Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching
Sulfobacillus and Leptospirillum occur frequently in leaching systems. Here we investigated the effects of cells of L. ferriphilum on biofilm formation and leaching performance by S. thermosulfidooxidans. The effects were caused by the presence of L. ferriphilum or an addition of pyrite leach liquor...
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00044/full |
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| author | Qian Li Jianyu Zhu Shoupeng Li Ruiyong Zhang Tangfu Xiao Wolfgang Sand Wolfgang Sand |
| author_facet | Qian Li Jianyu Zhu Shoupeng Li Ruiyong Zhang Tangfu Xiao Wolfgang Sand Wolfgang Sand |
| author_sort | Qian Li |
| collection | DOAJ |
| description | Sulfobacillus and Leptospirillum occur frequently in leaching systems. Here we investigated the effects of cells of L. ferriphilum on biofilm formation and leaching performance by S. thermosulfidooxidans. The effects were caused by the presence of L. ferriphilum or an addition of pyrite leach liquor from L. ferriphilum. Data show that the number of attached S. thermosulfidooxidans on pyrite increases, if the pyrite had been pre-colonized by living biofilms of L. ferriphilum, while it decreases if the pre-colonized biofilms had been inactivated. Coaggregation between S. thermosulfidooxidans and L. ferriphilum occurs during the dual-species biofilm formation, but different effects on bioleaching were noted, if the preculture of L. ferriphilum had been different. If L. ferriphilum had been pre-colonized on a pyrite, significantly negative effect was shown. However, if the two species were simultaneously inoculated into a sterile leaching system, the bioleaching efficiency was better than that of a pure culture of S. thermosulfidooxidans. The effect might be related to a metabolic preference of S. thermosulfidooxidans. If S. thermosulfidooxidans performed leaching in a filtered pyrite leachate from L. ferriphilum, the cells preferred to oxidize RISCs instead of ferrous ion and the number of attached cells decreased compared with the control. This study gives an indication that in a short-term multi-species leaching system the role of S. thermosufidooxidans may be related to the time of its introduction. |
| first_indexed | 2024-12-10T13:00:11Z |
| format | Article |
| id | doaj.art-041e18c707b2466da1a9fa30374218a0 |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T13:00:11Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-041e18c707b2466da1a9fa30374218a02022-12-22T01:47:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-01-011110.3389/fmicb.2020.00044500335Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite BioleachingQian Li0Jianyu Zhu1Shoupeng Li2Ruiyong Zhang3Tangfu Xiao4Wolfgang Sand5Wolfgang Sand6Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou, ChinaKey Laboratory of Biometallurgy of Ministry of Education, School of Minerals Processing and Bioengineering, Central South University, Changsha, ChinaSchool of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou, ChinaFederal Institute for Geosciences and Natural Resources (BGR), Hanover, GermanyKey Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou, ChinaCollege of Environmental Science and Engineering, Donghua University, Shanghai, ChinaBiofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen, Essen, GermanySulfobacillus and Leptospirillum occur frequently in leaching systems. Here we investigated the effects of cells of L. ferriphilum on biofilm formation and leaching performance by S. thermosulfidooxidans. The effects were caused by the presence of L. ferriphilum or an addition of pyrite leach liquor from L. ferriphilum. Data show that the number of attached S. thermosulfidooxidans on pyrite increases, if the pyrite had been pre-colonized by living biofilms of L. ferriphilum, while it decreases if the pre-colonized biofilms had been inactivated. Coaggregation between S. thermosulfidooxidans and L. ferriphilum occurs during the dual-species biofilm formation, but different effects on bioleaching were noted, if the preculture of L. ferriphilum had been different. If L. ferriphilum had been pre-colonized on a pyrite, significantly negative effect was shown. However, if the two species were simultaneously inoculated into a sterile leaching system, the bioleaching efficiency was better than that of a pure culture of S. thermosulfidooxidans. The effect might be related to a metabolic preference of S. thermosulfidooxidans. If S. thermosulfidooxidans performed leaching in a filtered pyrite leachate from L. ferriphilum, the cells preferred to oxidize RISCs instead of ferrous ion and the number of attached cells decreased compared with the control. This study gives an indication that in a short-term multi-species leaching system the role of S. thermosufidooxidans may be related to the time of its introduction.https://www.frontiersin.org/article/10.3389/fmicb.2020.00044/fullSulfobacillus thermosulfidooxidansLeptospirillum ferriphilumbioleachingadhesionbiofilm |
| spellingShingle | Qian Li Jianyu Zhu Shoupeng Li Ruiyong Zhang Tangfu Xiao Wolfgang Sand Wolfgang Sand Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching Frontiers in Microbiology Sulfobacillus thermosulfidooxidans Leptospirillum ferriphilum bioleaching adhesion biofilm |
| title | Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching |
| title_full | Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching |
| title_fullStr | Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching |
| title_full_unstemmed | Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching |
| title_short | Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching |
| title_sort | interactions between cells of sulfobacillus thermosulfidooxidans and leptospirillum ferriphilum during pyrite bioleaching |
| topic | Sulfobacillus thermosulfidooxidans Leptospirillum ferriphilum bioleaching adhesion biofilm |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00044/full |
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