Improvement of Drought Tolerance and Grain Yield in Common Bean by Overexpressing Trehalose-6-Phosphate Synthase in Rhizobia
Improving stress tolerance and yield in crops are major goals for agriculture. Here, we show a new strategy to increase drought tolerance and yield in legumes by overexpressing trehalose-6-phosphate synthase in the symbiotic bacterium Rhizobium etli. Phaseolus vulgaris (common beans) plants inoculat...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2008-07-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-7-0958 |
| _version_ | 1819045882264289280 |
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| author | Ramón Suárez Arnoldo Wong Mario Ramírez Aarón Barraza María del Carmen Orozco Miguel A. Cevallos Miguel Lara Georgina Hernández Gabriel Iturriaga |
| author_facet | Ramón Suárez Arnoldo Wong Mario Ramírez Aarón Barraza María del Carmen Orozco Miguel A. Cevallos Miguel Lara Georgina Hernández Gabriel Iturriaga |
| author_sort | Ramón Suárez |
| collection | DOAJ |
| description | Improving stress tolerance and yield in crops are major goals for agriculture. Here, we show a new strategy to increase drought tolerance and yield in legumes by overexpressing trehalose-6-phosphate synthase in the symbiotic bacterium Rhizobium etli. Phaseolus vulgaris (common beans) plants inoculated with R. etli overexpressing trehalose-6-phosphate synthase gene had more nodules with increased nitrogenase activity and higher biomass compared with plants inoculated with wild-type R. etli. In contrast, plants inoculated with an R. etli mutant in trehalose-6-phosphate synthase gene had fewer nodules and less nitrogenase activity and biomass. Three-week-old plants subjected to drought stress fully recovered whereas plants inoculated with a wild-type or mutant strain wilted and died. The yield of bean plants inoculated with R. etli overexpressing trehalose-6-phosphate synthase gene and grown with constant irrigation increased more than 50%. Macroarray analysis of 7,200 expressed sequence tags from nodules of plants inoculated with the strain overexpressing trehalose-6-phosphate synthase gene revealed upregulation of genes involved in stress tolerance and carbon and nitrogen metabolism, suggesting a signaling mechanism for trehalose. Thus, trehalose metabolism in rhizobia is key for signaling plant growth, yield, and adaptation to abiotic stress, and its manipulation has a major agronomical impact on leguminous plants. |
| first_indexed | 2024-12-21T10:35:38Z |
| format | Article |
| id | doaj.art-040b4d120b9a4d0ab5f97115f6190726 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-21T10:35:38Z |
| publishDate | 2008-07-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-040b4d120b9a4d0ab5f97115f61907262022-12-21T19:07:04ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062008-07-0121795896610.1094/MPMI-21-7-0958Improvement of Drought Tolerance and Grain Yield in Common Bean by Overexpressing Trehalose-6-Phosphate Synthase in RhizobiaRamón SuárezArnoldo WongMario RamírezAarón BarrazaMaría del Carmen OrozcoMiguel A. CevallosMiguel LaraGeorgina HernándezGabriel IturriagaImproving stress tolerance and yield in crops are major goals for agriculture. Here, we show a new strategy to increase drought tolerance and yield in legumes by overexpressing trehalose-6-phosphate synthase in the symbiotic bacterium Rhizobium etli. Phaseolus vulgaris (common beans) plants inoculated with R. etli overexpressing trehalose-6-phosphate synthase gene had more nodules with increased nitrogenase activity and higher biomass compared with plants inoculated with wild-type R. etli. In contrast, plants inoculated with an R. etli mutant in trehalose-6-phosphate synthase gene had fewer nodules and less nitrogenase activity and biomass. Three-week-old plants subjected to drought stress fully recovered whereas plants inoculated with a wild-type or mutant strain wilted and died. The yield of bean plants inoculated with R. etli overexpressing trehalose-6-phosphate synthase gene and grown with constant irrigation increased more than 50%. Macroarray analysis of 7,200 expressed sequence tags from nodules of plants inoculated with the strain overexpressing trehalose-6-phosphate synthase gene revealed upregulation of genes involved in stress tolerance and carbon and nitrogen metabolism, suggesting a signaling mechanism for trehalose. Thus, trehalose metabolism in rhizobia is key for signaling plant growth, yield, and adaptation to abiotic stress, and its manipulation has a major agronomical impact on leguminous plants.https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-7-0958OtsAotsA–TPS |
| spellingShingle | Ramón Suárez Arnoldo Wong Mario Ramírez Aarón Barraza María del Carmen Orozco Miguel A. Cevallos Miguel Lara Georgina Hernández Gabriel Iturriaga Improvement of Drought Tolerance and Grain Yield in Common Bean by Overexpressing Trehalose-6-Phosphate Synthase in Rhizobia Molecular Plant-Microbe Interactions OtsA otsA– TPS |
| title | Improvement of Drought Tolerance and Grain Yield in Common Bean by Overexpressing Trehalose-6-Phosphate Synthase in Rhizobia |
| title_full | Improvement of Drought Tolerance and Grain Yield in Common Bean by Overexpressing Trehalose-6-Phosphate Synthase in Rhizobia |
| title_fullStr | Improvement of Drought Tolerance and Grain Yield in Common Bean by Overexpressing Trehalose-6-Phosphate Synthase in Rhizobia |
| title_full_unstemmed | Improvement of Drought Tolerance and Grain Yield in Common Bean by Overexpressing Trehalose-6-Phosphate Synthase in Rhizobia |
| title_short | Improvement of Drought Tolerance and Grain Yield in Common Bean by Overexpressing Trehalose-6-Phosphate Synthase in Rhizobia |
| title_sort | improvement of drought tolerance and grain yield in common bean by overexpressing trehalose 6 phosphate synthase in rhizobia |
| topic | OtsA otsA– TPS |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-7-0958 |
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