Spatial heterogeneity of the shorebird gastrointestinal microbiome
The gastrointestinal tract (GIT) consists of connected structures that vary in function and physiology, and different GIT sections potentially provide different habitats for microorganisms. Birds possess unique GIT structures, including the oesophagus, proventriculus, gizzard, small intestine, caeca...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Royal Society
2020-01-01
|
| Series: | Royal Society Open Science |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.191609 |
| _version_ | 1819111927335354368 |
|---|---|
| author | Kirsten Grond Hannah Guilani Sarah M. Hird |
| author_facet | Kirsten Grond Hannah Guilani Sarah M. Hird |
| author_sort | Kirsten Grond |
| collection | DOAJ |
| description | The gastrointestinal tract (GIT) consists of connected structures that vary in function and physiology, and different GIT sections potentially provide different habitats for microorganisms. Birds possess unique GIT structures, including the oesophagus, proventriculus, gizzard, small intestine, caeca and large intestine. To understand birds as hosts of microbial ecosystems, we characterized the microbial communities in six sections of the GIT of two shorebird species, the Dunlin and Semipalmated Sandpiper, identified potential host species effects on the GIT microbiome and used microbial source tracking to determine microbial origin throughout the GIT. The upper three GIT sections had higher alpha diversity and genus richness compared to the lower sections, and microbial communities in the upper GIT showed no clustering. The proventriculus and gizzard microbiomes primarily originated from upstream sections, while the majority of the large intestine microbiome originated from the caeca. The heterogeneity of the GIT sections shown in our study urges caution in equating data from faeces or a single GIT component to the entire GIT microbiome but confirms that ecologically similar species may share many attributes in GIT microbiomes. |
| first_indexed | 2024-12-22T04:05:23Z |
| format | Article |
| id | doaj.art-042a70765052403185a4e1496cb00e7c |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-22T04:05:23Z |
| publishDate | 2020-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-042a70765052403185a4e1496cb00e7c2022-12-21T18:39:39ZengThe Royal SocietyRoyal Society Open Science2054-57032020-01-017110.1098/rsos.191609191609Spatial heterogeneity of the shorebird gastrointestinal microbiomeKirsten GrondHannah GuilaniSarah M. HirdThe gastrointestinal tract (GIT) consists of connected structures that vary in function and physiology, and different GIT sections potentially provide different habitats for microorganisms. Birds possess unique GIT structures, including the oesophagus, proventriculus, gizzard, small intestine, caeca and large intestine. To understand birds as hosts of microbial ecosystems, we characterized the microbial communities in six sections of the GIT of two shorebird species, the Dunlin and Semipalmated Sandpiper, identified potential host species effects on the GIT microbiome and used microbial source tracking to determine microbial origin throughout the GIT. The upper three GIT sections had higher alpha diversity and genus richness compared to the lower sections, and microbial communities in the upper GIT showed no clustering. The proventriculus and gizzard microbiomes primarily originated from upstream sections, while the majority of the large intestine microbiome originated from the caeca. The heterogeneity of the GIT sections shown in our study urges caution in equating data from faeces or a single GIT component to the entire GIT microbiome but confirms that ecologically similar species may share many attributes in GIT microbiomes.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.19160916s rrna genecalidrisdiversitygastrointestinal tractmicrobiota |
| spellingShingle | Kirsten Grond Hannah Guilani Sarah M. Hird Spatial heterogeneity of the shorebird gastrointestinal microbiome Royal Society Open Science 16s rrna gene calidris diversity gastrointestinal tract microbiota |
| title | Spatial heterogeneity of the shorebird gastrointestinal microbiome |
| title_full | Spatial heterogeneity of the shorebird gastrointestinal microbiome |
| title_fullStr | Spatial heterogeneity of the shorebird gastrointestinal microbiome |
| title_full_unstemmed | Spatial heterogeneity of the shorebird gastrointestinal microbiome |
| title_short | Spatial heterogeneity of the shorebird gastrointestinal microbiome |
| title_sort | spatial heterogeneity of the shorebird gastrointestinal microbiome |
| topic | 16s rrna gene calidris diversity gastrointestinal tract microbiota |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.191609 |
| work_keys_str_mv | AT kirstengrond spatialheterogeneityoftheshorebirdgastrointestinalmicrobiome AT hannahguilani spatialheterogeneityoftheshorebirdgastrointestinalmicrobiome AT sarahmhird spatialheterogeneityoftheshorebirdgastrointestinalmicrobiome |