Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats
Traumatic spinal cord injury results in severe neurological deficits, mostly irreversible. The cell therapy represents a strategy for treatment particularly with the use of stem cells with satisfactory results in several experimental models. The aim of the study was to compare the treatment of spina...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2016-06-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21596 |
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| author | Marta Rocha Araujo Pablo Herthel Carvalho Taís Silva de Paula Bárbara Silva Okano Ricardo Junqueira Del Carlo Rômulo Dias Novaes Daise Nunes Queiroz da Cunha Clóvis Andrade Neves |
| author_facet | Marta Rocha Araujo Pablo Herthel Carvalho Taís Silva de Paula Bárbara Silva Okano Ricardo Junqueira Del Carlo Rômulo Dias Novaes Daise Nunes Queiroz da Cunha Clóvis Andrade Neves |
| author_sort | Marta Rocha Araujo |
| collection | DOAJ |
| description | Traumatic spinal cord injury results in severe neurological deficits, mostly irreversible. The cell therapy represents a strategy for treatment particularly with the use of stem cells with satisfactory results in several experimental models. The aim of the study was to compare the treatment of spinal cord injury (SCI) with and without mesenchymal stem cells (MSC), to investigate whether MSCs migrate and/or remain at the site of injury, and to analyze the effects of MSCs on inflammation, astrocytic reactivity and activation of endogenous stem cells. Three hours after SCI, animals received bone marrow-derived MSCs (1×107 in 1mL PBS, IV). Animals were euthanized 24 hours, 7 and 21 days post-injury. The MSC were not present in the site of the lesion and the immunofluorescent evaluation showed significant attenuation of inflammatory response with reduction in macrophages labeled with anti-CD68 antibody (ED1), decreased immunoreactivity of astrocytes (GFAP+) and greater activation of endogenous stem cells (nestin+) in the treated groups. Therefore, cell transplantation have a positive effect on recovery from traumatic spinal cord injury possibly due to the potential of MSCs to attenuate the immune response. |
| first_indexed | 2024-12-13T09:45:54Z |
| format | Article |
| id | doaj.art-041b78f4ac7e416290fd437dfe0cda3d |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-12-13T09:45:54Z |
| publishDate | 2016-06-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-041b78f4ac7e416290fd437dfe0cda3d2022-12-21T23:52:04ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592016-06-013731355136810.5433/1679-0359.2016v37n3p135513946Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of ratsMarta Rocha Araujo0Pablo Herthel Carvalho1Taís Silva de Paula2Bárbara Silva Okano3Ricardo Junqueira Del Carlo4Rômulo Dias Novaes5Daise Nunes Queiroz da Cunha6Clóvis Andrade Neves7Universidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de AlfenasUniversidade Federal de ViçosaUniversidade Federal de AlfenasTraumatic spinal cord injury results in severe neurological deficits, mostly irreversible. The cell therapy represents a strategy for treatment particularly with the use of stem cells with satisfactory results in several experimental models. The aim of the study was to compare the treatment of spinal cord injury (SCI) with and without mesenchymal stem cells (MSC), to investigate whether MSCs migrate and/or remain at the site of injury, and to analyze the effects of MSCs on inflammation, astrocytic reactivity and activation of endogenous stem cells. Three hours after SCI, animals received bone marrow-derived MSCs (1×107 in 1mL PBS, IV). Animals were euthanized 24 hours, 7 and 21 days post-injury. The MSC were not present in the site of the lesion and the immunofluorescent evaluation showed significant attenuation of inflammatory response with reduction in macrophages labeled with anti-CD68 antibody (ED1), decreased immunoreactivity of astrocytes (GFAP+) and greater activation of endogenous stem cells (nestin+) in the treated groups. Therefore, cell transplantation have a positive effect on recovery from traumatic spinal cord injury possibly due to the potential of MSCs to attenuate the immune response.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21596Cellular therapyNeurosurgeryStem cell. |
| spellingShingle | Marta Rocha Araujo Pablo Herthel Carvalho Taís Silva de Paula Bárbara Silva Okano Ricardo Junqueira Del Carlo Rômulo Dias Novaes Daise Nunes Queiroz da Cunha Clóvis Andrade Neves Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats Semina: Ciências Agrárias Cellular therapy Neurosurgery Stem cell. |
| title | Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats |
| title_full | Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats |
| title_fullStr | Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats |
| title_full_unstemmed | Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats |
| title_short | Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats |
| title_sort | mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats |
| topic | Cellular therapy Neurosurgery Stem cell. |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21596 |
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