Preparation of Compound Selenium-enriched Plant Solid Beverage and Its Biological Activities
A functional compound selenium-enriched plant solid beverage was successfully developed by taking the selenium-enriched medicinal and edible plants Brassica oleracea, Pueraria lobata, Ampelopsis grossedentata, Polygonatum sibiricum, Chaenomeles sinensis, and tomato as the materials. At the meantime,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-12-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023020216 |
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| author | Guijuan ZHENG Jianqun LUO Zhandong NAN Min YANG Meijun HE Guangmin YAO |
| author_facet | Guijuan ZHENG Jianqun LUO Zhandong NAN Min YANG Meijun HE Guangmin YAO |
| author_sort | Guijuan ZHENG |
| collection | DOAJ |
| description | A functional compound selenium-enriched plant solid beverage was successfully developed by taking the selenium-enriched medicinal and edible plants Brassica oleracea, Pueraria lobata, Ampelopsis grossedentata, Polygonatum sibiricum, Chaenomeles sinensis, and tomato as the materials. At the meantime, its biological activities including antiproliferative, antioxidant, and analgesic activities were evaluated. The total selenium content of the compound solid beverage was determined by fluorescence spectrophotometry as 540±30.12 μg/kg. The biological results showed that the compound selenium-enriched plant solid beverage inhibited the proliferation of four cancerous cell lines A549, SMMC-7721, MCF-7, and SW480 with the inhibition values of 4.09%±1.36%, 19.48%±3.18%, 30.75%±1.45%, and 18.20%±1.12%, respectively, and had no cytotoxicity to the human normal lung epithelial cell BEAS-2B at a concentration of 100 μg/mL, indicating its antiproliferative effect against cancer cells in vitro. The compound solid beverage had a clearance ability of DPPH radical at a concentration-dependent manner with an IC50 value of 435.6±27.2 μg/mL. The compound solid beverage reduced the H2O2-induced oxidative damage and the intracellular reactive oxygen fluorescence intensity in LO2 cells, and presented a dose-effect relationship within a concentration of 5~20 μg/mL, revealing its obvious antioxidant activity (P<0.01). Additionally, the compound plant selenium solid beverage exhibited significant analgesic activity at the high-dose of 250 mg/kg with an inhibition rate of 92.3% (P<0.001), which was equivalent to that of the positive drug morphine (5 mg/kg). The research results could lay a foundation for further development of the compound selenium-rich plant solid drink. |
| first_indexed | 2024-03-09T01:10:16Z |
| format | Article |
| id | doaj.art-041619c0d8454df99c5555570ef6e461 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-09T01:10:16Z |
| publishDate | 2023-12-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-041619c0d8454df99c5555570ef6e4612023-12-11T05:43:27ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-12-01442434134810.13386/j.issn1002-0306.20230202162023020216-24Preparation of Compound Selenium-enriched Plant Solid Beverage and Its Biological ActivitiesGuijuan ZHENG0Jianqun LUO1Zhandong NAN2Min YANG3Meijun HE4Guangmin YAO5School of Pharmacy, Tongji Medical College, Huazhong University of Science of Technology, Wuhan 430030, ChinaEnshi Xihe Biotechnology Co., Ltd., Enshi 445000, ChinaEnshi Xihe Biotechnology Co., Ltd., Enshi 445000, ChinaEnshi Xihe Biotechnology Co., Ltd., Enshi 445000, ChinaEnshi Xihe Biotechnology Co., Ltd., Enshi 445000, ChinaSchool of Pharmacy, Tongji Medical College, Huazhong University of Science of Technology, Wuhan 430030, ChinaA functional compound selenium-enriched plant solid beverage was successfully developed by taking the selenium-enriched medicinal and edible plants Brassica oleracea, Pueraria lobata, Ampelopsis grossedentata, Polygonatum sibiricum, Chaenomeles sinensis, and tomato as the materials. At the meantime, its biological activities including antiproliferative, antioxidant, and analgesic activities were evaluated. The total selenium content of the compound solid beverage was determined by fluorescence spectrophotometry as 540±30.12 μg/kg. The biological results showed that the compound selenium-enriched plant solid beverage inhibited the proliferation of four cancerous cell lines A549, SMMC-7721, MCF-7, and SW480 with the inhibition values of 4.09%±1.36%, 19.48%±3.18%, 30.75%±1.45%, and 18.20%±1.12%, respectively, and had no cytotoxicity to the human normal lung epithelial cell BEAS-2B at a concentration of 100 μg/mL, indicating its antiproliferative effect against cancer cells in vitro. The compound solid beverage had a clearance ability of DPPH radical at a concentration-dependent manner with an IC50 value of 435.6±27.2 μg/mL. The compound solid beverage reduced the H2O2-induced oxidative damage and the intracellular reactive oxygen fluorescence intensity in LO2 cells, and presented a dose-effect relationship within a concentration of 5~20 μg/mL, revealing its obvious antioxidant activity (P<0.01). Additionally, the compound plant selenium solid beverage exhibited significant analgesic activity at the high-dose of 250 mg/kg with an inhibition rate of 92.3% (P<0.001), which was equivalent to that of the positive drug morphine (5 mg/kg). The research results could lay a foundation for further development of the compound selenium-rich plant solid drink.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023020216compound selenium-enriched plant solid beveragebiological evaluationantiproliferative activityantioxidant activityanalgesic activity |
| spellingShingle | Guijuan ZHENG Jianqun LUO Zhandong NAN Min YANG Meijun HE Guangmin YAO Preparation of Compound Selenium-enriched Plant Solid Beverage and Its Biological Activities Shipin gongye ke-ji compound selenium-enriched plant solid beverage biological evaluation antiproliferative activity antioxidant activity analgesic activity |
| title | Preparation of Compound Selenium-enriched Plant Solid Beverage and Its Biological Activities |
| title_full | Preparation of Compound Selenium-enriched Plant Solid Beverage and Its Biological Activities |
| title_fullStr | Preparation of Compound Selenium-enriched Plant Solid Beverage and Its Biological Activities |
| title_full_unstemmed | Preparation of Compound Selenium-enriched Plant Solid Beverage and Its Biological Activities |
| title_short | Preparation of Compound Selenium-enriched Plant Solid Beverage and Its Biological Activities |
| title_sort | preparation of compound selenium enriched plant solid beverage and its biological activities |
| topic | compound selenium-enriched plant solid beverage biological evaluation antiproliferative activity antioxidant activity analgesic activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023020216 |
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