Process diagnosis for synthesizing reaction of N-acetyl-L-cysteine by thin layer chromatography(薄层色谱法对N-乙酰-L-半胱氨酸工艺流程诊断)
建立了用薄层层析和薄层扫描法对N-乙酰-L-半胱氨酸合成进行流程诊断的方法.采用硅胶G板,以正丁醇-醋酸-水(4.5∶1.5∶1)为展开剂,单波长直线扫描,扫描波长λ=700 nm.对合成反应的初始阶段、反应中、反应后取样样品进行定性和定量检测.原料在反应滴加后的初始阶段转化率达到97.08%,说明水相合成法较为合理,有效地抑制了L-半胱氨酸的氧化.设计的流程诊断法对N-乙酰-L-半胱氨酸和半胱氨酸盐酸盐的线性范围分别为15.1～36.5mg/mL、1.5～3.1mg/mL.平均回收率分别为99.93%～100.05%、99.94%～100.10%.RSD分别为0.32%～0.34%、0.31...
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2004-01-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/zjup/1008-9497.2004.31.1.62-65 |
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| author | CUIYan-li(崔艳丽) WANChang-chun(万长春) MAOJian-wei(毛建卫) |
| author_facet | CUIYan-li(崔艳丽) WANChang-chun(万长春) MAOJian-wei(毛建卫) |
| author_sort | CUIYan-li(崔艳丽) |
| collection | DOAJ |
| description | 建立了用薄层层析和薄层扫描法对N-乙酰-L-半胱氨酸合成进行流程诊断的方法.采用硅胶G板,以正丁醇-醋酸-水(4.5∶1.5∶1)为展开剂,单波长直线扫描,扫描波长λ=700 nm.对合成反应的初始阶段、反应中、反应后取样样品进行定性和定量检测.原料在反应滴加后的初始阶段转化率达到97.08%,说明水相合成法较为合理,有效地抑制了L-半胱氨酸的氧化.设计的流程诊断法对N-乙酰-L-半胱氨酸和半胱氨酸盐酸盐的线性范围分别为15.1～36.5mg/mL、1.5～3.1mg/mL.平均回收率分别为99.93%～100.05%、99.94%～100.10%.RSD分别为0.32%～0.34%、0.31%～0.34%.该方法可靠、简便易行,有利于反应流程诊断控制. |
| first_indexed | 2024-04-24T17:02:00Z |
| format | Article |
| id | doaj.art-03fc201a11914948a0ba120f2648795a |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T17:02:00Z |
| publishDate | 2004-01-01 |
| publisher | Zhejiang University Press |
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| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-03fc201a11914948a0ba120f2648795a2024-03-29T01:58:21ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972004-01-013116265zjup/1008-9497.2004.31.1.62-65Process diagnosis for synthesizing reaction of N-acetyl-L-cysteine by thin layer chromatography(薄层色谱法对N-乙酰-L-半胱氨酸工艺流程诊断)CUIYan-li(崔艳丽)0WANChang-chun(万长春)1MAOJian-wei(毛建卫)2 1.Department of Chemistry, Zhejiang University, Hangzhou 310027, China( 1.浙江大学化学系, 浙江 杭州 310027) 1.Department of Chemistry, Zhejiang University, Hangzhou 310027, China( 1.浙江大学化学系, 浙江 杭州 310027) 2.Department of Biology and Chemistry Engineering, Zhejiang University of Science and Technology, Hangzhou 310012, China( 2.浙江科技学院生物与化学工程学系, 浙江 杭州 310012)建立了用薄层层析和薄层扫描法对N-乙酰-L-半胱氨酸合成进行流程诊断的方法.采用硅胶G板,以正丁醇-醋酸-水(4.5∶1.5∶1)为展开剂,单波长直线扫描,扫描波长λ=700 nm.对合成反应的初始阶段、反应中、反应后取样样品进行定性和定量检测.原料在反应滴加后的初始阶段转化率达到97.08%,说明水相合成法较为合理,有效地抑制了L-半胱氨酸的氧化.设计的流程诊断法对N-乙酰-L-半胱氨酸和半胱氨酸盐酸盐的线性范围分别为15.1～36.5mg/mL、1.5～3.1mg/mL.平均回收率分别为99.93%～100.05%、99.94%～100.10%.RSD分别为0.32%～0.34%、0.31%～0.34%.该方法可靠、简便易行,有利于反应流程诊断控制.https://doi.org/zjup/1008-9497.2004.31.1.62-65薄层层析薄层扫描流程诊断n-乙酰-l-半胱氨酸 |
| spellingShingle | CUIYan-li(崔艳丽) WANChang-chun(万长春) MAOJian-wei(毛建卫) Process diagnosis for synthesizing reaction of N-acetyl-L-cysteine by thin layer chromatography(薄层色谱法对N-乙酰-L-半胱氨酸工艺流程诊断) Zhejiang Daxue xuebao. Lixue ban 薄层层析 薄层扫描 流程诊断 n-乙酰-l-半胱氨酸 |
| title | Process diagnosis for synthesizing reaction of N-acetyl-L-cysteine by thin layer chromatography(薄层色谱法对N-乙酰-L-半胱氨酸工艺流程诊断) |
| title_full | Process diagnosis for synthesizing reaction of N-acetyl-L-cysteine by thin layer chromatography(薄层色谱法对N-乙酰-L-半胱氨酸工艺流程诊断) |
| title_fullStr | Process diagnosis for synthesizing reaction of N-acetyl-L-cysteine by thin layer chromatography(薄层色谱法对N-乙酰-L-半胱氨酸工艺流程诊断) |
| title_full_unstemmed | Process diagnosis for synthesizing reaction of N-acetyl-L-cysteine by thin layer chromatography(薄层色谱法对N-乙酰-L-半胱氨酸工艺流程诊断) |
| title_short | Process diagnosis for synthesizing reaction of N-acetyl-L-cysteine by thin layer chromatography(薄层色谱法对N-乙酰-L-半胱氨酸工艺流程诊断) |
| title_sort | process diagnosis for synthesizing reaction of n acetyl l cysteine by thin layer chromatography 薄层色谱法对n 乙酰 l 半胱氨酸工艺流程诊断 |
| topic | 薄层层析 薄层扫描 流程诊断 n-乙酰-l-半胱氨酸 |
| url | https://doi.org/zjup/1008-9497.2004.31.1.62-65 |
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