A DNA barcoding approach to identify species of Nemacheilidae: a complex family of cypriniform fishes in Iran
Nemacheilian loaches are a group of freshwater fishes which are distributed in many inland waters of Iran. They are complex species from aspects of systematic and many species of this family are morphologically undistinguishable. Therefore, due to limitations behind the morphology-based identificati...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Ferdowsi University of Mashhad
2019-11-01
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| Series: | Iranian Journal of Animal Biosystematics |
| Subjects: | |
| Online Access: | https://ijab.um.ac.ir/article_30186_a8f3153e189718abee59115923c1512a.pdf |
| _version_ | 1811269783299031040 |
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| author | Hamed Paknejad Ali Jalali Davoud Fadakar Ghasem Askari Hamed Azadi |
| author_facet | Hamed Paknejad Ali Jalali Davoud Fadakar Ghasem Askari Hamed Azadi |
| author_sort | Hamed Paknejad |
| collection | DOAJ |
| description | Nemacheilian loaches are a group of freshwater fishes which are distributed in many inland waters of Iran. They are complex species from aspects of systematic and many species of this family are morphologically undistinguishable. Therefore, due to limitations behind the morphology-based identification of such species, genetic approaches such as DNA barcoding can be helpful to distinguish species, although their genetic relationships are not well determined yet. The present investigation provides data on genetic structure of some species of Nemachilidae including Paraschistura bampurensis, Oxynoemacheilus kiabii and Turcinemacheilus saadii from inland waters of Iran. These species were sequenced for a 652 base pair region of the mitochondrial cytochrome oxidase subunit I gene. All species were identified by cytochrome oxidase subunit I gene sequence. The sequences of P. bampurensis showed that specimens collected from two streams (Shapour and Fahlian, south-west of Iran), with 1.89% within-species Kimura two parameter distance, shared haplotype in neighbor joining tree. The results provided the first genetic evidence for O. kiabii, which has recently been recorded as a new species in Iran, and also cytochrome oxidase subunit I gene data showed that O. kiabii is completely different from other species of Nemacheilidae. The results suggested that cytochrome oxidase subunit I gene sequencing can be used to identify species of Nemachilidae from the Iranian streams. |
| first_indexed | 2024-04-12T21:49:18Z |
| format | Article |
| id | doaj.art-0419d27776504dfa8acdea91c10d693e |
| institution | Directory Open Access Journal |
| issn | 1735-434X 2423-4222 |
| language | English |
| last_indexed | 2024-04-12T21:49:18Z |
| publishDate | 2019-11-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | Iranian Journal of Animal Biosystematics |
| spelling | doaj.art-0419d27776504dfa8acdea91c10d693e2022-12-22T03:15:32ZengFerdowsi University of MashhadIranian Journal of Animal Biosystematics1735-434X2423-42222019-11-0115110.22067/ijab.v15i1.5832130186A DNA barcoding approach to identify species of Nemacheilidae: a complex family of cypriniform fishes in IranHamed Paknejad0Ali Jalali1Davoud Fadakar2Ghasem Askari3Hamed Azadi4Gorgan UniversityDeakin UniversityGorgan University of Agricultural Sciences and Natural ResourcesGorgan University of Agricultural Sciences and Natural ResourcesGorgan University of Agricultural Sciences and Natural ResourcesNemacheilian loaches are a group of freshwater fishes which are distributed in many inland waters of Iran. They are complex species from aspects of systematic and many species of this family are morphologically undistinguishable. Therefore, due to limitations behind the morphology-based identification of such species, genetic approaches such as DNA barcoding can be helpful to distinguish species, although their genetic relationships are not well determined yet. The present investigation provides data on genetic structure of some species of Nemachilidae including Paraschistura bampurensis, Oxynoemacheilus kiabii and Turcinemacheilus saadii from inland waters of Iran. These species were sequenced for a 652 base pair region of the mitochondrial cytochrome oxidase subunit I gene. All species were identified by cytochrome oxidase subunit I gene sequence. The sequences of P. bampurensis showed that specimens collected from two streams (Shapour and Fahlian, south-west of Iran), with 1.89% within-species Kimura two parameter distance, shared haplotype in neighbor joining tree. The results provided the first genetic evidence for O. kiabii, which has recently been recorded as a new species in Iran, and also cytochrome oxidase subunit I gene data showed that O. kiabii is completely different from other species of Nemacheilidae. The results suggested that cytochrome oxidase subunit I gene sequencing can be used to identify species of Nemachilidae from the Iranian streams.https://ijab.um.ac.ir/article_30186_a8f3153e189718abee59115923c1512a.pdfcytochrome oxidasedna barcodingnemachilidaephylogenetic treespecies identification |
| spellingShingle | Hamed Paknejad Ali Jalali Davoud Fadakar Ghasem Askari Hamed Azadi A DNA barcoding approach to identify species of Nemacheilidae: a complex family of cypriniform fishes in Iran Iranian Journal of Animal Biosystematics cytochrome oxidase dna barcoding nemachilidae phylogenetic tree species identification |
| title | A DNA barcoding approach to identify species of Nemacheilidae: a complex family of cypriniform fishes in Iran |
| title_full | A DNA barcoding approach to identify species of Nemacheilidae: a complex family of cypriniform fishes in Iran |
| title_fullStr | A DNA barcoding approach to identify species of Nemacheilidae: a complex family of cypriniform fishes in Iran |
| title_full_unstemmed | A DNA barcoding approach to identify species of Nemacheilidae: a complex family of cypriniform fishes in Iran |
| title_short | A DNA barcoding approach to identify species of Nemacheilidae: a complex family of cypriniform fishes in Iran |
| title_sort | dna barcoding approach to identify species of nemacheilidae a complex family of cypriniform fishes in iran |
| topic | cytochrome oxidase dna barcoding nemachilidae phylogenetic tree species identification |
| url | https://ijab.um.ac.ir/article_30186_a8f3153e189718abee59115923c1512a.pdf |
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