C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae)
The aim of this study was to describe mitotic and meiotic chromosomes of Cycloneda sanguinea using C-banding, fluorescent in situ hybridization (FISH) rDNA probes, and sequential FISH/Ag-NOR staining. The chromosome number was 2n = 18 + XX for females and 2n = 18 + Xy for males. The X chromosome was...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2004-01-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200011 |
| _version_ | 1819080770941091840 |
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| author | Eliane Mariza Dortas Maffei Silvia das Graças Pompolo Eduard Petitpierre |
| author_facet | Eliane Mariza Dortas Maffei Silvia das Graças Pompolo Eduard Petitpierre |
| author_sort | Eliane Mariza Dortas Maffei |
| collection | DOAJ |
| description | The aim of this study was to describe mitotic and meiotic chromosomes of Cycloneda sanguinea using C-banding, fluorescent in situ hybridization (FISH) rDNA probes, and sequential FISH/Ag-NOR staining. The chromosome number was 2n = 18 + XX for females and 2n = 18 + Xy for males. The X chromosome was metacentric and the Y chromosome was very small. During meiosis, the karyotypic meioformula was n = 9 + Xy p, and sex chromosomes configured a parachute at metaphase I. At the beginning of pachytene, bivalents were still individualized, and sex chromosomes were associated end-to-end through the heteropycnotic region of the X chromosome. Later in pachytene, further condensation led to the formation of a pseudo-ring by the sex bivalent. All chromosomes showed pericentromeric heterochromatin. FISH and sequential FISH/Ag-NOR staining evidenced the location of the nucleolar organizer region in one pair of autosomes (at spermatogonial metaphase). During meiosis, these genes were mapped to a region outside the sex vesicle by FISH, although Xy p was deeply stained with silver at metaphase I. These results suggest that these argyrophilic substances are of a nucleolar protein nature, and seem to be synthesized by a pair of autosomes and imported during meiosis (prophase I) to the sex pair, during the association of the sex chromosomes. |
| first_indexed | 2024-12-21T19:50:10Z |
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| id | doaj.art-042aea595a1c4b1695509f45a216fd01 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-21T19:50:10Z |
| publishDate | 2004-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-042aea595a1c4b1695509f45a216fd012022-12-21T18:52:14ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852004-01-0127219119510.1590/S1415-47572004000200011C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae)Eliane Mariza Dortas MaffeiSilvia das Graças PompoloEduard PetitpierreThe aim of this study was to describe mitotic and meiotic chromosomes of Cycloneda sanguinea using C-banding, fluorescent in situ hybridization (FISH) rDNA probes, and sequential FISH/Ag-NOR staining. The chromosome number was 2n = 18 + XX for females and 2n = 18 + Xy for males. The X chromosome was metacentric and the Y chromosome was very small. During meiosis, the karyotypic meioformula was n = 9 + Xy p, and sex chromosomes configured a parachute at metaphase I. At the beginning of pachytene, bivalents were still individualized, and sex chromosomes were associated end-to-end through the heteropycnotic region of the X chromosome. Later in pachytene, further condensation led to the formation of a pseudo-ring by the sex bivalent. All chromosomes showed pericentromeric heterochromatin. FISH and sequential FISH/Ag-NOR staining evidenced the location of the nucleolar organizer region in one pair of autosomes (at spermatogonial metaphase). During meiosis, these genes were mapped to a region outside the sex vesicle by FISH, although Xy p was deeply stained with silver at metaphase I. These results suggest that these argyrophilic substances are of a nucleolar protein nature, and seem to be synthesized by a pair of autosomes and imported during meiosis (prophase I) to the sex pair, during the association of the sex chromosomes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200011in situ hybridizationrDNA sequencessequential FISH/Ag-NOR stainingC-bandingCycloneda sanguinea |
| spellingShingle | Eliane Mariza Dortas Maffei Silvia das Graças Pompolo Eduard Petitpierre C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae) Genetics and Molecular Biology in situ hybridization rDNA sequences sequential FISH/Ag-NOR staining C-banding Cycloneda sanguinea |
| title | C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae) |
| title_full | C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae) |
| title_fullStr | C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae) |
| title_full_unstemmed | C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae) |
| title_short | C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae) |
| title_sort | c banding and fluorescent in situ hybridization with rdna sequences in chromosomes of cycloneda sanguinea linnaeus coleoptera coccinellidae |
| topic | in situ hybridization rDNA sequences sequential FISH/Ag-NOR staining C-banding Cycloneda sanguinea |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200011 |
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