Enhancing neuroprotective effect of aminosalicylic acid-grafted chitosan electrospun fibers for spinal cord injury
The hyperinflammation microenvironment after spinal cord injury (SCI) remains a great challenge for neural regeneration. Methylprednisolone has been used to reduce the inflammatory response after SCI, but it is controversial due to side effects associated with off-specific targeting effects. In this...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422003271 |
| _version_ | 1797938017406550016 |
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| author | Dahao Wang Haosen Zhao Chang Xu Sen Lin Yue Guo |
| author_facet | Dahao Wang Haosen Zhao Chang Xu Sen Lin Yue Guo |
| author_sort | Dahao Wang |
| collection | DOAJ |
| description | The hyperinflammation microenvironment after spinal cord injury (SCI) remains a great challenge for neural regeneration. Methylprednisolone has been used to reduce the inflammatory response after SCI, but it is controversial due to side effects associated with off-specific targeting effects. In this study, we synthesized in situ 5-ASA grafted chitosan electrospun fibers (ASA-EF) with excellent injectable and self-healing properties to reprogram nerve cells via displaying biological distribution, gene expression, and functional changes. With the support of ASA-EF, the downregulation of inflammatory cytokines expression and the upregulation of anti-inflammatory and regenerative gene expression were found in vitro studies. Moreover, ASA-EF administration polarized macrophages toward proregenerative phenotypes in the injured lesion, and significantly reduced cavity area. In addition, ASA-EF administration increased myelination and regenerating axons and improved motor function (score of 5 versus 2 for SCI group). These results illustrate that the neuroprotective effect of this artificial nanoplatform will facilitate the clinical treatment of traumatic-related diseases via forming a recycled microenvironment that supports regeneration and functional recovery. These particles may be applied to trauma and potential other inflammatory diseases. |
| first_indexed | 2024-04-10T18:54:15Z |
| format | Article |
| id | doaj.art-041f3ac5d9534f24adcb1d3b9c133c76 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-10T18:54:15Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-041f3ac5d9534f24adcb1d3b9c133c762023-02-01T04:27:23ZengElsevierMaterials Today Bio2590-00642023-02-0118100529Enhancing neuroprotective effect of aminosalicylic acid-grafted chitosan electrospun fibers for spinal cord injuryDahao Wang0Haosen Zhao1Chang Xu2Sen Lin3Yue Guo4Department of Orthopedic, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China; Key Laboratory of Medical Tissue Engineering, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, PR ChinaKey Laboratory of Medical Tissue Engineering, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, PR ChinaDepartment of Orthopedic, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, ChinaKey Laboratory of Medical Tissue Engineering, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, PR China; Corresponding author.Department of Orthopedic, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China; Key Laboratory of Medical Tissue Engineering, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, PR China; Corresponding author. Department of Orthopedic, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.The hyperinflammation microenvironment after spinal cord injury (SCI) remains a great challenge for neural regeneration. Methylprednisolone has been used to reduce the inflammatory response after SCI, but it is controversial due to side effects associated with off-specific targeting effects. In this study, we synthesized in situ 5-ASA grafted chitosan electrospun fibers (ASA-EF) with excellent injectable and self-healing properties to reprogram nerve cells via displaying biological distribution, gene expression, and functional changes. With the support of ASA-EF, the downregulation of inflammatory cytokines expression and the upregulation of anti-inflammatory and regenerative gene expression were found in vitro studies. Moreover, ASA-EF administration polarized macrophages toward proregenerative phenotypes in the injured lesion, and significantly reduced cavity area. In addition, ASA-EF administration increased myelination and regenerating axons and improved motor function (score of 5 versus 2 for SCI group). These results illustrate that the neuroprotective effect of this artificial nanoplatform will facilitate the clinical treatment of traumatic-related diseases via forming a recycled microenvironment that supports regeneration and functional recovery. These particles may be applied to trauma and potential other inflammatory diseases.http://www.sciencedirect.com/science/article/pii/S2590006422003271Electrospun fibersSpinal cord injuryASANerve regenerationImmunoengineering |
| spellingShingle | Dahao Wang Haosen Zhao Chang Xu Sen Lin Yue Guo Enhancing neuroprotective effect of aminosalicylic acid-grafted chitosan electrospun fibers for spinal cord injury Materials Today Bio Electrospun fibers Spinal cord injury ASA Nerve regeneration Immunoengineering |
| title | Enhancing neuroprotective effect of aminosalicylic acid-grafted chitosan electrospun fibers for spinal cord injury |
| title_full | Enhancing neuroprotective effect of aminosalicylic acid-grafted chitosan electrospun fibers for spinal cord injury |
| title_fullStr | Enhancing neuroprotective effect of aminosalicylic acid-grafted chitosan electrospun fibers for spinal cord injury |
| title_full_unstemmed | Enhancing neuroprotective effect of aminosalicylic acid-grafted chitosan electrospun fibers for spinal cord injury |
| title_short | Enhancing neuroprotective effect of aminosalicylic acid-grafted chitosan electrospun fibers for spinal cord injury |
| title_sort | enhancing neuroprotective effect of aminosalicylic acid grafted chitosan electrospun fibers for spinal cord injury |
| topic | Electrospun fibers Spinal cord injury ASA Nerve regeneration Immunoengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422003271 |
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