In vivo imaging of Hedgehog pathway activation with a nuclear fluorescent reporter.
The Hedgehog (Hh) pathway is essential for embryonic development and tissue regeneration, and its dysregulation can lead to birth defects and tumorigenesis. Understanding how this signaling mechanism contributes to these processes would benefit from an ability to visualize Hedgehog pathway activity...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4113417?pdf=render |
| _version_ | 1818940538739490816 |
|---|---|
| author | John K Mich Alexander Y Payumo Paul G Rack James K Chen |
| author_facet | John K Mich Alexander Y Payumo Paul G Rack James K Chen |
| author_sort | John K Mich |
| collection | DOAJ |
| description | The Hedgehog (Hh) pathway is essential for embryonic development and tissue regeneration, and its dysregulation can lead to birth defects and tumorigenesis. Understanding how this signaling mechanism contributes to these processes would benefit from an ability to visualize Hedgehog pathway activity in live organisms, in real time, and with single-cell resolution. We report here the generation of transgenic zebrafish lines that express nuclear-localized mCherry fluorescent protein in a Gli transcription factor-dependent manner. As demonstrated by chemical and genetic perturbations, these lines faithfully report Hedgehog pathway state in individual cells and with high detection sensitivity. They will be valuable tools for studying dynamic Gli-dependent processes in vertebrates and for identifying new chemical and genetic regulators of the Hh pathway. |
| first_indexed | 2024-12-20T06:41:14Z |
| format | Article |
| id | doaj.art-04012543a2c54f2c9e5f018c4ecbef18 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T06:41:14Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-04012543a2c54f2c9e5f018c4ecbef182022-12-21T19:49:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0197e10366110.1371/journal.pone.0103661In vivo imaging of Hedgehog pathway activation with a nuclear fluorescent reporter.John K MichAlexander Y PayumoPaul G RackJames K ChenThe Hedgehog (Hh) pathway is essential for embryonic development and tissue regeneration, and its dysregulation can lead to birth defects and tumorigenesis. Understanding how this signaling mechanism contributes to these processes would benefit from an ability to visualize Hedgehog pathway activity in live organisms, in real time, and with single-cell resolution. We report here the generation of transgenic zebrafish lines that express nuclear-localized mCherry fluorescent protein in a Gli transcription factor-dependent manner. As demonstrated by chemical and genetic perturbations, these lines faithfully report Hedgehog pathway state in individual cells and with high detection sensitivity. They will be valuable tools for studying dynamic Gli-dependent processes in vertebrates and for identifying new chemical and genetic regulators of the Hh pathway.http://europepmc.org/articles/PMC4113417?pdf=render |
| spellingShingle | John K Mich Alexander Y Payumo Paul G Rack James K Chen In vivo imaging of Hedgehog pathway activation with a nuclear fluorescent reporter. PLoS ONE |
| title | In vivo imaging of Hedgehog pathway activation with a nuclear fluorescent reporter. |
| title_full | In vivo imaging of Hedgehog pathway activation with a nuclear fluorescent reporter. |
| title_fullStr | In vivo imaging of Hedgehog pathway activation with a nuclear fluorescent reporter. |
| title_full_unstemmed | In vivo imaging of Hedgehog pathway activation with a nuclear fluorescent reporter. |
| title_short | In vivo imaging of Hedgehog pathway activation with a nuclear fluorescent reporter. |
| title_sort | in vivo imaging of hedgehog pathway activation with a nuclear fluorescent reporter |
| url | http://europepmc.org/articles/PMC4113417?pdf=render |
| work_keys_str_mv | AT johnkmich invivoimagingofhedgehogpathwayactivationwithanuclearfluorescentreporter AT alexanderypayumo invivoimagingofhedgehogpathwayactivationwithanuclearfluorescentreporter AT paulgrack invivoimagingofhedgehogpathwayactivationwithanuclearfluorescentreporter AT jameskchen invivoimagingofhedgehogpathwayactivationwithanuclearfluorescentreporter |