NMDA modulates oligodendrocyte differentiation of subventricular zone cells through PKC activation
Multipotent cells from the juvenile subventricular zone (SVZ) possess the ability to differentiate into new neural cells. Depending on local signals, SVZ can generate new neurons, astrocytes or oligodendrocytes. We previously demonstrated that activation of NMDA receptors in SVZ progenitors increase...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-12-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00261/full |
| _version_ | 1818415464649326592 |
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| author | Fabio eCavaliere Monica eBenito-Muñoz Mitradas ePanicker Carlos eMatute |
| author_facet | Fabio eCavaliere Monica eBenito-Muñoz Mitradas ePanicker Carlos eMatute |
| author_sort | Fabio eCavaliere |
| collection | DOAJ |
| description | Multipotent cells from the juvenile subventricular zone (SVZ) possess the ability to differentiate into new neural cells. Depending on local signals, SVZ can generate new neurons, astrocytes or oligodendrocytes. We previously demonstrated that activation of NMDA receptors in SVZ progenitors increases the rate of oligodendrocyte differentiation. Here we investigated the mechanisms involved in NMDA receptor-dependent differentiation. Using functional studies performed with the reporter gene luciferase we found that activation of NMDA receptor stimulates PKC. In turn, stimulation of PKC precedes the activation of NADPH oxidase (NOX) as demonstrated by translocation of the p67phox subunit to the cellular membrane. We propose that NOX2 is involved in the transduction of the signal from NMDA receptors through PKC activation as the inhibitor gp91 reduced their pro-differentiation effect. In addition, our data and that from other groups suggest that signaling through the NMDA receptor/PKC/NOX2 cascade generates ROS that activate the PI3/mTOR pathway and finally leads to the generation of new oligodendrocytes. |
| first_indexed | 2024-12-14T11:35:25Z |
| format | Article |
| id | doaj.art-03fe37e7bbe649f48e5b78084582f6be |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-14T11:35:25Z |
| publishDate | 2013-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-03fe37e7bbe649f48e5b78084582f6be2022-12-21T23:03:03ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022013-12-01710.3389/fncel.2013.0026163136NMDA modulates oligodendrocyte differentiation of subventricular zone cells through PKC activationFabio eCavaliere0Monica eBenito-Muñoz1Mitradas ePanicker2Carlos eMatute3Universidad del Pais Vasco (UPV/EHU)Universidad del Pais Vasco (UPV/EHU)National Centre for Biological Sciences, UAS-GKVK CampusUniversidad del Pais Vasco (UPV/EHU)Multipotent cells from the juvenile subventricular zone (SVZ) possess the ability to differentiate into new neural cells. Depending on local signals, SVZ can generate new neurons, astrocytes or oligodendrocytes. We previously demonstrated that activation of NMDA receptors in SVZ progenitors increases the rate of oligodendrocyte differentiation. Here we investigated the mechanisms involved in NMDA receptor-dependent differentiation. Using functional studies performed with the reporter gene luciferase we found that activation of NMDA receptor stimulates PKC. In turn, stimulation of PKC precedes the activation of NADPH oxidase (NOX) as demonstrated by translocation of the p67phox subunit to the cellular membrane. We propose that NOX2 is involved in the transduction of the signal from NMDA receptors through PKC activation as the inhibitor gp91 reduced their pro-differentiation effect. In addition, our data and that from other groups suggest that signaling through the NMDA receptor/PKC/NOX2 cascade generates ROS that activate the PI3/mTOR pathway and finally leads to the generation of new oligodendrocytes.http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00261/fullNADPH OxidaseReactive Oxygen SpeciesNMDAPKCMultipotent cell differentiation |
| spellingShingle | Fabio eCavaliere Monica eBenito-Muñoz Mitradas ePanicker Carlos eMatute NMDA modulates oligodendrocyte differentiation of subventricular zone cells through PKC activation Frontiers in Cellular Neuroscience NADPH Oxidase Reactive Oxygen Species NMDA PKC Multipotent cell differentiation |
| title | NMDA modulates oligodendrocyte differentiation of subventricular zone cells through PKC activation |
| title_full | NMDA modulates oligodendrocyte differentiation of subventricular zone cells through PKC activation |
| title_fullStr | NMDA modulates oligodendrocyte differentiation of subventricular zone cells through PKC activation |
| title_full_unstemmed | NMDA modulates oligodendrocyte differentiation of subventricular zone cells through PKC activation |
| title_short | NMDA modulates oligodendrocyte differentiation of subventricular zone cells through PKC activation |
| title_sort | nmda modulates oligodendrocyte differentiation of subventricular zone cells through pkc activation |
| topic | NADPH Oxidase Reactive Oxygen Species NMDA PKC Multipotent cell differentiation |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00261/full |
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