Prognostic significance of miR-205 in endometrial cancer.
microRNAs have emerged as key regulators of gene expression, and their altered expression has been associated with tumorigenesis and tumor progression. Thus, microRNAs have potential as both cancer biomarkers and/or potential novel therapeutic targets. Although accumulating evidence suggests the rol...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3325973?pdf=render |
| _version_ | 1811281632912474112 |
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| author | Mihriban Karaayvaz Cecilia Zhang Sharon Liang Kenneth R Shroyer Jingfang Ju |
| author_facet | Mihriban Karaayvaz Cecilia Zhang Sharon Liang Kenneth R Shroyer Jingfang Ju |
| author_sort | Mihriban Karaayvaz |
| collection | DOAJ |
| description | microRNAs have emerged as key regulators of gene expression, and their altered expression has been associated with tumorigenesis and tumor progression. Thus, microRNAs have potential as both cancer biomarkers and/or potential novel therapeutic targets. Although accumulating evidence suggests the role of aberrant microRNA expression in endometrial carcinogenesis, there are still limited data available about the prognostic significance of microRNAs in endometrial cancer. The goal of this study is to investigate the prognostic value of selected key microRNAs in endometrial cancer by the analysis of archival formalin-fixed paraffin-embedded tissues.Total RNAs were extracted from 48 paired normal and endometrial tumor specimens using Trizol based approach. The expression of miR-26a, let-7g, miR-21, miR-181b, miR-200c, miR-192, miR-215, miR-200c, and miR-205 were quantified by real time qRT-PCR expression analysis. Targets of the differentially expressed miRNAs were quantified using immunohistochemistry. Statistical analysis was performed by GraphPad Prism 5.0.The expression levels of miR-200c (P<0.0001) and miR-205 (P<0.0001) were significantly increased in endometrial tumors compared to normal tissues. Kaplan-Meier survival analysis revealed that high levels of miR-205 expression were associated with poor patient overall survival (hazard ratio, 0.377; Logrank test, P = 0.028). Furthermore, decreased expression of a miR-205 target PTEN was detected in endometrial cancer tissues compared to normal tissues.miR-205 holds a unique potential as a prognostic biomarker in endometrial cancer. |
| first_indexed | 2024-04-13T01:36:00Z |
| format | Article |
| id | doaj.art-041c94dab13345bf82f1184db8ece458 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T01:36:00Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-041c94dab13345bf82f1184db8ece4582022-12-22T03:08:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3515810.1371/journal.pone.0035158Prognostic significance of miR-205 in endometrial cancer.Mihriban KaraayvazCecilia ZhangSharon LiangKenneth R ShroyerJingfang JumicroRNAs have emerged as key regulators of gene expression, and their altered expression has been associated with tumorigenesis and tumor progression. Thus, microRNAs have potential as both cancer biomarkers and/or potential novel therapeutic targets. Although accumulating evidence suggests the role of aberrant microRNA expression in endometrial carcinogenesis, there are still limited data available about the prognostic significance of microRNAs in endometrial cancer. The goal of this study is to investigate the prognostic value of selected key microRNAs in endometrial cancer by the analysis of archival formalin-fixed paraffin-embedded tissues.Total RNAs were extracted from 48 paired normal and endometrial tumor specimens using Trizol based approach. The expression of miR-26a, let-7g, miR-21, miR-181b, miR-200c, miR-192, miR-215, miR-200c, and miR-205 were quantified by real time qRT-PCR expression analysis. Targets of the differentially expressed miRNAs were quantified using immunohistochemistry. Statistical analysis was performed by GraphPad Prism 5.0.The expression levels of miR-200c (P<0.0001) and miR-205 (P<0.0001) were significantly increased in endometrial tumors compared to normal tissues. Kaplan-Meier survival analysis revealed that high levels of miR-205 expression were associated with poor patient overall survival (hazard ratio, 0.377; Logrank test, P = 0.028). Furthermore, decreased expression of a miR-205 target PTEN was detected in endometrial cancer tissues compared to normal tissues.miR-205 holds a unique potential as a prognostic biomarker in endometrial cancer.http://europepmc.org/articles/PMC3325973?pdf=render |
| spellingShingle | Mihriban Karaayvaz Cecilia Zhang Sharon Liang Kenneth R Shroyer Jingfang Ju Prognostic significance of miR-205 in endometrial cancer. PLoS ONE |
| title | Prognostic significance of miR-205 in endometrial cancer. |
| title_full | Prognostic significance of miR-205 in endometrial cancer. |
| title_fullStr | Prognostic significance of miR-205 in endometrial cancer. |
| title_full_unstemmed | Prognostic significance of miR-205 in endometrial cancer. |
| title_short | Prognostic significance of miR-205 in endometrial cancer. |
| title_sort | prognostic significance of mir 205 in endometrial cancer |
| url | http://europepmc.org/articles/PMC3325973?pdf=render |
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