Multilocus sequence analysis of <it>Treponema denticola</it> strains of diverse origin
<p>Abstract</p> <p>Background</p> <p>The oral spirochete bacterium <it>Treponema denticola</it> is associated with both the incidence and severity of periodontal disease. Although the biological or phenotypic properties of a significant number of <it>T...
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BMC
2013-02-01
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| Series: | BMC Microbiology |
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| Online Access: | http://www.biomedcentral.com/1471-2180/13/24 |
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| author | Mo Sisu You Meng Su Yvonne CF Lacap-Bugler Donnabella C Huo Yong-biao Smith Gavin JD Leung W Keung Watt Rory M |
| author_facet | Mo Sisu You Meng Su Yvonne CF Lacap-Bugler Donnabella C Huo Yong-biao Smith Gavin JD Leung W Keung Watt Rory M |
| author_sort | Mo Sisu |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The oral spirochete bacterium <it>Treponema denticola</it> is associated with both the incidence and severity of periodontal disease. Although the biological or phenotypic properties of a significant number of <it>T</it>. <it>denticola</it> isolates have been reported in the literature, their genetic diversity or phylogeny has never been systematically investigated. Here, we describe a multilocus sequence analysis (MLSA) of 20 of the most highly studied reference strains and clinical isolates of <it>T</it>. <it>denticola</it>; which were originally isolated from subgingival plaque samples taken from subjects from China, Japan, the Netherlands, Canada and the USA.</p> <p>Results</p> <p>The sequences of the 16S ribosomal RNA gene, and 7 conserved protein-encoding genes (<it>flaA</it>, <it>recA</it>, <it>pyrH</it>, <it>ppnK</it>, <it>dnaN</it>, <it>era</it> and <it>radC</it>) were successfully determined for each strain. Sequence data was analyzed using a variety of bioinformatic and phylogenetic software tools. We found no evidence of positive selection or DNA recombination within the protein-encoding genes, where levels of intraspecific sequence polymorphism varied from 18.8% (<it>flaA</it>) to 8.9% (<it>dnaN</it>). Phylogenetic analysis of the concatenated protein-encoding gene sequence data (ca. 6,513 nucleotides for each strain) using Bayesian and maximum likelihood approaches indicated that the <it>T</it>. <it>denticola</it> strains were monophyletic, and formed 6 well-defined clades. All analyzed <it>T</it>. <it>denticola</it> strains appeared to have a genetic origin distinct from that of ‘<it>Treponema vincentii</it>’ or <it>Treponema pallidum</it>. No specific geographical relationships could be established; but several strains isolated from different continents appear to be closely related at the genetic level.</p> <p>Conclusions</p> <p>Our analyses indicate that previous biological and biophysical investigations have predominantly focused on a subset of <it>T</it>. <it>denticola</it> strains with a relatively narrow range of genetic diversity. Our methodology and results establish a genetic framework for the discrimination and phylogenetic analysis of <it>T</it>. <it>denticola</it> isolates, which will greatly assist future biological and epidemiological investigations involving this putative ‘periodontopathogen’.</p> |
| first_indexed | 2024-12-14T05:55:59Z |
| format | Article |
| id | doaj.art-040d6fa36cc74fcb9841c1ddd87d6e24 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-14T05:55:59Z |
| publishDate | 2013-02-01 |
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| series | BMC Microbiology |
| spelling | doaj.art-040d6fa36cc74fcb9841c1ddd87d6e242022-12-21T23:14:34ZengBMCBMC Microbiology1471-21802013-02-011312410.1186/1471-2180-13-24Multilocus sequence analysis of <it>Treponema denticola</it> strains of diverse originMo SisuYou MengSu Yvonne CFLacap-Bugler Donnabella CHuo Yong-biaoSmith Gavin JDLeung W KeungWatt Rory M<p>Abstract</p> <p>Background</p> <p>The oral spirochete bacterium <it>Treponema denticola</it> is associated with both the incidence and severity of periodontal disease. Although the biological or phenotypic properties of a significant number of <it>T</it>. <it>denticola</it> isolates have been reported in the literature, their genetic diversity or phylogeny has never been systematically investigated. Here, we describe a multilocus sequence analysis (MLSA) of 20 of the most highly studied reference strains and clinical isolates of <it>T</it>. <it>denticola</it>; which were originally isolated from subgingival plaque samples taken from subjects from China, Japan, the Netherlands, Canada and the USA.</p> <p>Results</p> <p>The sequences of the 16S ribosomal RNA gene, and 7 conserved protein-encoding genes (<it>flaA</it>, <it>recA</it>, <it>pyrH</it>, <it>ppnK</it>, <it>dnaN</it>, <it>era</it> and <it>radC</it>) were successfully determined for each strain. Sequence data was analyzed using a variety of bioinformatic and phylogenetic software tools. We found no evidence of positive selection or DNA recombination within the protein-encoding genes, where levels of intraspecific sequence polymorphism varied from 18.8% (<it>flaA</it>) to 8.9% (<it>dnaN</it>). Phylogenetic analysis of the concatenated protein-encoding gene sequence data (ca. 6,513 nucleotides for each strain) using Bayesian and maximum likelihood approaches indicated that the <it>T</it>. <it>denticola</it> strains were monophyletic, and formed 6 well-defined clades. All analyzed <it>T</it>. <it>denticola</it> strains appeared to have a genetic origin distinct from that of ‘<it>Treponema vincentii</it>’ or <it>Treponema pallidum</it>. No specific geographical relationships could be established; but several strains isolated from different continents appear to be closely related at the genetic level.</p> <p>Conclusions</p> <p>Our analyses indicate that previous biological and biophysical investigations have predominantly focused on a subset of <it>T</it>. <it>denticola</it> strains with a relatively narrow range of genetic diversity. Our methodology and results establish a genetic framework for the discrimination and phylogenetic analysis of <it>T</it>. <it>denticola</it> isolates, which will greatly assist future biological and epidemiological investigations involving this putative ‘periodontopathogen’.</p>http://www.biomedcentral.com/1471-2180/13/24<it>Treponema denticola</it>Periodontal diseasePhylogenyMultilocus sequence analysisMLSASpirocheteOral microbiotaInfectious diseasesDentistry |
| spellingShingle | Mo Sisu You Meng Su Yvonne CF Lacap-Bugler Donnabella C Huo Yong-biao Smith Gavin JD Leung W Keung Watt Rory M Multilocus sequence analysis of <it>Treponema denticola</it> strains of diverse origin BMC Microbiology <it>Treponema denticola</it> Periodontal disease Phylogeny Multilocus sequence analysis MLSA Spirochete Oral microbiota Infectious diseases Dentistry |
| title | Multilocus sequence analysis of <it>Treponema denticola</it> strains of diverse origin |
| title_full | Multilocus sequence analysis of <it>Treponema denticola</it> strains of diverse origin |
| title_fullStr | Multilocus sequence analysis of <it>Treponema denticola</it> strains of diverse origin |
| title_full_unstemmed | Multilocus sequence analysis of <it>Treponema denticola</it> strains of diverse origin |
| title_short | Multilocus sequence analysis of <it>Treponema denticola</it> strains of diverse origin |
| title_sort | multilocus sequence analysis of it treponema denticola it strains of diverse origin |
| topic | <it>Treponema denticola</it> Periodontal disease Phylogeny Multilocus sequence analysis MLSA Spirochete Oral microbiota Infectious diseases Dentistry |
| url | http://www.biomedcentral.com/1471-2180/13/24 |
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