Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infection

Interspecific hybrids of Prunus persica, Barier, Fire, Cadaman, GF-677, and Prunus sp. hybrids and selections, MRS, NBS 540-73, and Pumiselect were evaluated for resistance to Plum pox virus. Hybrids were grafted onto trees of a peach cultivar artificially infected with PPV and evaluated for six yea...

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Main Authors: Jaroslav Polák, Ivan Oukropec
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2010-12-01
Series:Plant Protection Science
Subjects:
Online Access:https://pps.agriculturejournals.cz/artkey/pps-201004-0002_identification-of-interspecific-peach-and-prunus-sp-hybrids-resistant-to-plum-pox-virus-infection.php
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author Jaroslav Polák
Ivan Oukropec
author_facet Jaroslav Polák
Ivan Oukropec
author_sort Jaroslav Polák
collection DOAJ
description Interspecific hybrids of Prunus persica, Barier, Fire, Cadaman, GF-677, and Prunus sp. hybrids and selections, MRS, NBS 540-73, and Pumiselect were evaluated for resistance to Plum pox virus. Hybrids were grafted onto trees of a peach cultivar artificially infected with PPV and evaluated for six years for resistance to the virus. The relative concentration of PPV protein was determined by semiquantitative ELISA in June every year. The presence of PPV in peach hybrids was confirmed by IC-RT-PCR in 2007-2008. The presence and intensity of PPV symptoms were evaluated monthly from May to September. The hybrid GF-677 (P. amygdalus × P. persica) was confirmed as highly resistant to PPV. Hybrids Cadaman (P. davidiana × P. persica) and Fire (P. amygdalus × P. persica) were characterized as resistant to PPV. Hybrids GF-677, Cadaman and Fire were selected as candidate sources of resistance to be crossed with peach cultivars susceptible to PPV.
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spelling doaj.art-03fdb51d9aac4a329640cd88b0f538ed2023-02-23T03:43:58ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412010-12-0146413914410.17221/11/2010-PPSpps-201004-0002Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infectionJaroslav Polák0Ivan Oukropec1Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech RepublicMendel University in Brno, Faculty of Horticulture, Lednice na Moravě, Czech RepublicInterspecific hybrids of Prunus persica, Barier, Fire, Cadaman, GF-677, and Prunus sp. hybrids and selections, MRS, NBS 540-73, and Pumiselect were evaluated for resistance to Plum pox virus. Hybrids were grafted onto trees of a peach cultivar artificially infected with PPV and evaluated for six years for resistance to the virus. The relative concentration of PPV protein was determined by semiquantitative ELISA in June every year. The presence of PPV in peach hybrids was confirmed by IC-RT-PCR in 2007-2008. The presence and intensity of PPV symptoms were evaluated monthly from May to September. The hybrid GF-677 (P. amygdalus × P. persica) was confirmed as highly resistant to PPV. Hybrids Cadaman (P. davidiana × P. persica) and Fire (P. amygdalus × P. persica) were characterized as resistant to PPV. Hybrids GF-677, Cadaman and Fire were selected as candidate sources of resistance to be crossed with peach cultivars susceptible to PPV.https://pps.agriculturejournals.cz/artkey/pps-201004-0002_identification-of-interspecific-peach-and-prunus-sp-hybrids-resistant-to-plum-pox-virus-infection.phpsharka diseasepeachprunus sp.sources of resistancedeterminationintensity of symptomssemiquantitative elisaic-rt-pcr detection
spellingShingle Jaroslav Polák
Ivan Oukropec
Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infection
Plant Protection Science
sharka disease
peach
prunus sp.
sources of resistance
determination
intensity of symptoms
semiquantitative elisa
ic-rt-pcr detection
title Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infection
title_full Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infection
title_fullStr Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infection
title_full_unstemmed Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infection
title_short Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infection
title_sort identification of interspecific peach and prunus sp hybrids resistant to plum pox virus infection
topic sharka disease
peach
prunus sp.
sources of resistance
determination
intensity of symptoms
semiquantitative elisa
ic-rt-pcr detection
url https://pps.agriculturejournals.cz/artkey/pps-201004-0002_identification-of-interspecific-peach-and-prunus-sp-hybrids-resistant-to-plum-pox-virus-infection.php
work_keys_str_mv AT jaroslavpolak identificationofinterspecificpeachandprunussphybridsresistanttoplumpoxvirusinfection
AT ivanoukropec identificationofinterspecificpeachandprunussphybridsresistanttoplumpoxvirusinfection