A proline racemase based PCR for identification of Trypanosoma vivax in cattle blood.

A proline racemase based PCR for identification of Trypanosoma vivax in cattle blood.

A study was conducted to develop a Trypanosoma vivax (T. vivax) specific PCR based on the T. vivax proline racemase (TvPRAC) gene. Forward and reverse primers were designed that bind at 764-783 bp and 983-1002 bp of the gene. To assess its specificity, TvPRAC PCR was conducted on DNA extracted from...

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Bibliographic Details
Main Authors: Regassa Fikru, Ashenafi Hagos, Stijn Rogé, Armando Reyna-Bello, Mary Isabel Gonzatti, Bekana Merga, Bruno Maria Goddeeris, Philippe Büscher
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3885604?pdf=render

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