A proline racemase based PCR for identification of Trypanosoma vivax in cattle blood.
A study was conducted to develop a Trypanosoma vivax (T. vivax) specific PCR based on the T. vivax proline racemase (TvPRAC) gene. Forward and reverse primers were designed that bind at 764-783 bp and 983-1002 bp of the gene. To assess its specificity, TvPRAC PCR was conducted on DNA extracted from...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3885604?pdf=render |
| _version_ | 1818873841765580800 |
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| author | Regassa Fikru Ashenafi Hagos Stijn Rogé Armando Reyna-Bello Mary Isabel Gonzatti Bekana Merga Bruno Maria Goddeeris Philippe Büscher |
| author_facet | Regassa Fikru Ashenafi Hagos Stijn Rogé Armando Reyna-Bello Mary Isabel Gonzatti Bekana Merga Bruno Maria Goddeeris Philippe Büscher |
| author_sort | Regassa Fikru |
| collection | DOAJ |
| description | A study was conducted to develop a Trypanosoma vivax (T. vivax) specific PCR based on the T. vivax proline racemase (TvPRAC) gene. Forward and reverse primers were designed that bind at 764-783 bp and 983-1002 bp of the gene. To assess its specificity, TvPRAC PCR was conducted on DNA extracted from different haemotropic pathogens: T. vivax from Nigeria, Ethiopia and Venezuela, T. congolense Savannah type, T. brucei brucei, T. evansi, T. equiperdum, T. theileri, Theileria parva, Anaplasma marginale, Babesia bovis and Babesia bigemina and from bovine, goat, mouse, camel and human blood. The analytical sensitivity of the TvPRAC PCR was compared with that of the ITS-1 PCR and the 18S PCR-RFLP on a dilution series of T. vivax DNA in water. The diagnostic performance of the three PCRs was compared on 411 Ethiopian bovine blood specimens collected in a former study. TvPRAC PCR proved to be fully specific for T. vivax, irrespective of its geographical origin. Its analytical sensitivity was lower than that of ITS-1 PCR. On these bovine specimens, TvPRAC PCR detected 8.3% T. vivax infections while ITS-1 PCR and 18S PCR-RFLP detected respectively 22.6 and 6.1% T. vivax infections. The study demonstrates that a proline racemase based PCR could be used, preferably in combination with ITS-1 PCR, as a species-specific diagnostic test for T. vivax infections worldwide. |
| first_indexed | 2024-12-19T13:01:07Z |
| format | Article |
| id | doaj.art-04367a27ad8149fab0a39a1d1a4b7d36 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T13:01:07Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-04367a27ad8149fab0a39a1d1a4b7d362022-12-21T20:20:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8481910.1371/journal.pone.0084819A proline racemase based PCR for identification of Trypanosoma vivax in cattle blood.Regassa FikruAshenafi HagosStijn RogéArmando Reyna-BelloMary Isabel GonzattiBekana MergaBruno Maria GoddeerisPhilippe BüscherA study was conducted to develop a Trypanosoma vivax (T. vivax) specific PCR based on the T. vivax proline racemase (TvPRAC) gene. Forward and reverse primers were designed that bind at 764-783 bp and 983-1002 bp of the gene. To assess its specificity, TvPRAC PCR was conducted on DNA extracted from different haemotropic pathogens: T. vivax from Nigeria, Ethiopia and Venezuela, T. congolense Savannah type, T. brucei brucei, T. evansi, T. equiperdum, T. theileri, Theileria parva, Anaplasma marginale, Babesia bovis and Babesia bigemina and from bovine, goat, mouse, camel and human blood. The analytical sensitivity of the TvPRAC PCR was compared with that of the ITS-1 PCR and the 18S PCR-RFLP on a dilution series of T. vivax DNA in water. The diagnostic performance of the three PCRs was compared on 411 Ethiopian bovine blood specimens collected in a former study. TvPRAC PCR proved to be fully specific for T. vivax, irrespective of its geographical origin. Its analytical sensitivity was lower than that of ITS-1 PCR. On these bovine specimens, TvPRAC PCR detected 8.3% T. vivax infections while ITS-1 PCR and 18S PCR-RFLP detected respectively 22.6 and 6.1% T. vivax infections. The study demonstrates that a proline racemase based PCR could be used, preferably in combination with ITS-1 PCR, as a species-specific diagnostic test for T. vivax infections worldwide.http://europepmc.org/articles/PMC3885604?pdf=render |
| spellingShingle | Regassa Fikru Ashenafi Hagos Stijn Rogé Armando Reyna-Bello Mary Isabel Gonzatti Bekana Merga Bruno Maria Goddeeris Philippe Büscher A proline racemase based PCR for identification of Trypanosoma vivax in cattle blood. PLoS ONE |
| title | A proline racemase based PCR for identification of Trypanosoma vivax in cattle blood. |
| title_full | A proline racemase based PCR for identification of Trypanosoma vivax in cattle blood. |
| title_fullStr | A proline racemase based PCR for identification of Trypanosoma vivax in cattle blood. |
| title_full_unstemmed | A proline racemase based PCR for identification of Trypanosoma vivax in cattle blood. |
| title_short | A proline racemase based PCR for identification of Trypanosoma vivax in cattle blood. |
| title_sort | proline racemase based pcr for identification of trypanosoma vivax in cattle blood |
| url | http://europepmc.org/articles/PMC3885604?pdf=render |
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