Identification and phylogenetic analysis of heme synthesis genes in trypanosomatids and their bacterial endosymbionts.
It has been known for decades that some insect-infecting trypanosomatids can survive in culture without heme supplementation while others cannot, and that this capability is associated with the presence of a betaproteobacterial endosymbiont in the flagellate's cytoplasm. However, the specific m...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21853145/?tool=EBI |
| _version_ | 1818401500356935680 |
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| author | João M P Alves Logan Voegtly Andrey V Matveyev Ana M Lara Flávia Maia da Silva Myrna G Serrano Gregory A Buck Marta M G Teixeira Erney P Camargo |
| author_facet | João M P Alves Logan Voegtly Andrey V Matveyev Ana M Lara Flávia Maia da Silva Myrna G Serrano Gregory A Buck Marta M G Teixeira Erney P Camargo |
| author_sort | João M P Alves |
| collection | DOAJ |
| description | It has been known for decades that some insect-infecting trypanosomatids can survive in culture without heme supplementation while others cannot, and that this capability is associated with the presence of a betaproteobacterial endosymbiont in the flagellate's cytoplasm. However, the specific mechanisms involved in this process remained obscure. In this work, we sequence and phylogenetically analyze the heme pathway genes from the symbionts and from their hosts, as well as from a number of heme synthesis-deficient Kinetoplastida. Our results show that the enzymes responsible for synthesis of heme are encoded on the symbiont genomes and produced in close cooperation with the flagellate host. Our evidence suggests that this synergistic relationship is the end result of a history of extensive gene loss and multiple lateral gene transfer events in different branches of the phylogeny of the Trypanosomatidae. |
| first_indexed | 2024-12-14T07:53:27Z |
| format | Article |
| id | doaj.art-04085939708e41d0af54a7c9f7372c78 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T07:53:27Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-04085939708e41d0af54a7c9f7372c782022-12-21T23:10:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2351810.1371/journal.pone.0023518Identification and phylogenetic analysis of heme synthesis genes in trypanosomatids and their bacterial endosymbionts.João M P AlvesLogan VoegtlyAndrey V MatveyevAna M LaraFlávia Maia da SilvaMyrna G SerranoGregory A BuckMarta M G TeixeiraErney P CamargoIt has been known for decades that some insect-infecting trypanosomatids can survive in culture without heme supplementation while others cannot, and that this capability is associated with the presence of a betaproteobacterial endosymbiont in the flagellate's cytoplasm. However, the specific mechanisms involved in this process remained obscure. In this work, we sequence and phylogenetically analyze the heme pathway genes from the symbionts and from their hosts, as well as from a number of heme synthesis-deficient Kinetoplastida. Our results show that the enzymes responsible for synthesis of heme are encoded on the symbiont genomes and produced in close cooperation with the flagellate host. Our evidence suggests that this synergistic relationship is the end result of a history of extensive gene loss and multiple lateral gene transfer events in different branches of the phylogeny of the Trypanosomatidae.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21853145/?tool=EBI |
| spellingShingle | João M P Alves Logan Voegtly Andrey V Matveyev Ana M Lara Flávia Maia da Silva Myrna G Serrano Gregory A Buck Marta M G Teixeira Erney P Camargo Identification and phylogenetic analysis of heme synthesis genes in trypanosomatids and their bacterial endosymbionts. PLoS ONE |
| title | Identification and phylogenetic analysis of heme synthesis genes in trypanosomatids and their bacterial endosymbionts. |
| title_full | Identification and phylogenetic analysis of heme synthesis genes in trypanosomatids and their bacterial endosymbionts. |
| title_fullStr | Identification and phylogenetic analysis of heme synthesis genes in trypanosomatids and their bacterial endosymbionts. |
| title_full_unstemmed | Identification and phylogenetic analysis of heme synthesis genes in trypanosomatids and their bacterial endosymbionts. |
| title_short | Identification and phylogenetic analysis of heme synthesis genes in trypanosomatids and their bacterial endosymbionts. |
| title_sort | identification and phylogenetic analysis of heme synthesis genes in trypanosomatids and their bacterial endosymbionts |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21853145/?tool=EBI |
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