DNA methylation signatures of breast cancer in peripheral T-cells
Abstract Background Immune surveillance acts as a defense mechanism in cancer, and its disruption is involved in cancer progression. DNA methylation reflects the phenotypic identity of cells and recent data suggested that DNA methylation profiles of T cells and peripheral blood mononuclear cells (PB...
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12885-018-4482-7 |
| _version_ | 1818308013584285696 |
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| author | Surabhi Parashar David Cheishvili Niaz Mahmood Ani Arakelian Imrana Tanvir Haseeb Ahmed Khan Richard Kremer Catalin Mihalcioiu Moshe Szyf Shafaat A. Rabbani |
| author_facet | Surabhi Parashar David Cheishvili Niaz Mahmood Ani Arakelian Imrana Tanvir Haseeb Ahmed Khan Richard Kremer Catalin Mihalcioiu Moshe Szyf Shafaat A. Rabbani |
| author_sort | Surabhi Parashar |
| collection | DOAJ |
| description | Abstract Background Immune surveillance acts as a defense mechanism in cancer, and its disruption is involved in cancer progression. DNA methylation reflects the phenotypic identity of cells and recent data suggested that DNA methylation profiles of T cells and peripheral blood mononuclear cells (PBMC) are altered in cancer progression. Methods We enrolled 19 females with stage 1 and 2, nine with stage 3 and 4 and 9 age matched healthy women. T cells were isolated from peripheral blood and extracted DNA was subjected to Illumina 450 K DNA methylation array analysis. Raw data was analyzed by BMIQ, ChAMP and ComBat followed by validation of identified genes by pyrosequencing. Results Analysis of data revealed ~ 10,000 sites that correlated with breast cancer progression and established a list of 89 CG sites that were highly correlated (p < 0.01, r > 0.7, r < − 0.7) with breast cancer progression. The vast majority of these sites were hypomethylated and enriched in genes with functions in the immune system. Conclusions The study points to the possibility of using DNA methylation signatures as a noninvasive method for early detection of breast cancer and its progression which need to be tested in clinical studies. |
| first_indexed | 2024-12-13T07:07:31Z |
| format | Article |
| id | doaj.art-04072641a1564a9793a9c8b976a62e1b |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-13T07:07:31Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-04072641a1564a9793a9c8b976a62e1b2022-12-21T23:55:46ZengBMCBMC Cancer1471-24072018-05-011811910.1186/s12885-018-4482-7DNA methylation signatures of breast cancer in peripheral T-cellsSurabhi Parashar0David Cheishvili1Niaz Mahmood2Ani Arakelian3Imrana Tanvir4Haseeb Ahmed Khan5Richard Kremer6Catalin Mihalcioiu7Moshe Szyf8Shafaat A. Rabbani9Department of Medicine, McGill University Health CenterDepartment of Pharmacology and Therapeutics, McGill University Health CenterDepartment of Medicine, McGill University Health CenterDepartment of Medicine, McGill University Health CenterFatima Memorial HospitalFatima Memorial HospitalDepartment of Medicine, McGill University Health CenterDepartment of Medicine, McGill University Health CenterDepartment of Pharmacology and Therapeutics, McGill University Health CenterDepartment of Medicine, McGill University Health CenterAbstract Background Immune surveillance acts as a defense mechanism in cancer, and its disruption is involved in cancer progression. DNA methylation reflects the phenotypic identity of cells and recent data suggested that DNA methylation profiles of T cells and peripheral blood mononuclear cells (PBMC) are altered in cancer progression. Methods We enrolled 19 females with stage 1 and 2, nine with stage 3 and 4 and 9 age matched healthy women. T cells were isolated from peripheral blood and extracted DNA was subjected to Illumina 450 K DNA methylation array analysis. Raw data was analyzed by BMIQ, ChAMP and ComBat followed by validation of identified genes by pyrosequencing. Results Analysis of data revealed ~ 10,000 sites that correlated with breast cancer progression and established a list of 89 CG sites that were highly correlated (p < 0.01, r > 0.7, r < − 0.7) with breast cancer progression. The vast majority of these sites were hypomethylated and enriched in genes with functions in the immune system. Conclusions The study points to the possibility of using DNA methylation signatures as a noninvasive method for early detection of breast cancer and its progression which need to be tested in clinical studies.http://link.springer.com/article/10.1186/s12885-018-4482-7Breast cancerBiomarkersImmune systemDNA methylationBlood DNAEpigenetic signature |
| spellingShingle | Surabhi Parashar David Cheishvili Niaz Mahmood Ani Arakelian Imrana Tanvir Haseeb Ahmed Khan Richard Kremer Catalin Mihalcioiu Moshe Szyf Shafaat A. Rabbani DNA methylation signatures of breast cancer in peripheral T-cells BMC Cancer Breast cancer Biomarkers Immune system DNA methylation Blood DNA Epigenetic signature |
| title | DNA methylation signatures of breast cancer in peripheral T-cells |
| title_full | DNA methylation signatures of breast cancer in peripheral T-cells |
| title_fullStr | DNA methylation signatures of breast cancer in peripheral T-cells |
| title_full_unstemmed | DNA methylation signatures of breast cancer in peripheral T-cells |
| title_short | DNA methylation signatures of breast cancer in peripheral T-cells |
| title_sort | dna methylation signatures of breast cancer in peripheral t cells |
| topic | Breast cancer Biomarkers Immune system DNA methylation Blood DNA Epigenetic signature |
| url | http://link.springer.com/article/10.1186/s12885-018-4482-7 |
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