Exploring the mechanism of Semen Cuscutae processed with salt solution in improving kidney deficiency miscarriage based on serum pharmacochemistry and network pharmacology
Semen Cuscutae (S) is an effective traditional Chinese medicine (TCM) in clinical practice for tonifying the kidney and calming the fetus. The effects of S on benefiting the kidney, fixing essence and calming the fetus were enhanced after salt processed. However, its bioactive substance and relevant...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223009188 |
| _version_ | 1797390456101797888 |
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| author | Xue Zhang Yu Huang Zhitong Yang Baiyang Xu Zilu Liu Ximeng Ding Qiumei Zhou Gang Cao Weidong Li Chuanshan Jin Shanshan Li Xiaoli Wang Jijun Chu |
| author_facet | Xue Zhang Yu Huang Zhitong Yang Baiyang Xu Zilu Liu Ximeng Ding Qiumei Zhou Gang Cao Weidong Li Chuanshan Jin Shanshan Li Xiaoli Wang Jijun Chu |
| author_sort | Xue Zhang |
| collection | DOAJ |
| description | Semen Cuscutae (S) is an effective traditional Chinese medicine (TCM) in clinical practice for tonifying the kidney and calming the fetus. The effects of S on benefiting the kidney, fixing essence and calming the fetus were enhanced after salt processed. However, its bioactive substance and relevant mechanism have not been clearly yet. This study aims to explore the effective components of Semen Cuscutae processed with salt solution (Y) and its potential mechanism on the treatment of kidney deficiency miscarriage (KDM) with multi-components and multi-targets. In this study, serum pharmacochemistry and network pharmacology were used to investigate the active ingredients and molecular mechanism of Y in improving kidney deficiency abortion. A rat model of kidney deficiency miscarriage was established using hydroxyurea combine with mifepristone to assess the effect of S on tonifying the kidney and calming the fetus. The results show that 13 active ingredients derived from Y were identified by serum pharmacochemistry analysis. 92 common targets of active ingredients of Y and diseases were screened by network pharmacology, including 18 key targets (TP53, AKT1, TNF, IL6, VEGFA, CASP3, JUN, EGFR, etc). The KEGG pathway enrichment analysis showed that it was mainly concentrated in PI3K/Akt signaling pathway, and it was speculated that Y may protect pregnancy through PI3K/AKT and other signaling pathways with multi-component, multi-target and multi-pathway action characteristics. Animal pharmacodynamic analysis suggested that Y showed the better effect on the hydroxyurea combine with mifepristone induced abortion compared to the raw products of S. This study laid the foundation to identify the active components and potential mechanisms of Y in improving KDM by combining the in-tegrated methods of serum pharmacochemistry and network pharmacology. |
| first_indexed | 2024-03-08T23:12:06Z |
| format | Article |
| id | doaj.art-03fa50c5e8084bf9b512258a49f1716f |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-08T23:12:06Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-03fa50c5e8084bf9b512258a49f1716f2023-12-15T07:23:35ZengElsevierArabian Journal of Chemistry1878-53522024-01-01171105456Exploring the mechanism of Semen Cuscutae processed with salt solution in improving kidney deficiency miscarriage based on serum pharmacochemistry and network pharmacologyXue Zhang0Yu Huang1Zhitong Yang2Baiyang Xu3Zilu Liu4Ximeng Ding5Qiumei Zhou6Gang Cao7Weidong Li8Chuanshan Jin9Shanshan Li10Xiaoli Wang11Jijun Chu12Anhui Province Key Laboratory of Research ＆ Development of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, ChinaAnhui Province Key Laboratory of Research ＆ Development of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, ChinaAnhui Province Key Laboratory of Research ＆ Development of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, ChinaAnhui Province Key Laboratory of Research ＆ Development of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, ChinaAnhui Province Key Laboratory of Research ＆ Development of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, ChinaAnhui Province Key Laboratory of Research ＆ Development of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, ChinaThe First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, ChinaSchool of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaAnhui Province Key Laboratory of Research ＆ Development of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, China; Heritage Base of TCM Processing Technology of NATCM, Anhui University of Chinese Medicine, Hefei 230012, China; Anhui Province Key Laboratory of Traditional Chinese Medicine Decoction Pieces of New Manufacturing Technology, Bozhou 236800, ChinaSchool of Pharmacy, Bengbu Medical College, Bengbu 233000, ChinaAnhui Province Key Laboratory of Research ＆ Development of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, China; Heritage Base of TCM Processing Technology of NATCM, Anhui University of Chinese Medicine, Hefei 230012, China; Anhui Province Key Laboratory of Traditional Chinese Medicine Decoction Pieces of New Manufacturing Technology, Bozhou 236800, China; Corresponding authors at: College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012(X. Wang), The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031(J. Chu).Department of Reproductive Medicine, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, China; Corresponding authors at: College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012(X. Wang), The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031(J. Chu).Semen Cuscutae (S) is an effective traditional Chinese medicine (TCM) in clinical practice for tonifying the kidney and calming the fetus. The effects of S on benefiting the kidney, fixing essence and calming the fetus were enhanced after salt processed. However, its bioactive substance and relevant mechanism have not been clearly yet. This study aims to explore the effective components of Semen Cuscutae processed with salt solution (Y) and its potential mechanism on the treatment of kidney deficiency miscarriage (KDM) with multi-components and multi-targets. In this study, serum pharmacochemistry and network pharmacology were used to investigate the active ingredients and molecular mechanism of Y in improving kidney deficiency abortion. A rat model of kidney deficiency miscarriage was established using hydroxyurea combine with mifepristone to assess the effect of S on tonifying the kidney and calming the fetus. The results show that 13 active ingredients derived from Y were identified by serum pharmacochemistry analysis. 92 common targets of active ingredients of Y and diseases were screened by network pharmacology, including 18 key targets (TP53, AKT1, TNF, IL6, VEGFA, CASP3, JUN, EGFR, etc). The KEGG pathway enrichment analysis showed that it was mainly concentrated in PI3K/Akt signaling pathway, and it was speculated that Y may protect pregnancy through PI3K/AKT and other signaling pathways with multi-component, multi-target and multi-pathway action characteristics. Animal pharmacodynamic analysis suggested that Y showed the better effect on the hydroxyurea combine with mifepristone induced abortion compared to the raw products of S. This study laid the foundation to identify the active components and potential mechanisms of Y in improving KDM by combining the in-tegrated methods of serum pharmacochemistry and network pharmacology.http://www.sciencedirect.com/science/article/pii/S1878535223009188Semen Cuscutae processed with salt solutionUHPLC-Q/TOF-MSKidney Deficiency MiscarriageNetwork pharmacology |
| spellingShingle | Xue Zhang Yu Huang Zhitong Yang Baiyang Xu Zilu Liu Ximeng Ding Qiumei Zhou Gang Cao Weidong Li Chuanshan Jin Shanshan Li Xiaoli Wang Jijun Chu Exploring the mechanism of Semen Cuscutae processed with salt solution in improving kidney deficiency miscarriage based on serum pharmacochemistry and network pharmacology Arabian Journal of Chemistry Semen Cuscutae processed with salt solution UHPLC-Q/TOF-MS Kidney Deficiency Miscarriage Network pharmacology |
| title | Exploring the mechanism of Semen Cuscutae processed with salt solution in improving kidney deficiency miscarriage based on serum pharmacochemistry and network pharmacology |
| title_full | Exploring the mechanism of Semen Cuscutae processed with salt solution in improving kidney deficiency miscarriage based on serum pharmacochemistry and network pharmacology |
| title_fullStr | Exploring the mechanism of Semen Cuscutae processed with salt solution in improving kidney deficiency miscarriage based on serum pharmacochemistry and network pharmacology |
| title_full_unstemmed | Exploring the mechanism of Semen Cuscutae processed with salt solution in improving kidney deficiency miscarriage based on serum pharmacochemistry and network pharmacology |
| title_short | Exploring the mechanism of Semen Cuscutae processed with salt solution in improving kidney deficiency miscarriage based on serum pharmacochemistry and network pharmacology |
| title_sort | exploring the mechanism of semen cuscutae processed with salt solution in improving kidney deficiency miscarriage based on serum pharmacochemistry and network pharmacology |
| topic | Semen Cuscutae processed with salt solution UHPLC-Q/TOF-MS Kidney Deficiency Miscarriage Network pharmacology |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223009188 |
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