Batch Fermentation of Salt-Acclimatizing Microalga for Omega-3 Docosahexaenoic Acid Production Using Biodiesel-Derived Crude Glycerol Waste as a Low-Cost Substrate
Biodiesel produced from waste cooking oil (WCO) is on the rise and inevitably leads to issues in managing glycerol waste. Due to the presence of colour, odour and other minor compounds, the refining costs for this type of glycerol are higher and uneconomical. The potential of biodiesel-derived glyce...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/10/2/86 |
| _version_ | 1797298252296486912 |
|---|---|
| author | Varavut Tanamool Prayoon Enmak Pakawadee Kaewkannetra |
| author_facet | Varavut Tanamool Prayoon Enmak Pakawadee Kaewkannetra |
| author_sort | Varavut Tanamool |
| collection | DOAJ |
| description | Biodiesel produced from waste cooking oil (WCO) is on the rise and inevitably leads to issues in managing glycerol waste. Due to the presence of colour, odour and other minor compounds, the refining costs for this type of glycerol are higher and uneconomical. The potential of biodiesel-derived glycerol waste (BDGW) obtained from WCO to produce the highly added product of docosahexaenoic acid (DHA), also known as omega-3 polyunsaturated fatty acid, via the marine microalga of <i>Schizochytrium limacinum</i> ATCC MYA-1381 under aerobic batch fermentation was investigated. Cell growth, as well as DHA production, were performed under various operating conditions, including aeration rates and BDGW concentrations. The effect of the substrate type on cell growth and DHA yield was evaluated. The optimum operating condition was obtained when the air flow of a 0.25 vvm and 50 g/L of the glycerol concentration was fed into the fermenter and maximum cell dry weight (11.40 g/L) and DHA yield (665.52 mg/g) were achieved. However, cell growth and DHA yield were not significantly different when <i>S. limacinum</i> was grown using various carbon sources. Successfully, it clearly demonstrates that the BDGW can be used as a cheap carbon source for DHA production via marine microalgae using aerobic batch fermentation. |
| first_indexed | 2024-03-07T22:33:11Z |
| format | Article |
| id | doaj.art-03fa21bee29b43adbd9ee5bbf56f8f01 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-07T22:33:11Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-03fa21bee29b43adbd9ee5bbf56f8f012024-02-23T15:15:54ZengMDPI AGFermentation2311-56372024-01-011028610.3390/fermentation10020086Batch Fermentation of Salt-Acclimatizing Microalga for Omega-3 Docosahexaenoic Acid Production Using Biodiesel-Derived Crude Glycerol Waste as a Low-Cost SubstrateVaravut Tanamool0Prayoon Enmak1Pakawadee Kaewkannetra2Chemistry Program, Faculty of Science and Technology, Nakhon Ratchasima Rajabhat University, Nakhon Ratchasima 30000, ThailandSchool of Chemical Engineering and Advanced Materials, Newcastle University, Merz Court, Newcastle upon Tyne NE1 7RU, UKDepartment of Biotechnology, Faculty of Technology, Khon Kaen Univerisity, Khon Kaen 40002, ThailandBiodiesel produced from waste cooking oil (WCO) is on the rise and inevitably leads to issues in managing glycerol waste. Due to the presence of colour, odour and other minor compounds, the refining costs for this type of glycerol are higher and uneconomical. The potential of biodiesel-derived glycerol waste (BDGW) obtained from WCO to produce the highly added product of docosahexaenoic acid (DHA), also known as omega-3 polyunsaturated fatty acid, via the marine microalga of <i>Schizochytrium limacinum</i> ATCC MYA-1381 under aerobic batch fermentation was investigated. Cell growth, as well as DHA production, were performed under various operating conditions, including aeration rates and BDGW concentrations. The effect of the substrate type on cell growth and DHA yield was evaluated. The optimum operating condition was obtained when the air flow of a 0.25 vvm and 50 g/L of the glycerol concentration was fed into the fermenter and maximum cell dry weight (11.40 g/L) and DHA yield (665.52 mg/g) were achieved. However, cell growth and DHA yield were not significantly different when <i>S. limacinum</i> was grown using various carbon sources. Successfully, it clearly demonstrates that the BDGW can be used as a cheap carbon source for DHA production via marine microalgae using aerobic batch fermentation.https://www.mdpi.com/2311-5637/10/2/86biodieselglycerol wastewaste cooking oil (WCO)biodieseldocosahexaenoic acid (DHA)<i>Schizochytrium limacinum</i> |
| spellingShingle | Varavut Tanamool Prayoon Enmak Pakawadee Kaewkannetra Batch Fermentation of Salt-Acclimatizing Microalga for Omega-3 Docosahexaenoic Acid Production Using Biodiesel-Derived Crude Glycerol Waste as a Low-Cost Substrate Fermentation biodiesel glycerol waste waste cooking oil (WCO) biodiesel docosahexaenoic acid (DHA) <i>Schizochytrium limacinum</i> |
| title | Batch Fermentation of Salt-Acclimatizing Microalga for Omega-3 Docosahexaenoic Acid Production Using Biodiesel-Derived Crude Glycerol Waste as a Low-Cost Substrate |
| title_full | Batch Fermentation of Salt-Acclimatizing Microalga for Omega-3 Docosahexaenoic Acid Production Using Biodiesel-Derived Crude Glycerol Waste as a Low-Cost Substrate |
| title_fullStr | Batch Fermentation of Salt-Acclimatizing Microalga for Omega-3 Docosahexaenoic Acid Production Using Biodiesel-Derived Crude Glycerol Waste as a Low-Cost Substrate |
| title_full_unstemmed | Batch Fermentation of Salt-Acclimatizing Microalga for Omega-3 Docosahexaenoic Acid Production Using Biodiesel-Derived Crude Glycerol Waste as a Low-Cost Substrate |
| title_short | Batch Fermentation of Salt-Acclimatizing Microalga for Omega-3 Docosahexaenoic Acid Production Using Biodiesel-Derived Crude Glycerol Waste as a Low-Cost Substrate |
| title_sort | batch fermentation of salt acclimatizing microalga for omega 3 docosahexaenoic acid production using biodiesel derived crude glycerol waste as a low cost substrate |
| topic | biodiesel glycerol waste waste cooking oil (WCO) biodiesel docosahexaenoic acid (DHA) <i>Schizochytrium limacinum</i> |
| url | https://www.mdpi.com/2311-5637/10/2/86 |
| work_keys_str_mv | AT varavuttanamool batchfermentationofsaltacclimatizingmicroalgaforomega3docosahexaenoicacidproductionusingbiodieselderivedcrudeglycerolwasteasalowcostsubstrate AT prayoonenmak batchfermentationofsaltacclimatizingmicroalgaforomega3docosahexaenoicacidproductionusingbiodieselderivedcrudeglycerolwasteasalowcostsubstrate AT pakawadeekaewkannetra batchfermentationofsaltacclimatizingmicroalgaforomega3docosahexaenoicacidproductionusingbiodieselderivedcrudeglycerolwasteasalowcostsubstrate |