The effect of L-glutamic acid and N-acetylcysteine administration on biochemical blood parameters in rats treated with CCl(4)
A toxic organic substance CCl4 is a well known model compound for studying detoxification function of the liver and developing oxidative stress. The goal of the study was to estimate the effect of L-glutamic acid (L-Glu) and N-acetylcysteine (NAC) administration on rat blood parameters upon the toxi...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2023-04-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2023/06/Salyha_95_2.pdf |
| _version_ | 1797654469294424064 |
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| author | N. O. Salyha |
| author_facet | N. O. Salyha |
| author_sort | N. O. Salyha |
| collection | DOAJ |
| description | A toxic organic substance CCl4 is a well known model compound for studying detoxification function of the liver and developing oxidative stress. The goal of the study was to estimate the effect of L-glutamic acid (L-Glu) and N-acetylcysteine (NAC) administration on rat blood parameters upon the toxic effects of CCl4. Experimental male Wistar rats were injected intraperitoneally with CCl4, the rats of CCl4/L‑Glu group were additionally injected with L-Glu (750 mg/kg), of CCl4/NAC group – with NAC (150 mg/kg), of CCl4/L-Glu/NAC group – with L-Glu (750 mg/kg) and NAC (150 mg/kg). The duration of the experiment was 24 h. Increased level of lipid peroxides, TBARS, triacylglycerols, cholesterol and decreased glutathione peroxidase, glutathione reductase, glutathione-S-transferase activity and GSH content were observed in the blood of the CCl4 treated animals compared to the control untreated group. When animals were additionally injected with L-Glu or L-Glu/NAC, the most of the studied indicators were shown to be close to the control level These results suggest that the mentioned above aminoacids attenuated CCl4-induced oxidative stress in the blood of rats. |
| first_indexed | 2024-03-11T16:59:56Z |
| format | Article |
| id | doaj.art-0427874918e24924b9b0cc8a36eb0401 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T16:59:56Z |
| publishDate | 2023-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-0427874918e24924b9b0cc8a36eb04012023-10-20T10:00:44ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032023-04-01952687410.15407/ubj95.02.068The effect of L-glutamic acid and N-acetylcysteine administration on biochemical blood parameters in rats treated with CCl(4)N. O. Salyha0https://orcid.org/0000-0003-0592-407XInstitute of Animal Biology NAAS of Ukraine, Lviv, UkraineA toxic organic substance CCl4 is a well known model compound for studying detoxification function of the liver and developing oxidative stress. The goal of the study was to estimate the effect of L-glutamic acid (L-Glu) and N-acetylcysteine (NAC) administration on rat blood parameters upon the toxic effects of CCl4. Experimental male Wistar rats were injected intraperitoneally with CCl4, the rats of CCl4/L‑Glu group were additionally injected with L-Glu (750 mg/kg), of CCl4/NAC group – with NAC (150 mg/kg), of CCl4/L-Glu/NAC group – with L-Glu (750 mg/kg) and NAC (150 mg/kg). The duration of the experiment was 24 h. Increased level of lipid peroxides, TBARS, triacylglycerols, cholesterol and decreased glutathione peroxidase, glutathione reductase, glutathione-S-transferase activity and GSH content were observed in the blood of the CCl4 treated animals compared to the control untreated group. When animals were additionally injected with L-Glu or L-Glu/NAC, the most of the studied indicators were shown to be close to the control level These results suggest that the mentioned above aminoacids attenuated CCl4-induced oxidative stress in the blood of rats.http://ukrbiochemjournal.org/wp-content/uploads/2023/06/Salyha_95_2.pdfbiochemical blood parametersglutathione dependent enzymesl-glutamic acidn-acetylcysteineoxidative stresstetrachloromethane |
| spellingShingle | N. O. Salyha The effect of L-glutamic acid and N-acetylcysteine administration on biochemical blood parameters in rats treated with CCl(4) The Ukrainian Biochemical Journal biochemical blood parameters glutathione dependent enzymes l-glutamic acid n-acetylcysteine oxidative stress tetrachloromethane |
| title | The effect of L-glutamic acid and N-acetylcysteine administration on biochemical blood parameters in rats treated with CCl(4) |
| title_full | The effect of L-glutamic acid and N-acetylcysteine administration on biochemical blood parameters in rats treated with CCl(4) |
| title_fullStr | The effect of L-glutamic acid and N-acetylcysteine administration on biochemical blood parameters in rats treated with CCl(4) |
| title_full_unstemmed | The effect of L-glutamic acid and N-acetylcysteine administration on biochemical blood parameters in rats treated with CCl(4) |
| title_short | The effect of L-glutamic acid and N-acetylcysteine administration on biochemical blood parameters in rats treated with CCl(4) |
| title_sort | effect of l glutamic acid and n acetylcysteine administration on biochemical blood parameters in rats treated with ccl 4 |
| topic | biochemical blood parameters glutathione dependent enzymes l-glutamic acid n-acetylcysteine oxidative stress tetrachloromethane |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2023/06/Salyha_95_2.pdf |
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