Identification of SSR markers linked to Botrytis grey mould resistance in chickpea (Cicer arietinum)
Botrytis grey mould (BGM), caused by Botrytis cinerea, is emerging as an important disease of chickpea in the northern and eastern parts of the Indian Subcontinent, including Nepal, Bangladesh, Pakistan, and in Australia. This fungus has a very broad host range, and sources of complete resistance to...
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| Format: | Article |
| Language: | English |
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Firenze University Press
2019-09-01
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| Series: | Phytopathologia Mediterranea |
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| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/10616 |
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| author | Supriya Sachdeva Shruti Dawar Upasana Rani Basavanagouda Siddanagouda Patil Khela Ram Soren Sarvjeet Singh Satish Kumar Sanwal Sanjeev K. Chauhan Chellapilla Bharadwaj |
| author_facet | Supriya Sachdeva Shruti Dawar Upasana Rani Basavanagouda Siddanagouda Patil Khela Ram Soren Sarvjeet Singh Satish Kumar Sanwal Sanjeev K. Chauhan Chellapilla Bharadwaj |
| author_sort | Supriya Sachdeva |
| collection | DOAJ |
| description | Botrytis grey mould (BGM), caused by Botrytis cinerea, is emerging as an important disease of chickpea in the northern and eastern parts of the Indian Subcontinent, including Nepal, Bangladesh, Pakistan, and in Australia. This fungus has a very broad host range, and sources of complete resistance to the disease have not been found in Cicer arietinum L. germplasm. Resistance to this pathogen has been identified in some wild Cicer species. A set of 371 lines, including 164 landraces and 207 interspecific derivative lines (derived from crosses of cultivated chickpea with C. pinnatifidum, C. judaicum or C. reticulatum) have been screened against Botrytis grey mould under field conditions, and using the cut twig method at the Punjab Agricultural University (PAU), Ludhiana, in 2015-16 and 2016–17. Strong correlations between the two screening methods were indicated by paired-t tests. The Bulked Sample Analysis (BSA) approach was used to screen DNA of the five most resistant and five most susceptible host lines using 300 simple sequence repeat (SSR) markers. Eighty-eight markers were polymorphic. Chi-square statistic values showed strong correlations of TA144, GA102, TA194, TA140 and TR2 with the resistant bulks, signifying their usability as putative markers linked to BGM resistance, and for development of BGM tolerant genotypes in chickpea. Future studies should rapidly ascertain marker trait associations, and identify and develop diagnostic markers that provide an accurate method of molecular tagging BGM resistant genes in chickpea. |
| first_indexed | 2024-04-13T12:51:43Z |
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| institution | Directory Open Access Journal |
| issn | 0031-9465 1593-2095 |
| language | English |
| last_indexed | 2024-04-13T12:51:43Z |
| publishDate | 2019-09-01 |
| publisher | Firenze University Press |
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| series | Phytopathologia Mediterranea |
| spelling | doaj.art-0406f4f2af6e4dd89b34f04411dd5e8d2022-12-22T02:46:11ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952019-09-0158210.14601/Phytopathol_Mediter-10616Identification of SSR markers linked to Botrytis grey mould resistance in chickpea (Cicer arietinum)Supriya Sachdeva0Shruti Dawar1Upasana Rani2Basavanagouda Siddanagouda Patil3Khela Ram Soren4Sarvjeet Singh5Satish Kumar Sanwal6Sanjeev K. Chauhan7Chellapilla Bharadwaj8Division of Genetics, Indian Agricultural Research Institute (ICAR), Pusa, New Delhi 110012Principal Pulse Breeder, Division of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, IndiaPrincipal Pulse Breeder, Division of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, IndiaDivision of Genetics, Indian Agricultural Research Institute (ICAR), Pusa, New Delhi 110012, IndiaICAR-Indian Institute of Pulse Research, Kanpur, UP, IndiaPrincipal Pulse Breeder, Division of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, IndiaICAR-CSSRI, Karnal, Haryana, IndiaDivision of Genetics, Indian Agricultural Research Institute (ICAR), Pusa, New Delhi 110012, IndiaDivision of Genetics, Indian Agricultural Research Institute (ICAR), Pusa, New Delhi 110012, IndiaBotrytis grey mould (BGM), caused by Botrytis cinerea, is emerging as an important disease of chickpea in the northern and eastern parts of the Indian Subcontinent, including Nepal, Bangladesh, Pakistan, and in Australia. This fungus has a very broad host range, and sources of complete resistance to the disease have not been found in Cicer arietinum L. germplasm. Resistance to this pathogen has been identified in some wild Cicer species. A set of 371 lines, including 164 landraces and 207 interspecific derivative lines (derived from crosses of cultivated chickpea with C. pinnatifidum, C. judaicum or C. reticulatum) have been screened against Botrytis grey mould under field conditions, and using the cut twig method at the Punjab Agricultural University (PAU), Ludhiana, in 2015-16 and 2016–17. Strong correlations between the two screening methods were indicated by paired-t tests. The Bulked Sample Analysis (BSA) approach was used to screen DNA of the five most resistant and five most susceptible host lines using 300 simple sequence repeat (SSR) markers. Eighty-eight markers were polymorphic. Chi-square statistic values showed strong correlations of TA144, GA102, TA194, TA140 and TR2 with the resistant bulks, signifying their usability as putative markers linked to BGM resistance, and for development of BGM tolerant genotypes in chickpea. Future studies should rapidly ascertain marker trait associations, and identify and develop diagnostic markers that provide an accurate method of molecular tagging BGM resistant genes in chickpea.https://oajournals.fupress.net/index.php/pm/article/view/10616ChickpeaGrey mould resistanceSSR markersBulked sample analysis |
| spellingShingle | Supriya Sachdeva Shruti Dawar Upasana Rani Basavanagouda Siddanagouda Patil Khela Ram Soren Sarvjeet Singh Satish Kumar Sanwal Sanjeev K. Chauhan Chellapilla Bharadwaj Identification of SSR markers linked to Botrytis grey mould resistance in chickpea (Cicer arietinum) Phytopathologia Mediterranea Chickpea Grey mould resistance SSR markers Bulked sample analysis |
| title | Identification of SSR markers linked to Botrytis grey mould resistance in chickpea (Cicer arietinum) |
| title_full | Identification of SSR markers linked to Botrytis grey mould resistance in chickpea (Cicer arietinum) |
| title_fullStr | Identification of SSR markers linked to Botrytis grey mould resistance in chickpea (Cicer arietinum) |
| title_full_unstemmed | Identification of SSR markers linked to Botrytis grey mould resistance in chickpea (Cicer arietinum) |
| title_short | Identification of SSR markers linked to Botrytis grey mould resistance in chickpea (Cicer arietinum) |
| title_sort | identification of ssr markers linked to botrytis grey mould resistance in chickpea cicer arietinum |
| topic | Chickpea Grey mould resistance SSR markers Bulked sample analysis |
| url | https://oajournals.fupress.net/index.php/pm/article/view/10616 |
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