Expression Changes of Genes Involved in Autophagy of the Endoplasmic Reticulum Network in Animal Models of Alzheimer’s Disease
Background and Aim: This study aimed to investigate the association between Endoplasmic Reticulum autophagy (ER-phagy) and Alzheimer’s Disease (AD) by analyzing the expression patterns of related genes in animal models. Methods & Materials: Microarray data of AD patients’ brain tissues were extr...
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| Format: | Article |
| Language: | fas |
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Arak Medical University
2020-05-01
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| Series: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| Subjects: | |
| Online Access: | http://jams.arakmu.ac.ir/article-1-6123-en.html |
| _version_ | 1818060788981563392 |
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| author | Zakiyeh Gharib Naser Sanchooli Nima Sanadgol |
| author_facet | Zakiyeh Gharib Naser Sanchooli Nima Sanadgol |
| author_sort | Zakiyeh Gharib |
| collection | DOAJ |
| description | Background and Aim: This study aimed to investigate the association between Endoplasmic Reticulum autophagy (ER-phagy) and Alzheimer’s Disease (AD) by analyzing the expression patterns of related genes in animal models.
Methods & Materials: Microarray data of AD patients’ brain tissues were extracted from the Gene Expression Omnibus (GEO) database. These data were first analyzed in GEO2R online tool. Then, the expression of ER-phagy related genes were isolated and the protein interaction networks were plotted by STRING database for the genes with increased expression. Finally, the relationship between the genes that had significant increased expression were designed, and the expression of new identified genes in each study was examined.
Ethical Considerations: All ethical principles were considered in this article.
Results: Genes involved in ER-phagy showed a sporadic expression in different AD models. An increase in the expression of ER-phagy regulatory 1 (FAM134B) gene was observed in studies with the mutation in both Microtubule-associated Protein Tau (MAPT) and Amyloid Precursor Protein (APP) genes. Increase in the expression of NPC intracellular cholesterol transporter 1 (NPC1) gene was observed in two studies that had mutations in APP, Presenilin 1 (PSEN1) and MAPT genes. Moreover, SEC62 homolog and Cell Cycle Progression 1 (CCPG1) genes both showed decreased expression in one study. Finally, the expression of Reticulon 3 (RTN3) was not significant in any of the studies.
Conclusion: The genes involved in ER-phagy have a sporadic expression in AD models, where only two genes FAM134B and NPC1 are involved in AD. The FAM134B gene seems to interact with the Wnk1 gene, which plays a role in cell survival and proliferation, in the hippocampus and forebrain. It also interacts with the Map1lc3b gene, which has a role in phagosome deletion and protein ubiquitination, in the forebrain. It also interacts with the Map1lc3b gene, which has a role in phagosome deletion and protein ubiquitination, in the forebrain. NPC1 had interaction with the Abcg1 gene, which activates lipid homeostasis, in the subventricular zone. |
| first_indexed | 2024-12-10T13:38:00Z |
| format | Article |
| id | doaj.art-0436defb1a864952a1086332062a9313 |
| institution | Directory Open Access Journal |
| issn | 1735-5338 2008-644X |
| language | fas |
| last_indexed | 2024-12-10T13:38:00Z |
| publishDate | 2020-05-01 |
| publisher | Arak Medical University |
| record_format | Article |
| series | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| spelling | doaj.art-0436defb1a864952a1086332062a93132022-12-22T01:46:45ZfasArak Medical UniversityMajallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk1735-53382008-644X2020-05-01232184197Expression Changes of Genes Involved in Autophagy of the Endoplasmic Reticulum Network in Animal Models of Alzheimer’s DiseaseZakiyeh Gharib0Naser Sanchooli1Nima Sanadgol2 Department of Biology, Faculty of Science, University of Zabol, Zabol, Iran. Department of Biology, Faculty of Science, University of Zabol, Zabol, Iran. Department of Biology, Faculty of Science, University of Zabol, Zabol, Iran. Background and Aim: This study aimed to investigate the association between Endoplasmic Reticulum autophagy (ER-phagy) and Alzheimer’s Disease (AD) by analyzing the expression patterns of related genes in animal models. Methods & Materials: Microarray data of AD patients’ brain tissues were extracted from the Gene Expression Omnibus (GEO) database. These data were first analyzed in GEO2R online tool. Then, the expression of ER-phagy related genes were isolated and the protein interaction networks were plotted by STRING database for the genes with increased expression. Finally, the relationship between the genes that had significant increased expression were designed, and the expression of new identified genes in each study was examined. Ethical Considerations: All ethical principles were considered in this article. Results: Genes involved in ER-phagy showed a sporadic expression in different AD models. An increase in the expression of ER-phagy regulatory 1 (FAM134B) gene was observed in studies with the mutation in both Microtubule-associated Protein Tau (MAPT) and Amyloid Precursor Protein (APP) genes. Increase in the expression of NPC intracellular cholesterol transporter 1 (NPC1) gene was observed in two studies that had mutations in APP, Presenilin 1 (PSEN1) and MAPT genes. Moreover, SEC62 homolog and Cell Cycle Progression 1 (CCPG1) genes both showed decreased expression in one study. Finally, the expression of Reticulon 3 (RTN3) was not significant in any of the studies. Conclusion: The genes involved in ER-phagy have a sporadic expression in AD models, where only two genes FAM134B and NPC1 are involved in AD. The FAM134B gene seems to interact with the Wnk1 gene, which plays a role in cell survival and proliferation, in the hippocampus and forebrain. It also interacts with the Map1lc3b gene, which has a role in phagosome deletion and protein ubiquitination, in the forebrain. It also interacts with the Map1lc3b gene, which has a role in phagosome deletion and protein ubiquitination, in the forebrain. NPC1 had interaction with the Abcg1 gene, which activates lipid homeostasis, in the subventricular zone.http://jams.arakmu.ac.ir/article-1-6123-en.htmlgene expressionalzheimer’s diseaseendoplasmic reticulumautophagymicroarray |
| spellingShingle | Zakiyeh Gharib Naser Sanchooli Nima Sanadgol Expression Changes of Genes Involved in Autophagy of the Endoplasmic Reticulum Network in Animal Models of Alzheimer’s Disease Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk gene expression alzheimer’s disease endoplasmic reticulum autophagy microarray |
| title | Expression Changes of Genes Involved in Autophagy of the Endoplasmic Reticulum Network in Animal Models of Alzheimer’s Disease |
| title_full | Expression Changes of Genes Involved in Autophagy of the Endoplasmic Reticulum Network in Animal Models of Alzheimer’s Disease |
| title_fullStr | Expression Changes of Genes Involved in Autophagy of the Endoplasmic Reticulum Network in Animal Models of Alzheimer’s Disease |
| title_full_unstemmed | Expression Changes of Genes Involved in Autophagy of the Endoplasmic Reticulum Network in Animal Models of Alzheimer’s Disease |
| title_short | Expression Changes of Genes Involved in Autophagy of the Endoplasmic Reticulum Network in Animal Models of Alzheimer’s Disease |
| title_sort | expression changes of genes involved in autophagy of the endoplasmic reticulum network in animal models of alzheimer s disease |
| topic | gene expression alzheimer’s disease endoplasmic reticulum autophagy microarray |
| url | http://jams.arakmu.ac.ir/article-1-6123-en.html |
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