New insights into the genetic variability of Fasciola hepatica (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA
This study aims to investigate the level of genetic variability of Fasciola hepatica flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear mar...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2022-09-01
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| Series: | Helminthologia |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/helm-2022-0021 |
| _version_ | 1797757868702695424 |
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| author | Chaouadi M. Scarpa F. Azzena I. Cossu P. Harhoura K. Aissi M. Tazerouti F. Garippa G. Merella P. Casu M. Sanna D. |
| author_facet | Chaouadi M. Scarpa F. Azzena I. Cossu P. Harhoura K. Aissi M. Tazerouti F. Garippa G. Merella P. Casu M. Sanna D. |
| author_sort | Chaouadi M. |
| collection | DOAJ |
| description | This study aims to investigate the level of genetic variability of Fasciola hepatica flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear markers (Internal Transcribed Spacers of nuclear ribosomal DNA - ITS) for 24 F. hepatica flukes isolated from 12 cattle in North Algeria were characterised. Only two haplotypes were obtained for the COI gene, resulting in a low level of genetic variation. The analysis of variation among the COI sequences isolated from around the world did not show high levels of genetic divergence, and the phylogenetic analysis revealed a genetic similarity among F. hepatica isolates from different areas of the world. The analysis of the ITS region showed a low level of variability, which prevented obtaining informative phylogenetic and phylogeographic results. The present study also revealed that specimens of F. hepatica are genetically similar in different hosts, indicating that the genetic structure among populations of this parasite is not influenced by the host species. The low levels of genetic variation for COI and ITS regions among fluke isolates from all continents are consistent with a common origin for the flukes’ worldwide distribution. |
| first_indexed | 2024-03-12T18:20:46Z |
| format | Article |
| id | doaj.art-04322c984cc14e6baaaf0bb4378fd903 |
| institution | Directory Open Access Journal |
| issn | 1336-9083 |
| language | English |
| last_indexed | 2024-03-12T18:20:46Z |
| publishDate | 2022-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Helminthologia |
| spelling | doaj.art-04322c984cc14e6baaaf0bb4378fd9032023-08-02T08:51:22ZengSciendoHelminthologia1336-90832022-09-0159215216410.2478/helm-2022-0021New insights into the genetic variability of Fasciola hepatica (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNAChaouadi M.0Scarpa F.1Azzena I.2Cossu P.3Harhoura K.4Aissi M.5Tazerouti F.6Garippa G.7Merella P.8Casu M.9Sanna D.10Laboratory of Biodiversity and Environment: Interactions and Genomes, Faculty of Biological Sciences, University of Sciences and TechnologyHouari Boumediene, BP 32, El Alia Bab Ezzouar, Algiers, AlgeriaDepartment of Biomedical Sciences, University of Sassari, 07100Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, 07100Sassari, ItalyDepartment of Veterinary Medicine, University of Sassari, 07100Sassari, ItalyLaboratory of Animal Health and Production, Superior National Veterinary School Rabie Bouchama, Street Issad Abbes, Oued Smar, Algiers, AlgeriaLaboratory of Animal Health and Production, Superior National Veterinary School Rabie Bouchama, Street Issad Abbes, Oued Smar, Algiers, AlgeriaLaboratory of Biodiversity and Environment: Interactions and Genomes, Faculty of Biological Sciences, University of Sciences and TechnologyHouari Boumediene, BP 32, El Alia Bab Ezzouar, Algiers, AlgeriaDepartment of Veterinary Medicine, University of Sassari, 07100Sassari, ItalyDepartment of Veterinary Medicine, University of Sassari, 07100Sassari, ItalyDepartment of Veterinary Medicine, University of Sassari, 07100Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, 07100Sassari, ItalyThis study aims to investigate the level of genetic variability of Fasciola hepatica flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear markers (Internal Transcribed Spacers of nuclear ribosomal DNA - ITS) for 24 F. hepatica flukes isolated from 12 cattle in North Algeria were characterised. Only two haplotypes were obtained for the COI gene, resulting in a low level of genetic variation. The analysis of variation among the COI sequences isolated from around the world did not show high levels of genetic divergence, and the phylogenetic analysis revealed a genetic similarity among F. hepatica isolates from different areas of the world. The analysis of the ITS region showed a low level of variability, which prevented obtaining informative phylogenetic and phylogeographic results. The present study also revealed that specimens of F. hepatica are genetically similar in different hosts, indicating that the genetic structure among populations of this parasite is not influenced by the host species. The low levels of genetic variation for COI and ITS regions among fluke isolates from all continents are consistent with a common origin for the flukes’ worldwide distribution.https://doi.org/10.2478/helm-2022-0021fasciola hepaticamolecular characterizationcoiitsphylogeography |
| spellingShingle | Chaouadi M. Scarpa F. Azzena I. Cossu P. Harhoura K. Aissi M. Tazerouti F. Garippa G. Merella P. Casu M. Sanna D. New insights into the genetic variability of Fasciola hepatica (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA Helminthologia fasciola hepatica molecular characterization coi its phylogeography |
| title | New insights into the genetic variability of Fasciola hepatica (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA |
| title_full | New insights into the genetic variability of Fasciola hepatica (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA |
| title_fullStr | New insights into the genetic variability of Fasciola hepatica (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA |
| title_full_unstemmed | New insights into the genetic variability of Fasciola hepatica (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA |
| title_short | New insights into the genetic variability of Fasciola hepatica (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA |
| title_sort | new insights into the genetic variability of fasciola hepatica trematoda in algeria and relationships with other geographic regions revealed by mitochondrial dna |
| topic | fasciola hepatica molecular characterization coi its phylogeography |
| url | https://doi.org/10.2478/helm-2022-0021 |
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