Comparative Studies on Essential Oil and Phenolic Content with In Vitro Antioxidant, Anticholinesterase, Antimicrobial Activities of <i>Achillea biebersteinii</i> Afan. and <i>A. millefolium</i> subsp. <i>millefolium</i> Afan. L. Growing in Eastern Turkey
The potential applications of <i>Achillea</i> species in various industries have encouraged the examination of their phytochemical components along with their biological potential. In the present study, phenolic contents and essential oil compositions together with the in vitro biologica...
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MDPI AG
2022-03-01
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| author | Gizem Gülsoy Toplan Turgut Taşkın Gökalp İşcan Fatih Göger Mine Kürkçüoğlu Ayşe Civaş Gülay Ecevit-Genç Afife Mat Kemal Hüsnü Can Başer |
| author_facet | Gizem Gülsoy Toplan Turgut Taşkın Gökalp İşcan Fatih Göger Mine Kürkçüoğlu Ayşe Civaş Gülay Ecevit-Genç Afife Mat Kemal Hüsnü Can Başer |
| author_sort | Gizem Gülsoy Toplan |
| collection | DOAJ |
| description | The potential applications of <i>Achillea</i> species in various industries have encouraged the examination of their phytochemical components along with their biological potential. In the present study, phenolic contents and essential oil compositions together with the in vitro biological activities of the aerial parts from <i>Achillea biebersteinii</i> Afan. and <i>Achillea millefolium</i> subsp. <i>millefolium</i> Afan. collected from Turkey were evaluated. Different solvent extracts (<i>n</i>-hexane, chloroform, methanol, water) were prepared and their antimicrobial, anticholinesterase, and antioxidant activities were studied. The LC-MS/MS results revealed the presence of 16 different phenolic compounds, including chlorogenic acid, rutin, quercetin, and luteolin glycosides, in methanolic extracts. According to GC-FID and GC/MS results, the primary components of the oils were identified as 1,8-cineole (32.5%), piperitone (14.4%), and camphor (13.7%) in <i>A. biebersteinii</i> and 1,8-cineole (12.3%) and β-eudesmol (8.9%) in <i>A. millefolium</i> subsp. <i>millefolium</i>. The infusion and methanolic extracts of both species were found to be rich in their total phenolic content as well as their antioxidant and anticholinesterase activity. In contrast, the <i>n</i>-hexane and chloroform extracts of both species showed strong antimicrobial activity with MIC values ranging from 15 to 2000 μg/mL. Our findings suggest that the investigated <i>Achillea</i> species could be evaluated as potent natural agents, and further studies into the promising extracts are needed. |
| first_indexed | 2024-03-09T13:09:14Z |
| format | Article |
| id | doaj.art-0415fe100ae54a03bd668193b55682ed |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T13:09:14Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0415fe100ae54a03bd668193b55682ed2023-11-30T21:43:47ZengMDPI AGMolecules1420-30492022-03-01276195610.3390/molecules27061956Comparative Studies on Essential Oil and Phenolic Content with In Vitro Antioxidant, Anticholinesterase, Antimicrobial Activities of <i>Achillea biebersteinii</i> Afan. and <i>A. millefolium</i> subsp. <i>millefolium</i> Afan. L. Growing in Eastern TurkeyGizem Gülsoy Toplan0Turgut Taşkın1Gökalp İşcan2Fatih Göger3Mine Kürkçüoğlu4Ayşe Civaş5Gülay Ecevit-Genç6Afife Mat7Kemal Hüsnü Can Başer8Department of Pharmacognosy, Faculty of Pharmacy, Istinye University, Istanbul 34010, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Marmara University, Istanbul 34668, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir 26470, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir 26470, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir 26470, TurkeyDepartment of Pharmacy and Pharmaceutical Services, Tuzluca Vocational School, Igdır University, Igdir 76000, TurkeyDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Istanbul University, Istanbul 34116, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Istanbul University, Istanbul 34116, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Near East University, Nicosia 99138, CyprusThe potential applications of <i>Achillea</i> species in various industries have encouraged the examination of their phytochemical components along with their biological potential. In the present study, phenolic contents and essential oil compositions together with the in vitro biological activities of the aerial parts from <i>Achillea biebersteinii</i> Afan. and <i>Achillea millefolium</i> subsp. <i>millefolium</i> Afan. collected from Turkey were evaluated. Different solvent extracts (<i>n</i>-hexane, chloroform, methanol, water) were prepared and their antimicrobial, anticholinesterase, and antioxidant activities were studied. The LC-MS/MS results revealed the presence of 16 different phenolic compounds, including chlorogenic acid, rutin, quercetin, and luteolin glycosides, in methanolic extracts. According to GC-FID and GC/MS results, the primary components of the oils were identified as 1,8-cineole (32.5%), piperitone (14.4%), and camphor (13.7%) in <i>A. biebersteinii</i> and 1,8-cineole (12.3%) and β-eudesmol (8.9%) in <i>A. millefolium</i> subsp. <i>millefolium</i>. The infusion and methanolic extracts of both species were found to be rich in their total phenolic content as well as their antioxidant and anticholinesterase activity. In contrast, the <i>n</i>-hexane and chloroform extracts of both species showed strong antimicrobial activity with MIC values ranging from 15 to 2000 μg/mL. Our findings suggest that the investigated <i>Achillea</i> species could be evaluated as potent natural agents, and further studies into the promising extracts are needed.https://www.mdpi.com/1420-3049/27/6/1956<i>Achillea biebersteinii</i><i>Achillea millefolium</i> subsp. <i>millefolium</i>LC-MS/MSessential oilGC-GC/MSantimicrobial |
| spellingShingle | Gizem Gülsoy Toplan Turgut Taşkın Gökalp İşcan Fatih Göger Mine Kürkçüoğlu Ayşe Civaş Gülay Ecevit-Genç Afife Mat Kemal Hüsnü Can Başer Comparative Studies on Essential Oil and Phenolic Content with In Vitro Antioxidant, Anticholinesterase, Antimicrobial Activities of <i>Achillea biebersteinii</i> Afan. and <i>A. millefolium</i> subsp. <i>millefolium</i> Afan. L. Growing in Eastern Turkey Molecules <i>Achillea biebersteinii</i> <i>Achillea millefolium</i> subsp. <i>millefolium</i> LC-MS/MS essential oil GC-GC/MS antimicrobial |
| title | Comparative Studies on Essential Oil and Phenolic Content with In Vitro Antioxidant, Anticholinesterase, Antimicrobial Activities of <i>Achillea biebersteinii</i> Afan. and <i>A. millefolium</i> subsp. <i>millefolium</i> Afan. L. Growing in Eastern Turkey |
| title_full | Comparative Studies on Essential Oil and Phenolic Content with In Vitro Antioxidant, Anticholinesterase, Antimicrobial Activities of <i>Achillea biebersteinii</i> Afan. and <i>A. millefolium</i> subsp. <i>millefolium</i> Afan. L. Growing in Eastern Turkey |
| title_fullStr | Comparative Studies on Essential Oil and Phenolic Content with In Vitro Antioxidant, Anticholinesterase, Antimicrobial Activities of <i>Achillea biebersteinii</i> Afan. and <i>A. millefolium</i> subsp. <i>millefolium</i> Afan. L. Growing in Eastern Turkey |
| title_full_unstemmed | Comparative Studies on Essential Oil and Phenolic Content with In Vitro Antioxidant, Anticholinesterase, Antimicrobial Activities of <i>Achillea biebersteinii</i> Afan. and <i>A. millefolium</i> subsp. <i>millefolium</i> Afan. L. Growing in Eastern Turkey |
| title_short | Comparative Studies on Essential Oil and Phenolic Content with In Vitro Antioxidant, Anticholinesterase, Antimicrobial Activities of <i>Achillea biebersteinii</i> Afan. and <i>A. millefolium</i> subsp. <i>millefolium</i> Afan. L. Growing in Eastern Turkey |
| title_sort | comparative studies on essential oil and phenolic content with in vitro antioxidant anticholinesterase antimicrobial activities of i achillea biebersteinii i afan and i a millefolium i subsp i millefolium i afan l growing in eastern turkey |
| topic | <i>Achillea biebersteinii</i> <i>Achillea millefolium</i> subsp. <i>millefolium</i> LC-MS/MS essential oil GC-GC/MS antimicrobial |
| url | https://www.mdpi.com/1420-3049/27/6/1956 |
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