Specific Host-Responsive Associations Between Medicago truncatula Accessions and Sinorhizobium Strains
Legume plants interact with rhizobia to form nitrogen-fixing root nodules. Legume-rhizobium interactions are specific and only compatible rhizobia and plant species will lead to nodule formation. Even within compatible interactions, the genotype of both the plant and the bacterial symbiont will impa...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2017-05-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-17-0009-R |
| _version_ | 1819081648281485312 |
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| author | Théophile Kazmierczak Marianna Nagymihály Florian Lamouche Quentin Barrière Ibtissem Guefrachi Benoit Alunni Mouna Ouadghiri Jamal Ibijbijen Éva Kondorosi Peter Mergaert Véronique Gruber |
| author_facet | Théophile Kazmierczak Marianna Nagymihály Florian Lamouche Quentin Barrière Ibtissem Guefrachi Benoit Alunni Mouna Ouadghiri Jamal Ibijbijen Éva Kondorosi Peter Mergaert Véronique Gruber |
| author_sort | Théophile Kazmierczak |
| collection | DOAJ |
| description | Legume plants interact with rhizobia to form nitrogen-fixing root nodules. Legume-rhizobium interactions are specific and only compatible rhizobia and plant species will lead to nodule formation. Even within compatible interactions, the genotype of both the plant and the bacterial symbiont will impact on the efficiency of nodule functioning and nitrogen-fixation activity. The model legume Medicago truncatula forms nodules with several species of the Sinorhizobium genus. However, the efficiency of these bacterial strains is highly variable. In this study, we compared the symbiotic efficiency of Sinorhizobium meliloti strains Sm1021, 102F34, and FSM-MA, and Sinorhizobium medicae strain WSM419 on the two widely used M. truncatula accessions A17 and R108. The efficiency of the interactions was determined by multiple parameters. We found a high effectiveness of the FSM-MA strain with both M. truncatula accessions. In contrast, specific highly efficient interactions were obtained for the A17-WSM419 and R108-102F34 combinations. Remarkably, the widely used Sm1021 strain performed weakly on both hosts. We showed that Sm1021 efficiently induced nodule organogenesis but cannot fully activate the differentiation of the symbiotic nodule cells, explaining its weaker performance. These results will be informative for the selection of appropriate rhizobium strains in functional studies on symbiosis using these M. truncatula accessions, particularly for research focusing on late stages of the nodulation process. |
| first_indexed | 2024-12-21T20:04:07Z |
| format | Article |
| id | doaj.art-042a0583a3ae47989b7a37e0a47377da |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-21T20:04:07Z |
| publishDate | 2017-05-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-042a0583a3ae47989b7a37e0a47377da2022-12-21T18:51:53ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062017-05-0130539940910.1094/MPMI-01-17-0009-RSpecific Host-Responsive Associations Between Medicago truncatula Accessions and Sinorhizobium StrainsThéophile Kazmierczak0Marianna Nagymihály1Florian Lamouche2Quentin Barrière3Ibtissem Guefrachi4Benoit Alunni5Mouna Ouadghiri6Jamal Ibijbijen7Éva Kondorosi8Peter Mergaert9Véronique Gruber10Institute of Plant Sciences Paris Saclay IPS2, CNRS, INRA, Université Paris-Sud, Université Evry, Université Paris-Saclay, Bâtiment 630, 91405 Orsay, France. Institute of Plant Sciences Paris-Saclay IPS2, Université Paris Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405, Orsay, France;Institute for Integrative Biology of the Cell, UMR 9198, CNRS/Université Paris-Sud/CEA, 91198, Gif-sur-Yvette, France;Institute for Integrative Biology of the Cell, UMR 9198, CNRS/Université Paris-Sud/CEA, 91198, Gif-sur-Yvette, France;Institute for Integrative Biology of the Cell, UMR 9198, CNRS/Université Paris-Sud/CEA, 91198, Gif-sur-Yvette, France;Institute for Integrative Biology of the Cell, UMR 9198, CNRS/Université Paris-Sud/CEA, 91198, Gif-sur-Yvette, France;Institute for Integrative Biology of the Cell, UMR 9198, CNRS/Université Paris-Sud/CEA, 91198, Gif-sur-Yvette, France;Collections Coordonnées Marocaines de Microorganismes et Laboratoire de Microbiologie et Biologie Moléculaire, Centre National pour la Recherche Scientifique et Technique, Rabat, Maroc; andFaculté des Sciences, Université Moulay Ismail, Meknès, Maroc, Faculté des Sciences, Université Moulay Ismail, BP 11201 Zitoune, Meknès, MarocInstitute for Integrative Biology of the Cell, UMR 9198, CNRS/Université Paris-Sud/CEA, 91198, Gif-sur-Yvette, France;Institute for Integrative Biology of the Cell, UMR 9198, CNRS/Université Paris-Sud/CEA, 91198, Gif-sur-Yvette, France;Institute of Plant Sciences Paris Saclay IPS2, CNRS, INRA, Université Paris-Sud, Université Evry, Université Paris-Saclay, Bâtiment 630, 91405 Orsay, France. Institute of Plant Sciences Paris-Saclay IPS2, Université Paris Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405, Orsay, France;Legume plants interact with rhizobia to form nitrogen-fixing root nodules. Legume-rhizobium interactions are specific and only compatible rhizobia and plant species will lead to nodule formation. Even within compatible interactions, the genotype of both the plant and the bacterial symbiont will impact on the efficiency of nodule functioning and nitrogen-fixation activity. The model legume Medicago truncatula forms nodules with several species of the Sinorhizobium genus. However, the efficiency of these bacterial strains is highly variable. In this study, we compared the symbiotic efficiency of Sinorhizobium meliloti strains Sm1021, 102F34, and FSM-MA, and Sinorhizobium medicae strain WSM419 on the two widely used M. truncatula accessions A17 and R108. The efficiency of the interactions was determined by multiple parameters. We found a high effectiveness of the FSM-MA strain with both M. truncatula accessions. In contrast, specific highly efficient interactions were obtained for the A17-WSM419 and R108-102F34 combinations. Remarkably, the widely used Sm1021 strain performed weakly on both hosts. We showed that Sm1021 efficiently induced nodule organogenesis but cannot fully activate the differentiation of the symbiotic nodule cells, explaining its weaker performance. These results will be informative for the selection of appropriate rhizobium strains in functional studies on symbiosis using these M. truncatula accessions, particularly for research focusing on late stages of the nodulation process.https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-17-0009-R |
| spellingShingle | Théophile Kazmierczak Marianna Nagymihály Florian Lamouche Quentin Barrière Ibtissem Guefrachi Benoit Alunni Mouna Ouadghiri Jamal Ibijbijen Éva Kondorosi Peter Mergaert Véronique Gruber Specific Host-Responsive Associations Between Medicago truncatula Accessions and Sinorhizobium Strains Molecular Plant-Microbe Interactions |
| title | Specific Host-Responsive Associations Between Medicago truncatula Accessions and Sinorhizobium Strains |
| title_full | Specific Host-Responsive Associations Between Medicago truncatula Accessions and Sinorhizobium Strains |
| title_fullStr | Specific Host-Responsive Associations Between Medicago truncatula Accessions and Sinorhizobium Strains |
| title_full_unstemmed | Specific Host-Responsive Associations Between Medicago truncatula Accessions and Sinorhizobium Strains |
| title_short | Specific Host-Responsive Associations Between Medicago truncatula Accessions and Sinorhizobium Strains |
| title_sort | specific host responsive associations between medicago truncatula accessions and sinorhizobium strains |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-17-0009-R |
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