Development and characterization of highly informative ELISA for the detection of IgG and IgA antibodies to Сhlamydia trachomatis
The goal of this work was developing of highly informative an enzyme-linked immunosorbent assay (ELISA) for the detection of IgG and IgA antibodies against to Chlamydia trachomatis, as well as comparative characterization of developed assay using standardized control materials. The study was conduct...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2018-04-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2018/04/Galkin_3_18.pdf |
| _version_ | 1797641726971609088 |
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| author | O. Yu. Galkin Yu. V. Gorshunov O. B. Besarab O. M. Ivanova |
| author_facet | O. Yu. Galkin Yu. V. Gorshunov O. B. Besarab O. M. Ivanova |
| author_sort | O. Yu. Galkin |
| collection | DOAJ |
| description | The goal of this work was developing of highly informative an enzyme-linked immunosorbent assay (ELISA) for the detection of IgG and IgA antibodies against to Chlamydia trachomatis, as well as comparative characterization of developed assay using standardized control materials. The study was conducted using: monoclonal antibodies (McAbs) to human IgA and IgG; recombinant Ch. trachomatis proteins – Pgp3, major outer membrane protein (MOMP); two panels of characterized sera and four reference ELISA kits. The study of immunochemical activity of peroxidase conjugates of McAbs was performed in comparison with conjugates of commercial analogues: anti-IgG McAb 2A11 and anti-IgA McAb AD3. About half of the conjugates from the received McAbs panel were more active compared to the reference antibody conjugates. It was quite justified to use the conjugates of antibodies that interact with different antigenic determinants. When IgG antibodies were detected to MOMP, it was justified 1.14-1.56 times more; when IgA antibodies were detected to MOMP, it was justified 1.16-1.37 times more. ELISA for detecting IgG/IgA antibodies to MOMP and Pgp3 of Ch. trachomatis were evaluated using appropriately described serum panels OCO-42-28-313-00 and OCO-42-28-314-00. Comparative studies of the developed ELISA for the detection of IgG and IgA antibodies to the MOMP and Pgp3 of Ch. trachomatis showed their prominent advantage over the commercial analogues, which more clearly demonstrates the difference in the ratio of average values of optical density of positive and negative samples of the described panel of sera: this indicator for commercial kits was 1.36-3.59 times less. |
| first_indexed | 2024-03-11T13:49:48Z |
| format | Article |
| id | doaj.art-0414b464d7b049baa329403af3817c3f |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T13:49:48Z |
| publishDate | 2018-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-0414b464d7b049baa329403af3817c3f2023-11-02T09:08:35ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032018-04-01903708310.15407/ubj90.03.070Development and characterization of highly informative ELISA for the detection of IgG and IgA antibodies to Сhlamydia trachomatisO. Yu. Galkin0Yu. V. Gorshunov1O. B. Besarab2O. M. Ivanova3National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”Xema Ltd., KyivThe goal of this work was developing of highly informative an enzyme-linked immunosorbent assay (ELISA) for the detection of IgG and IgA antibodies against to Chlamydia trachomatis, as well as comparative characterization of developed assay using standardized control materials. The study was conducted using: monoclonal antibodies (McAbs) to human IgA and IgG; recombinant Ch. trachomatis proteins – Pgp3, major outer membrane protein (MOMP); two panels of characterized sera and four reference ELISA kits. The study of immunochemical activity of peroxidase conjugates of McAbs was performed in comparison with conjugates of commercial analogues: anti-IgG McAb 2A11 and anti-IgA McAb AD3. About half of the conjugates from the received McAbs panel were more active compared to the reference antibody conjugates. It was quite justified to use the conjugates of antibodies that interact with different antigenic determinants. When IgG antibodies were detected to MOMP, it was justified 1.14-1.56 times more; when IgA antibodies were detected to MOMP, it was justified 1.16-1.37 times more. ELISA for detecting IgG/IgA antibodies to MOMP and Pgp3 of Ch. trachomatis were evaluated using appropriately described serum panels OCO-42-28-313-00 and OCO-42-28-314-00. Comparative studies of the developed ELISA for the detection of IgG and IgA antibodies to the MOMP and Pgp3 of Ch. trachomatis showed their prominent advantage over the commercial analogues, which more clearly demonstrates the difference in the ratio of average values of optical density of positive and negative samples of the described panel of sera: this indicator for commercial kits was 1.36-3.59 times less.http://ukrbiochemjournal.org/wp-content/uploads/2018/04/Galkin_3_18.pdfChlamydia trachomatisenzyme-linked immunosorbent assaymajor outer membrane proteinPgp3sensitivityspecificity |
| spellingShingle | O. Yu. Galkin Yu. V. Gorshunov O. B. Besarab O. M. Ivanova Development and characterization of highly informative ELISA for the detection of IgG and IgA antibodies to Сhlamydia trachomatis The Ukrainian Biochemical Journal Chlamydia trachomatis enzyme-linked immunosorbent assay major outer membrane protein Pgp3 sensitivity specificity |
| title | Development and characterization of highly informative ELISA for the detection of IgG and IgA antibodies to Сhlamydia trachomatis |
| title_full | Development and characterization of highly informative ELISA for the detection of IgG and IgA antibodies to Сhlamydia trachomatis |
| title_fullStr | Development and characterization of highly informative ELISA for the detection of IgG and IgA antibodies to Сhlamydia trachomatis |
| title_full_unstemmed | Development and characterization of highly informative ELISA for the detection of IgG and IgA antibodies to Сhlamydia trachomatis |
| title_short | Development and characterization of highly informative ELISA for the detection of IgG and IgA antibodies to Сhlamydia trachomatis |
| title_sort | development and characterization of highly informative elisa for the detection of igg and iga antibodies to сhlamydia trachomatis |
| topic | Chlamydia trachomatis enzyme-linked immunosorbent assay major outer membrane protein Pgp3 sensitivity specificity |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2018/04/Galkin_3_18.pdf |
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