Accumulation Patterns of Six Pyrethrin Compounds across the Flower Developmental Stages—Comparative Analysis in Six Natural Dalmatian Pyrethrum Populations
Pyrethrin is a specialized metabolite of Dalmatian pyrethrum (<i>Tanacetum cinerariifolium</i> (Trevir.) Sch. Bip.), Asteraceae, known worldwide as an effective bioinsecticide. It consists of six active compounds: Pyrethrin I and II, cinerin I and II, and jasmolin I and II. Pyrethrin acc...
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MDPI AG
2022-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/2/252 |
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| author | Martina Grdiša Nina Jeran Filip Varga Tatjana Klepo Tonka Ninčević Zlatko Šatović |
| author_facet | Martina Grdiša Nina Jeran Filip Varga Tatjana Klepo Tonka Ninčević Zlatko Šatović |
| author_sort | Martina Grdiša |
| collection | DOAJ |
| description | Pyrethrin is a specialized metabolite of Dalmatian pyrethrum (<i>Tanacetum cinerariifolium</i> (Trevir.) Sch. Bip.), Asteraceae, known worldwide as an effective bioinsecticide. It consists of six active compounds: Pyrethrin I and II, cinerin I and II, and jasmolin I and II. Pyrethrin accumulates mainly in the flower heads and its content depends on numerous factors, such as the flower developmental stage. This study aims to investigate the accumulation patterns of six pyrethrin compounds in the flower heads of Dalmatian pyrethrum over the eight developmental stages (FS1 to FS8), and to make a comparison in six natural populations. Ultrasound assisted extraction was used to extract the pyrethrin, while qualitative and quantitative analysis was performed by High performance liquid chromatography. The accumulation patterns of different pyrethrin compounds were generally similar and also synchronous between different populations, while the pyrethrin I/pyrethrin II ratio showed irregular patterns. In all populations studied, the highest increase of all compounds was observed from FS1 to FS2. Their concentration continuously increased, reaching the highest values at FS4 stage (2–5 rows of open disc flowers), and generally decreased gradually towards FS6 or FS7 and then stagnated until the FS8 stage. Despite the very similar accumulation pattern of pyrethrin compounds in the different populations, the significant differences in their content suggest a different genetic background. Knowledge of the dynamics of pyrethrin compounds accumulation across flower development stages is valuable for determining the optimal harvest time of pyrethrum flower heads. |
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| id | doaj.art-042e21418e934c18940d544899d3bf97 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T22:50:55Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-042e21418e934c18940d544899d3bf972023-11-23T18:18:42ZengMDPI AGAgronomy2073-43952022-01-0112225210.3390/agronomy12020252Accumulation Patterns of Six Pyrethrin Compounds across the Flower Developmental Stages—Comparative Analysis in Six Natural Dalmatian Pyrethrum PopulationsMartina Grdiša0Nina Jeran1Filip Varga2Tatjana Klepo3Tonka Ninčević4Zlatko Šatović5Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Svetošimunska C. 25, 10000 Zagreb, CroatiaDepartment of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Svetošimunska C. 25, 10000 Zagreb, CroatiaDepartment of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Svetošimunska C. 25, 10000 Zagreb, CroatiaCenter of Pomology, Croatian Agency for Agriculture and Food, Zvonimirova 14 A, 21210 Solin, CroatiaDepartment for Plant Sciences, Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21000 Split, CroatiaDepartment of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Svetošimunska C. 25, 10000 Zagreb, CroatiaPyrethrin is a specialized metabolite of Dalmatian pyrethrum (<i>Tanacetum cinerariifolium</i> (Trevir.) Sch. Bip.), Asteraceae, known worldwide as an effective bioinsecticide. It consists of six active compounds: Pyrethrin I and II, cinerin I and II, and jasmolin I and II. Pyrethrin accumulates mainly in the flower heads and its content depends on numerous factors, such as the flower developmental stage. This study aims to investigate the accumulation patterns of six pyrethrin compounds in the flower heads of Dalmatian pyrethrum over the eight developmental stages (FS1 to FS8), and to make a comparison in six natural populations. Ultrasound assisted extraction was used to extract the pyrethrin, while qualitative and quantitative analysis was performed by High performance liquid chromatography. The accumulation patterns of different pyrethrin compounds were generally similar and also synchronous between different populations, while the pyrethrin I/pyrethrin II ratio showed irregular patterns. In all populations studied, the highest increase of all compounds was observed from FS1 to FS2. Their concentration continuously increased, reaching the highest values at FS4 stage (2–5 rows of open disc flowers), and generally decreased gradually towards FS6 or FS7 and then stagnated until the FS8 stage. Despite the very similar accumulation pattern of pyrethrin compounds in the different populations, the significant differences in their content suggest a different genetic background. Knowledge of the dynamics of pyrethrin compounds accumulation across flower development stages is valuable for determining the optimal harvest time of pyrethrum flower heads.https://www.mdpi.com/2073-4395/12/2/252<i>Tanacetum cinerariifolium</i>Asteraceaepyrethrinharvest time |
| spellingShingle | Martina Grdiša Nina Jeran Filip Varga Tatjana Klepo Tonka Ninčević Zlatko Šatović Accumulation Patterns of Six Pyrethrin Compounds across the Flower Developmental Stages—Comparative Analysis in Six Natural Dalmatian Pyrethrum Populations Agronomy <i>Tanacetum cinerariifolium</i> Asteraceae pyrethrin harvest time |
| title | Accumulation Patterns of Six Pyrethrin Compounds across the Flower Developmental Stages—Comparative Analysis in Six Natural Dalmatian Pyrethrum Populations |
| title_full | Accumulation Patterns of Six Pyrethrin Compounds across the Flower Developmental Stages—Comparative Analysis in Six Natural Dalmatian Pyrethrum Populations |
| title_fullStr | Accumulation Patterns of Six Pyrethrin Compounds across the Flower Developmental Stages—Comparative Analysis in Six Natural Dalmatian Pyrethrum Populations |
| title_full_unstemmed | Accumulation Patterns of Six Pyrethrin Compounds across the Flower Developmental Stages—Comparative Analysis in Six Natural Dalmatian Pyrethrum Populations |
| title_short | Accumulation Patterns of Six Pyrethrin Compounds across the Flower Developmental Stages—Comparative Analysis in Six Natural Dalmatian Pyrethrum Populations |
| title_sort | accumulation patterns of six pyrethrin compounds across the flower developmental stages comparative analysis in six natural dalmatian pyrethrum populations |
| topic | <i>Tanacetum cinerariifolium</i> Asteraceae pyrethrin harvest time |
| url | https://www.mdpi.com/2073-4395/12/2/252 |
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