The Enterococcus faecalis exoproteome: identification and temporal regulation by Fsr.
Analysis of the culture supernatant exoproteins produced by two PFGE clusters of high-level gentamicin and ciprofloxacin-resistant clinical isolates of Enterococcus faecalis from the UK and Ireland revealed two distinct protein profiles. This grouping distinguished OG1RF and GelE metalloprotease-exp...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3299793?pdf=render |
| _version_ | 1818837361101897728 |
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| author | Jayendra Shankar Rachel G Walker Deborah Ward Malcolm J Horsburgh |
| author_facet | Jayendra Shankar Rachel G Walker Deborah Ward Malcolm J Horsburgh |
| author_sort | Jayendra Shankar |
| collection | DOAJ |
| description | Analysis of the culture supernatant exoproteins produced by two PFGE clusters of high-level gentamicin and ciprofloxacin-resistant clinical isolates of Enterococcus faecalis from the UK and Ireland revealed two distinct protein profiles. This grouping distinguished OG1RF and GelE metalloprotease-expressing isolates from JH2-2 and other GelE-negative isolates. The integrity of the fsrABDC operon was found to determine the exoproteome composition, since an fsrB mutant of strain OG1RF appeared very similar to that of strain JH2-2, and complementation of the latter with the fsrABDC operon produced an OG1RF-like exoproteome. The proteins present in the supernatant fraction of OG1RF were separated using 2D gels and identified by mass spectrometry and comprised many mass and pI variants of the GelE and SprE proteases. In addition cell wall synthesis and cell division proteins were identified. An OG1RF fsrB mutant had a distinct exoprotein fraction with an absence of the Fsr-regulated proteases and was characterised by general stress and glycolytic proteins. The exoproteome of the OG1RF fsrB mutant resembles that of a divIVA mutant of E. faecalis, suggestive of a stress phenotype. |
| first_indexed | 2024-12-19T03:21:16Z |
| format | Article |
| id | doaj.art-03fc180dea8e4baab852a2694d101c14 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T03:21:16Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-03fc180dea8e4baab852a2694d101c142022-12-21T20:37:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3345010.1371/journal.pone.0033450The Enterococcus faecalis exoproteome: identification and temporal regulation by Fsr.Jayendra ShankarRachel G WalkerDeborah WardMalcolm J HorsburghAnalysis of the culture supernatant exoproteins produced by two PFGE clusters of high-level gentamicin and ciprofloxacin-resistant clinical isolates of Enterococcus faecalis from the UK and Ireland revealed two distinct protein profiles. This grouping distinguished OG1RF and GelE metalloprotease-expressing isolates from JH2-2 and other GelE-negative isolates. The integrity of the fsrABDC operon was found to determine the exoproteome composition, since an fsrB mutant of strain OG1RF appeared very similar to that of strain JH2-2, and complementation of the latter with the fsrABDC operon produced an OG1RF-like exoproteome. The proteins present in the supernatant fraction of OG1RF were separated using 2D gels and identified by mass spectrometry and comprised many mass and pI variants of the GelE and SprE proteases. In addition cell wall synthesis and cell division proteins were identified. An OG1RF fsrB mutant had a distinct exoprotein fraction with an absence of the Fsr-regulated proteases and was characterised by general stress and glycolytic proteins. The exoproteome of the OG1RF fsrB mutant resembles that of a divIVA mutant of E. faecalis, suggestive of a stress phenotype.http://europepmc.org/articles/PMC3299793?pdf=render |
| spellingShingle | Jayendra Shankar Rachel G Walker Deborah Ward Malcolm J Horsburgh The Enterococcus faecalis exoproteome: identification and temporal regulation by Fsr. PLoS ONE |
| title | The Enterococcus faecalis exoproteome: identification and temporal regulation by Fsr. |
| title_full | The Enterococcus faecalis exoproteome: identification and temporal regulation by Fsr. |
| title_fullStr | The Enterococcus faecalis exoproteome: identification and temporal regulation by Fsr. |
| title_full_unstemmed | The Enterococcus faecalis exoproteome: identification and temporal regulation by Fsr. |
| title_short | The Enterococcus faecalis exoproteome: identification and temporal regulation by Fsr. |
| title_sort | enterococcus faecalis exoproteome identification and temporal regulation by fsr |
| url | http://europepmc.org/articles/PMC3299793?pdf=render |
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