Pneumococcal Serotype Identification by Capsular Sequence Typing (CST): A Modified Novel Approach for Serotyping Directly in Clinical Samples
As almost 60–70% of Invasive Pneumococcal Disease (IPD) is identified by nonculture methods in Greece, serotyping is of high importance for the better monitoring of pneumococcal serotypes due to the availability of conjugate vaccines. The aim of the study was the modification and direct application...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/12/2353 |
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| author | Nektarios Marmaras Athanasia Xirogianni Anastasia Papandreou Efthymia Petinaki Vana Papaevangelou Maria Tsolia Georgina Tzanakaki |
| author_facet | Nektarios Marmaras Athanasia Xirogianni Anastasia Papandreou Efthymia Petinaki Vana Papaevangelou Maria Tsolia Georgina Tzanakaki |
| author_sort | Nektarios Marmaras |
| collection | DOAJ |
| description | As almost 60–70% of Invasive Pneumococcal Disease (IPD) is identified by nonculture methods in Greece, serotyping is of high importance for the better monitoring of pneumococcal serotypes due to the availability of conjugate vaccines. The aim of the study was the modification and direct application of the Capsular Sequence Typing (CST) assay in clinical samples in order to serotype <i>Streptococcus pneumoniae</i> culture-negative, Polymerase Chain Reaction (PCR_-positive samples, followed by CST group specific single-tube PCR assays. A two-step PCR modified assay was applied on a total of 306 samples (such as CSF, blood, pleural and middle ear fluids, isolates) obtained from 283 patients with IPD. The overall performance permits a rapid, accurate and cost-effective method for nonculture pneumococcal serotyping. As the management of IPD is closely related to the continuous monitoring of pneumococcal serotypes, the proposed approach proved to be a valuable tool for the typing and epidemiological monitoring of <i>S. pneumoniae</i>, for the evaluation of the overall impact of vaccination programs in the era of pneumococcal conjugate vaccines, in order to initiate the appropriate vaccination strategy. |
| first_indexed | 2024-03-10T04:18:34Z |
| format | Article |
| id | doaj.art-03fb878c4c1e4f268083e9ed57f61cb0 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T04:18:34Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-03fb878c4c1e4f268083e9ed57f61cb02023-11-23T07:54:52ZengMDPI AGDiagnostics2075-44182021-12-011112235310.3390/diagnostics11122353Pneumococcal Serotype Identification by Capsular Sequence Typing (CST): A Modified Novel Approach for Serotyping Directly in Clinical SamplesNektarios Marmaras0Athanasia Xirogianni1Anastasia Papandreou2Efthymia Petinaki3Vana Papaevangelou4Maria Tsolia5Georgina Tzanakaki6National Meningitis Reference Laboratory, Department of Public Health Policy, School of Public Health, University of West Attica, 115 21 Athens, GreeceNational Meningitis Reference Laboratory, Department of Public Health Policy, School of Public Health, University of West Attica, 115 21 Athens, GreeceNational Meningitis Reference Laboratory, Department of Public Health Policy, School of Public Health, University of West Attica, 115 21 Athens, GreeceDepartment of Microbiology, Medical School, University of Thessaly, 411 10 Larissa, GreeceThird Department of Pediatrics, National and Kapodistrian University of Athens, University General Hospital “ATTIKON”, 124 62 Athens, GreeceSecond Department of Pediatrics, National and Kapodistrian University of Athens, “A&P Kyriakou” Children’s Hospital, 115 27 Athens, GreeceNational Meningitis Reference Laboratory, Department of Public Health Policy, School of Public Health, University of West Attica, 115 21 Athens, GreeceAs almost 60–70% of Invasive Pneumococcal Disease (IPD) is identified by nonculture methods in Greece, serotyping is of high importance for the better monitoring of pneumococcal serotypes due to the availability of conjugate vaccines. The aim of the study was the modification and direct application of the Capsular Sequence Typing (CST) assay in clinical samples in order to serotype <i>Streptococcus pneumoniae</i> culture-negative, Polymerase Chain Reaction (PCR_-positive samples, followed by CST group specific single-tube PCR assays. A two-step PCR modified assay was applied on a total of 306 samples (such as CSF, blood, pleural and middle ear fluids, isolates) obtained from 283 patients with IPD. The overall performance permits a rapid, accurate and cost-effective method for nonculture pneumococcal serotyping. As the management of IPD is closely related to the continuous monitoring of pneumococcal serotypes, the proposed approach proved to be a valuable tool for the typing and epidemiological monitoring of <i>S. pneumoniae</i>, for the evaluation of the overall impact of vaccination programs in the era of pneumococcal conjugate vaccines, in order to initiate the appropriate vaccination strategy.https://www.mdpi.com/2075-4418/11/12/2353<i>S. pneumoniae</i>non-culture serotypingcapsular sequence typing (CST) |
| spellingShingle | Nektarios Marmaras Athanasia Xirogianni Anastasia Papandreou Efthymia Petinaki Vana Papaevangelou Maria Tsolia Georgina Tzanakaki Pneumococcal Serotype Identification by Capsular Sequence Typing (CST): A Modified Novel Approach for Serotyping Directly in Clinical Samples Diagnostics <i>S. pneumoniae</i> non-culture serotyping capsular sequence typing (CST) |
| title | Pneumococcal Serotype Identification by Capsular Sequence Typing (CST): A Modified Novel Approach for Serotyping Directly in Clinical Samples |
| title_full | Pneumococcal Serotype Identification by Capsular Sequence Typing (CST): A Modified Novel Approach for Serotyping Directly in Clinical Samples |
| title_fullStr | Pneumococcal Serotype Identification by Capsular Sequence Typing (CST): A Modified Novel Approach for Serotyping Directly in Clinical Samples |
| title_full_unstemmed | Pneumococcal Serotype Identification by Capsular Sequence Typing (CST): A Modified Novel Approach for Serotyping Directly in Clinical Samples |
| title_short | Pneumococcal Serotype Identification by Capsular Sequence Typing (CST): A Modified Novel Approach for Serotyping Directly in Clinical Samples |
| title_sort | pneumococcal serotype identification by capsular sequence typing cst a modified novel approach for serotyping directly in clinical samples |
| topic | <i>S. pneumoniae</i> non-culture serotyping capsular sequence typing (CST) |
| url | https://www.mdpi.com/2075-4418/11/12/2353 |
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