Differential metabolic markers associated with primary open-angle glaucoma and cataract in human aqueous humor
Abstract Background We aimed to identify metabolic biomarkers and investigate the metabolic alterations in relation to primary open-angle glaucoma (POAG) and cataract in human aqueous humor. Methods Sixteen POAG patients undergoing surgical treatments and 24 patients undergoing cataract surgeries we...
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| Format: | Article |
| Language: | English |
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BMC
2020-05-01
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| Series: | BMC Ophthalmology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12886-020-01452-7 |
| _version_ | 1818127571107184640 |
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| author | Chen-Wei Pan Chaofu Ke Qin Chen Yi-Jin Tao Xu Zha Yuan-Ping Zhang Hua Zhong |
| author_facet | Chen-Wei Pan Chaofu Ke Qin Chen Yi-Jin Tao Xu Zha Yuan-Ping Zhang Hua Zhong |
| author_sort | Chen-Wei Pan |
| collection | DOAJ |
| description | Abstract Background We aimed to identify metabolic biomarkers and investigate the metabolic alterations in relation to primary open-angle glaucoma (POAG) and cataract in human aqueous humor. Methods Sixteen POAG patients undergoing surgical treatments and 24 patients undergoing cataract surgeries were included in this case-control study. We performed the metabolomic analysis of aqueous humor samples using a non-targeted gas chromatography coupled to time-of-flight mass spectrometer. The area under the receiver operating characteristic curve (AUC) was computed to assess the discrimination capacities of each metabolite marker. Databases including the Kyoto Encyclopedia of Genes and Genomes (KEGG) and MetaboAnalyst were utilized to search for the potential pathways of metabolites. Results Aqueous humor metabolomic profiles could well distinguish POAG from controls. Fourteen metabolic biomarkers were identified as potential aqueous humor biomarkers for POAG, yielding AUC values from 0.62 to 0.86. In pathway analysis, Biotin metabolism was highly impacted, implying that these metabolic markers play important roles in the regulation of this pathway. Conclusions This study identified valuable metabolic biomarkers and pathways that may facilitate an improved understanding of the POAG pathogenesis. The finding holds translational value in the development of new therapeutic measures for POAG. |
| first_indexed | 2024-12-11T07:19:28Z |
| format | Article |
| id | doaj.art-0414eb05a46b4f1abd48b2254ea89f21 |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-12-11T07:19:28Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-0414eb05a46b4f1abd48b2254ea89f212022-12-22T01:16:07ZengBMCBMC Ophthalmology1471-24152020-05-012011810.1186/s12886-020-01452-7Differential metabolic markers associated with primary open-angle glaucoma and cataract in human aqueous humorChen-Wei Pan0Chaofu Ke1Qin Chen2Yi-Jin Tao3Xu Zha4Yuan-Ping Zhang5Hua Zhong6School of Public Health, Medical College of Soochow UniversitySchool of Public Health, Medical College of Soochow UniversityDepartment of Ophthalmology, the First Affiliated Hospital with Nanjing Medical UniversityDepartment of Ophthalmology, the First Affiliated Hospital of Kunming Medical UniversityDepartment of Ophthalmology, the Second Affiliated Hospital of Kunming Medical UniversityDepartment of Ophthalmology, the Second Affiliated Hospital of Kunming Medical UniversityDepartment of Ophthalmology, the First Affiliated Hospital of Kunming Medical UniversityAbstract Background We aimed to identify metabolic biomarkers and investigate the metabolic alterations in relation to primary open-angle glaucoma (POAG) and cataract in human aqueous humor. Methods Sixteen POAG patients undergoing surgical treatments and 24 patients undergoing cataract surgeries were included in this case-control study. We performed the metabolomic analysis of aqueous humor samples using a non-targeted gas chromatography coupled to time-of-flight mass spectrometer. The area under the receiver operating characteristic curve (AUC) was computed to assess the discrimination capacities of each metabolite marker. Databases including the Kyoto Encyclopedia of Genes and Genomes (KEGG) and MetaboAnalyst were utilized to search for the potential pathways of metabolites. Results Aqueous humor metabolomic profiles could well distinguish POAG from controls. Fourteen metabolic biomarkers were identified as potential aqueous humor biomarkers for POAG, yielding AUC values from 0.62 to 0.86. In pathway analysis, Biotin metabolism was highly impacted, implying that these metabolic markers play important roles in the regulation of this pathway. Conclusions This study identified valuable metabolic biomarkers and pathways that may facilitate an improved understanding of the POAG pathogenesis. The finding holds translational value in the development of new therapeutic measures for POAG.http://link.springer.com/article/10.1186/s12886-020-01452-7Open-angle glaucomaCataractEpidemiologyMetabolomics |
| spellingShingle | Chen-Wei Pan Chaofu Ke Qin Chen Yi-Jin Tao Xu Zha Yuan-Ping Zhang Hua Zhong Differential metabolic markers associated with primary open-angle glaucoma and cataract in human aqueous humor BMC Ophthalmology Open-angle glaucoma Cataract Epidemiology Metabolomics |
| title | Differential metabolic markers associated with primary open-angle glaucoma and cataract in human aqueous humor |
| title_full | Differential metabolic markers associated with primary open-angle glaucoma and cataract in human aqueous humor |
| title_fullStr | Differential metabolic markers associated with primary open-angle glaucoma and cataract in human aqueous humor |
| title_full_unstemmed | Differential metabolic markers associated with primary open-angle glaucoma and cataract in human aqueous humor |
| title_short | Differential metabolic markers associated with primary open-angle glaucoma and cataract in human aqueous humor |
| title_sort | differential metabolic markers associated with primary open angle glaucoma and cataract in human aqueous humor |
| topic | Open-angle glaucoma Cataract Epidemiology Metabolomics |
| url | http://link.springer.com/article/10.1186/s12886-020-01452-7 |
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