Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: a new insight into an old approach
The OmlA protein is a virulence factor of Actinobacillus pleuropneumoniae, an important pathogen in pigs. The polymorphisms present in the omlA gene sequence of 15 reference serotypes of A. pleuropneumoniae and non-serotypable isolates were assessed to determine the possible evolutionary relationshi...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2013-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200015 |
| _version_ | 1819114580833468416 |
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| author | Ciro César Rossi Elza Fernandes de Araújo Marisa Vieira de Queiroz Denise Mara Soares Bazzolli |
| author_facet | Ciro César Rossi Elza Fernandes de Araújo Marisa Vieira de Queiroz Denise Mara Soares Bazzolli |
| author_sort | Ciro César Rossi |
| collection | DOAJ |
| description | The OmlA protein is a virulence factor of Actinobacillus pleuropneumoniae, an important pathogen in pigs. The polymorphisms present in the omlA gene sequence of 15 reference serotypes of A. pleuropneumoniae and non-serotypable isolates were assessed to determine the possible evolutionary relationship among them and to validate the importance of this gene as a molecular marker for the characterization of this bacterium. Divergence among the 15 serotypes of A. pleuropneumoniae probably resulted initially from two major evolutionary events that led to subsequent differentiation into nine groups. This differentiation makes it possible to characterize most of the serotypes by using bionformatics, thereby avoiding problems with immunological cross-reactivity. A conserved α-helix common to all the serotypes was most likely involved in connecting the protein to the outer membrane and acting as a signal peptide. A previously unknown gene duplication was also identified and could contribute to the genetic variability that makes it difficult to serotype some isolates. Our data support the importance of the omlA gene in the biology of A. pleuropneumoniae and provide a new area of research into the OmlA protein. |
| first_indexed | 2024-12-22T04:47:34Z |
| format | Article |
| id | doaj.art-041b08b3d7094cdc9ec6356cfe173462 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-22T04:47:34Z |
| publishDate | 2013-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-041b08b3d7094cdc9ec6356cfe1734622022-12-21T18:38:33ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852013-01-01362243251Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: a new insight into an old approachCiro César RossiElza Fernandes de AraújoMarisa Vieira de QueirozDenise Mara Soares BazzolliThe OmlA protein is a virulence factor of Actinobacillus pleuropneumoniae, an important pathogen in pigs. The polymorphisms present in the omlA gene sequence of 15 reference serotypes of A. pleuropneumoniae and non-serotypable isolates were assessed to determine the possible evolutionary relationship among them and to validate the importance of this gene as a molecular marker for the characterization of this bacterium. Divergence among the 15 serotypes of A. pleuropneumoniae probably resulted initially from two major evolutionary events that led to subsequent differentiation into nine groups. This differentiation makes it possible to characterize most of the serotypes by using bionformatics, thereby avoiding problems with immunological cross-reactivity. A conserved α-helix common to all the serotypes was most likely involved in connecting the protein to the outer membrane and acting as a signal peptide. A previously unknown gene duplication was also identified and could contribute to the genetic variability that makes it difficult to serotype some isolates. Our data support the importance of the omlA gene in the biology of A. pleuropneumoniae and provide a new area of research into the OmlA protein.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200015Actinobacillus pleuropneumoniaeomlA genephylogenetic reconstructionporcine pleuropneumonia |
| spellingShingle | Ciro César Rossi Elza Fernandes de Araújo Marisa Vieira de Queiroz Denise Mara Soares Bazzolli Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: a new insight into an old approach Genetics and Molecular Biology Actinobacillus pleuropneumoniae omlA gene phylogenetic reconstruction porcine pleuropneumonia |
| title | Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: a new insight into an old approach |
| title_full | Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: a new insight into an old approach |
| title_fullStr | Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: a new insight into an old approach |
| title_full_unstemmed | Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: a new insight into an old approach |
| title_short | Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: a new insight into an old approach |
| title_sort | characterization of the omla gene from different serotypes of actinobacillus pleuropneumoniae a new insight into an old approach |
| topic | Actinobacillus pleuropneumoniae omlA gene phylogenetic reconstruction porcine pleuropneumonia |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200015 |
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