Effects of dosing non-toxigenic Clostridia on the bacterial populations and immunological responses in the intestinal tract of lactating dairy cows
Understanding the effects of dosing non-toxigenic Clostridia to cows is rare and has received little attention so far. In the present study, a total of eight lactating dairy cows were divided in two groups: control (n = 4) or Clostridia challenged (oral supplementation of five diverse strains of Par...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-06-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1107964/full |
| _version_ | 1797798379385782272 |
|---|---|
| author | Hye Won Kim Na Kyung Kim Jesse Thompson Mackenzie de Jesus Josh Rehberger Thomas Rehberger Alexandra Helena Smith Roderick Ian Mackie Roderick Ian Mackie |
| author_facet | Hye Won Kim Na Kyung Kim Jesse Thompson Mackenzie de Jesus Josh Rehberger Thomas Rehberger Alexandra Helena Smith Roderick Ian Mackie Roderick Ian Mackie |
| author_sort | Hye Won Kim |
| collection | DOAJ |
| description | Understanding the effects of dosing non-toxigenic Clostridia to cows is rare and has received little attention so far. In the present study, a total of eight lactating dairy cows were divided in two groups: control (n = 4) or Clostridia challenged (oral supplementation of five diverse strains of Paraclostridium bifermentans, n = 4). Bacterial communities were analyzed by qPCR and next-generation sequencing (NGS) in the buccal mucosa as well as digesta and mucosal samples of the gastrointestinal (GI) tract from rumen to rectum (10 compartments), as well as fecal samples. Transcriptomic analysis of barrier and immune-related gene expression was performed on rumen, jejunum, and liver samples. We observed increased microbial populations with the Clostridial challenge in the buccal tissues and the proximal GI tract (forestomach), correlating with Clostridial loads in the feed. Otherwise, there were no significant differences in microbial populations (p > 0.05) throughout the distal part of the GI tract. The NGS approach, however, revealed that the Clostridial challenge changed the relative abundance of gut and fecal microbiota. In particular, in the challenge group, no Bifidobacterium was observed in the mucosa-associated microbiota and abundance of Pseudomonadota increased in the feces. These results indicated potential adverse effects of Clostridia to cow health. In general, immune responses to the Clostridial challenge were weak. However, transcriptional analysis revealed the down-regulation of junction adhesion molecule encoding gene (−1.44 of log2 fold-change), which might impact intestinal permeability. |
| first_indexed | 2024-03-13T04:03:47Z |
| format | Article |
| id | doaj.art-04027fc4388146c8a5e2ba81230ab832 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T04:03:47Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-04027fc4388146c8a5e2ba81230ab8322023-06-21T10:25:17ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-06-011410.3389/fmicb.2023.11079641107964Effects of dosing non-toxigenic Clostridia on the bacterial populations and immunological responses in the intestinal tract of lactating dairy cowsHye Won Kim0Na Kyung Kim1Jesse Thompson2Mackenzie de Jesus3Josh Rehberger4Thomas Rehberger5Alexandra Helena Smith6Roderick Ian Mackie7Roderick Ian Mackie8Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesDepartment of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesArm & Hammer Animal and Food Production, Waukesha, WI, United StatesArm & Hammer Animal and Food Production, Waukesha, WI, United StatesArm & Hammer Animal and Food Production, Waukesha, WI, United StatesArm & Hammer Animal and Food Production, Waukesha, WI, United StatesArm & Hammer Animal and Food Production, Waukesha, WI, United StatesDepartment of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesCarle R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesUnderstanding the effects of dosing non-toxigenic Clostridia to cows is rare and has received little attention so far. In the present study, a total of eight lactating dairy cows were divided in two groups: control (n = 4) or Clostridia challenged (oral supplementation of five diverse strains of Paraclostridium bifermentans, n = 4). Bacterial communities were analyzed by qPCR and next-generation sequencing (NGS) in the buccal mucosa as well as digesta and mucosal samples of the gastrointestinal (GI) tract from rumen to rectum (10 compartments), as well as fecal samples. Transcriptomic analysis of barrier and immune-related gene expression was performed on rumen, jejunum, and liver samples. We observed increased microbial populations with the Clostridial challenge in the buccal tissues and the proximal GI tract (forestomach), correlating with Clostridial loads in the feed. Otherwise, there were no significant differences in microbial populations (p > 0.05) throughout the distal part of the GI tract. The NGS approach, however, revealed that the Clostridial challenge changed the relative abundance of gut and fecal microbiota. In particular, in the challenge group, no Bifidobacterium was observed in the mucosa-associated microbiota and abundance of Pseudomonadota increased in the feces. These results indicated potential adverse effects of Clostridia to cow health. In general, immune responses to the Clostridial challenge were weak. However, transcriptional analysis revealed the down-regulation of junction adhesion molecule encoding gene (−1.44 of log2 fold-change), which might impact intestinal permeability.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1107964/fullClostridialactating cowsdosingintestinal tractimmune function |
| spellingShingle | Hye Won Kim Na Kyung Kim Jesse Thompson Mackenzie de Jesus Josh Rehberger Thomas Rehberger Alexandra Helena Smith Roderick Ian Mackie Roderick Ian Mackie Effects of dosing non-toxigenic Clostridia on the bacterial populations and immunological responses in the intestinal tract of lactating dairy cows Frontiers in Microbiology Clostridia lactating cows dosing intestinal tract immune function |
| title | Effects of dosing non-toxigenic Clostridia on the bacterial populations and immunological responses in the intestinal tract of lactating dairy cows |
| title_full | Effects of dosing non-toxigenic Clostridia on the bacterial populations and immunological responses in the intestinal tract of lactating dairy cows |
| title_fullStr | Effects of dosing non-toxigenic Clostridia on the bacterial populations and immunological responses in the intestinal tract of lactating dairy cows |
| title_full_unstemmed | Effects of dosing non-toxigenic Clostridia on the bacterial populations and immunological responses in the intestinal tract of lactating dairy cows |
| title_short | Effects of dosing non-toxigenic Clostridia on the bacterial populations and immunological responses in the intestinal tract of lactating dairy cows |
| title_sort | effects of dosing non toxigenic clostridia on the bacterial populations and immunological responses in the intestinal tract of lactating dairy cows |
| topic | Clostridia lactating cows dosing intestinal tract immune function |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1107964/full |
| work_keys_str_mv | AT hyewonkim effectsofdosingnontoxigenicclostridiaonthebacterialpopulationsandimmunologicalresponsesintheintestinaltractoflactatingdairycows AT nakyungkim effectsofdosingnontoxigenicclostridiaonthebacterialpopulationsandimmunologicalresponsesintheintestinaltractoflactatingdairycows AT jessethompson effectsofdosingnontoxigenicclostridiaonthebacterialpopulationsandimmunologicalresponsesintheintestinaltractoflactatingdairycows AT mackenziedejesus effectsofdosingnontoxigenicclostridiaonthebacterialpopulationsandimmunologicalresponsesintheintestinaltractoflactatingdairycows AT joshrehberger effectsofdosingnontoxigenicclostridiaonthebacterialpopulationsandimmunologicalresponsesintheintestinaltractoflactatingdairycows AT thomasrehberger effectsofdosingnontoxigenicclostridiaonthebacterialpopulationsandimmunologicalresponsesintheintestinaltractoflactatingdairycows AT alexandrahelenasmith effectsofdosingnontoxigenicclostridiaonthebacterialpopulationsandimmunologicalresponsesintheintestinaltractoflactatingdairycows AT roderickianmackie effectsofdosingnontoxigenicclostridiaonthebacterialpopulationsandimmunologicalresponsesintheintestinaltractoflactatingdairycows AT roderickianmackie effectsofdosingnontoxigenicclostridiaonthebacterialpopulationsandimmunologicalresponsesintheintestinaltractoflactatingdairycows |