Micropropagation of Asparagus officinalis L. through callus development
In order to develop an in vitro propagation process for production and conservation, in this study, we investigated the influence of disinfectants and plants growth regulators (PGR) on the infection rate of explants, the ability of callus formation, and the regeneration of shoot and root from Aspara...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2022-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2022/06/bioconf_itia2022_04001.pdf |
| _version_ | 1811342749444603904 |
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| author | Nguyen Minh Ly Nguyen Thi Nhat Tuyen Le Huynh Diem Suong Mironov Aleksey Vorobyev Mikhail Orlova Elena |
| author_facet | Nguyen Minh Ly Nguyen Thi Nhat Tuyen Le Huynh Diem Suong Mironov Aleksey Vorobyev Mikhail Orlova Elena |
| author_sort | Nguyen Minh Ly |
| collection | DOAJ |
| description | In order to develop an in vitro propagation process for production and conservation, in this study, we investigated the influence of disinfectants and plants growth regulators (PGR) on the infection rate of explants, the ability of callus formation, and the regeneration of shoot and root from Asparagus officinalis L. trunk segments. We obtained the best results when disinfecting trunk segments explants combined with alcohol 70° in 5 minutes and NaClO in 5 minutes with the lowest contamination rate of 3.7%. Callus was formed well in MS medium supplemented with 3% sucrose; 0.7% agar, pH= 5.8; 1.0 mg/l BAP and combined with 0.5 mg/l NAA, 1.5 mg/l BAP and 0.75 mg/l NAA. The highest rate of shoot formation was 87.2% when the callus continued to grow in the environment supplemented with 2.0 mg/l BAP and 1.0 mg/l NAA. Roots were formed after 4 weeks of shoot cultivation in MS medium supplemented with 0.5 mg/l NAA with the highest rooting rate of 74.59%. |
| first_indexed | 2024-04-13T19:16:22Z |
| format | Article |
| id | doaj.art-03fe7677198f4272b2de2c66f28951ed |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-04-13T19:16:22Z |
| publishDate | 2022-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-03fe7677198f4272b2de2c66f28951ed2022-12-22T02:33:39ZengEDP SciencesBIO Web of Conferences2117-44582022-01-01470400110.1051/bioconf/20224704001bioconf_itia2022_04001Micropropagation of Asparagus officinalis L. through callus developmentNguyen Minh Ly0Nguyen Thi Nhat Tuyen1Le Huynh Diem Suong2Mironov Aleksey3Vorobyev Mikhail4Orlova Elena5The University of Danang-University of Science and Education, ul. Ton Duc ThangThe University of Danang-University of Science and Education, ul. Ton Duc ThangThe University of Danang-University of Science and Education, ul. Ton Duc ThangRussian State University - Moscow Agricultural Academy named after K. A. TimiryazevRussian State University - Moscow Agricultural Academy named after K. A. TimiryazevRussian State University - Moscow Agricultural Academy named after K. A. TimiryazevIn order to develop an in vitro propagation process for production and conservation, in this study, we investigated the influence of disinfectants and plants growth regulators (PGR) on the infection rate of explants, the ability of callus formation, and the regeneration of shoot and root from Asparagus officinalis L. trunk segments. We obtained the best results when disinfecting trunk segments explants combined with alcohol 70° in 5 minutes and NaClO in 5 minutes with the lowest contamination rate of 3.7%. Callus was formed well in MS medium supplemented with 3% sucrose; 0.7% agar, pH= 5.8; 1.0 mg/l BAP and combined with 0.5 mg/l NAA, 1.5 mg/l BAP and 0.75 mg/l NAA. The highest rate of shoot formation was 87.2% when the callus continued to grow in the environment supplemented with 2.0 mg/l BAP and 1.0 mg/l NAA. Roots were formed after 4 weeks of shoot cultivation in MS medium supplemented with 0.5 mg/l NAA with the highest rooting rate of 74.59%.https://www.bio-conferences.org/articles/bioconf/pdf/2022/06/bioconf_itia2022_04001.pdf |
| spellingShingle | Nguyen Minh Ly Nguyen Thi Nhat Tuyen Le Huynh Diem Suong Mironov Aleksey Vorobyev Mikhail Orlova Elena Micropropagation of Asparagus officinalis L. through callus development BIO Web of Conferences |
| title | Micropropagation of Asparagus officinalis L. through callus development |
| title_full | Micropropagation of Asparagus officinalis L. through callus development |
| title_fullStr | Micropropagation of Asparagus officinalis L. through callus development |
| title_full_unstemmed | Micropropagation of Asparagus officinalis L. through callus development |
| title_short | Micropropagation of Asparagus officinalis L. through callus development |
| title_sort | micropropagation of asparagus officinalis l through callus development |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2022/06/bioconf_itia2022_04001.pdf |
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