma evansi isolates from indonesia and their relation to biotype and sensitivity to trypanocidal |
| topic | biotype protein profile sds page surra trypanosoma evansi |
| url | https://jurnal.usk.ac.id/JKH/article/view/11486 |
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