Estradiol as an antioxidant: incompatible with its physiological concentrations and function
Estradiol has been documented to inhibit the oxidation of low density lipoprotein (LDL). We show that physiological concentrations of estradiol do not inhibit the oxidation of LDL by copper. LDL samples isolated from a) premenopausal and postmenopausal women and from b) women at different time perio...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1998-11-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520324652 |
| _version_ | 1818961354850041856 |
|---|---|
| author | Nalini Santanam Robin Shern-Brewer Ruth McClatchey Penny Z. Castellano Ana A. Murphy Steve Voelkel Sampath Parthasarathy |
| author_facet | Nalini Santanam Robin Shern-Brewer Ruth McClatchey Penny Z. Castellano Ana A. Murphy Steve Voelkel Sampath Parthasarathy |
| author_sort | Nalini Santanam |
| collection | DOAJ |
| description | Estradiol has been documented to inhibit the oxidation of low density lipoprotein (LDL). We show that physiological concentrations of estradiol do not inhibit the oxidation of LDL by copper. LDL samples isolated from a) premenopausal and postmenopausal women and from b) women at different time periods during their menstrual cycle, who differ vastly in plasma estradiol levels, were also oxidized at the same rates by copper. In contrast, LDL samples isolated from c) women who were hyperstimulated during in vitro fertilization (IVF), with estradiol concentrations above 2000 pg/ml, were resistant to oxidation by copper. However, these LDL samples were also oxidized at a higher rate by peroxidases. More importantly, subjects with high estradiol levels also showed an increase in myeloperoxidase (MPO) protein in the plasma. Based on these results, we conclude that at physiologic concentrations, it is unlikely that estradiol could act as an antioxidant. In fact, the ability of estradiol to induce MPO and become a prooxidant might instead suggest that MPO-mediated oxidative clearance of LDL from plasma by liver might favorably influence the outcome of atherosclerosis. —Santanam, N., R. Shern-Brewer, R. McClatchey, P. Z. Castellano, A. A. Murphy, S. Voelkel, and S. Parthasarathy. Estradiol as an antioxidant: incompatible with its physiological concentrations and function. J. Lipid Res. 1998. 39: 2111–2118. |
| first_indexed | 2024-12-20T12:12:06Z |
| format | Article |
| id | doaj.art-03fef592f42943bd9b5d341b5d986f49 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T12:12:06Z |
| publishDate | 1998-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-03fef592f42943bd9b5d341b5d986f492022-12-21T19:41:13ZengElsevierJournal of Lipid Research0022-22751998-11-01391121112118Estradiol as an antioxidant: incompatible with its physiological concentrations and functionNalini Santanam0Robin Shern-Brewer1Ruth McClatchey2Penny Z. Castellano3Ana A. Murphy4Steve Voelkel5Sampath Parthasarathy6Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA 30322Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA 30322Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA 30322Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA 30322Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA 30322Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA 30322To whom correspondence should be addressed.; Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA 30322Estradiol has been documented to inhibit the oxidation of low density lipoprotein (LDL). We show that physiological concentrations of estradiol do not inhibit the oxidation of LDL by copper. LDL samples isolated from a) premenopausal and postmenopausal women and from b) women at different time periods during their menstrual cycle, who differ vastly in plasma estradiol levels, were also oxidized at the same rates by copper. In contrast, LDL samples isolated from c) women who were hyperstimulated during in vitro fertilization (IVF), with estradiol concentrations above 2000 pg/ml, were resistant to oxidation by copper. However, these LDL samples were also oxidized at a higher rate by peroxidases. More importantly, subjects with high estradiol levels also showed an increase in myeloperoxidase (MPO) protein in the plasma. Based on these results, we conclude that at physiologic concentrations, it is unlikely that estradiol could act as an antioxidant. In fact, the ability of estradiol to induce MPO and become a prooxidant might instead suggest that MPO-mediated oxidative clearance of LDL from plasma by liver might favorably influence the outcome of atherosclerosis. —Santanam, N., R. Shern-Brewer, R. McClatchey, P. Z. Castellano, A. A. Murphy, S. Voelkel, and S. Parthasarathy. Estradiol as an antioxidant: incompatible with its physiological concentrations and function. J. Lipid Res. 1998. 39: 2111–2118.http://www.sciencedirect.com/science/article/pii/S0022227520324652atherosclerosismenopausecoronary artery diseaselipid peroxidationmyeloperoxidaseoxidized low-density lipoprotein |
| spellingShingle | Nalini Santanam Robin Shern-Brewer Ruth McClatchey Penny Z. Castellano Ana A. Murphy Steve Voelkel Sampath Parthasarathy Estradiol as an antioxidant: incompatible with its physiological concentrations and function Journal of Lipid Research atherosclerosis menopause coronary artery disease lipid peroxidation myeloperoxidase oxidized low-density lipoprotein |
| title | Estradiol as an antioxidant: incompatible with its physiological concentrations and function |
| title_full | Estradiol as an antioxidant: incompatible with its physiological concentrations and function |
| title_fullStr | Estradiol as an antioxidant: incompatible with its physiological concentrations and function |
| title_full_unstemmed | Estradiol as an antioxidant: incompatible with its physiological concentrations and function |
| title_short | Estradiol as an antioxidant: incompatible with its physiological concentrations and function |
| title_sort | estradiol as an antioxidant incompatible with its physiological concentrations and function |
| topic | atherosclerosis menopause coronary artery disease lipid peroxidation myeloperoxidase oxidized low-density lipoprotein |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520324652 |
| work_keys_str_mv | AT nalinisantanam estradiolasanantioxidantincompatiblewithitsphysiologicalconcentrationsandfunction AT robinshernbrewer estradiolasanantioxidantincompatiblewithitsphysiologicalconcentrationsandfunction AT ruthmcclatchey estradiolasanantioxidantincompatiblewithitsphysiologicalconcentrationsandfunction AT pennyzcastellano estradiolasanantioxidantincompatiblewithitsphysiologicalconcentrationsandfunction AT anaamurphy estradiolasanantioxidantincompatiblewithitsphysiologicalconcentrationsandfunction AT stevevoelkel estradiolasanantioxidantincompatiblewithitsphysiologicalconcentrationsandfunction AT sampathparthasarathy estradiolasanantioxidantincompatiblewithitsphysiologicalconcentrationsandfunction |