EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers
Yellow lupine is a great model for abscission-related research given that excessive flower abortion reduces its yield. It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower aborti...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/6/3001 |
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| author | Emilia Wilmowicz Agata Kućko Wojciech Pokora Małgorzata Kapusta Katarzyna Jasieniecka-Gazarkiewicz Timothy John Tranbarger Magdalena Wolska Katarzyna Panek |
| author_facet | Emilia Wilmowicz Agata Kućko Wojciech Pokora Małgorzata Kapusta Katarzyna Jasieniecka-Gazarkiewicz Timothy John Tranbarger Magdalena Wolska Katarzyna Panek |
| author_sort | Emilia Wilmowicz |
| collection | DOAJ |
| description | Yellow lupine is a great model for abscission-related research given that excessive flower abortion reduces its yield. It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells? Therefore, we used EPIP peptide to monitor specific modifications accompanied by early steps of flower abscission directly in the AZ. EPIP stimulates the downstream elements of the pathway—<i>HAESA</i> and MITOGEN-ACTIVATED PROTEIN KINASE6 and induces cellular symptoms indicating AZ activation. The EPIP treatment disrupts redox homeostasis, involving the accumulation of H<sub>2</sub>O<sub>2</sub> and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase. A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism. Notably, the formation of a signaling molecule—phosphatidic acid is induced locally in EPIP-treated AZ. Collectively, all these changes indicate the switching of several metabolic and signaling pathways directly in the AZ in response to EPIP, which inevitably leads to flower abscission. |
| first_indexed | 2024-03-10T13:12:11Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T13:12:11Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0436808a8fa546518fe7d439f709d6ad2023-11-21T10:37:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01226300110.3390/ijms22063001EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine FlowersEmilia Wilmowicz0Agata Kućko1Wojciech Pokora2Małgorzata Kapusta3Katarzyna Jasieniecka-Gazarkiewicz4Timothy John Tranbarger5Magdalena Wolska6Katarzyna Panek7Department of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, PolandDepartment of Plant Physiology, Institute of Biology, Warsaw University of Life Sciences-SGGW (WULS-SGGW), Nowoursynowska 159 Street, 02-776 Warsaw, PolandDepartment of Plant Physiology and Biotechnology, University of Gdańsk, 59 Wita Stwosza, 80-308 Gdańsk, PolandDepartment of Plant Cytology and Embryology, University of Gdańsk, 59 Wita Stwosza, 80-308 Gdańsk, PolandIntercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdańsk, Abrahama 58, 80-307 Gdańsk, PolandUMR DIADE, IRD Centre de Montpellier, Institut de Recherche pour le Développement, Université de Montpellier, 911 Avenue Agropolis BP 64501, 34394 CEDEX 5 Montpellier, FranceDepartment of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, PolandDepartment of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, PolandYellow lupine is a great model for abscission-related research given that excessive flower abortion reduces its yield. It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells? Therefore, we used EPIP peptide to monitor specific modifications accompanied by early steps of flower abscission directly in the AZ. EPIP stimulates the downstream elements of the pathway—<i>HAESA</i> and MITOGEN-ACTIVATED PROTEIN KINASE6 and induces cellular symptoms indicating AZ activation. The EPIP treatment disrupts redox homeostasis, involving the accumulation of H<sub>2</sub>O<sub>2</sub> and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase. A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism. Notably, the formation of a signaling molecule—phosphatidic acid is induced locally in EPIP-treated AZ. Collectively, all these changes indicate the switching of several metabolic and signaling pathways directly in the AZ in response to EPIP, which inevitably leads to flower abscission.https://www.mdpi.com/1422-0067/22/6/3001cell wallcropsfatty acidsflower abscission zoneoxidative stressyellow lupine |
| spellingShingle | Emilia Wilmowicz Agata Kućko Wojciech Pokora Małgorzata Kapusta Katarzyna Jasieniecka-Gazarkiewicz Timothy John Tranbarger Magdalena Wolska Katarzyna Panek EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers International Journal of Molecular Sciences cell wall crops fatty acids flower abscission zone oxidative stress yellow lupine |
| title | EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers |
| title_full | EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers |
| title_fullStr | EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers |
| title_full_unstemmed | EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers |
| title_short | EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers |
| title_sort | epip evoked modifications of redox lipid and pectin homeostasis in the abscission zone of lupine flowers |
| topic | cell wall crops fatty acids flower abscission zone oxidative stress yellow lupine |
| url | https://www.mdpi.com/1422-0067/22/6/3001 |
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