Metagenomic analysis reveals symbiotic relationship among bacteria in Microcystis-dominated community
Microcystis bloom, a cyanobacterial mass occurrence often found in eutrophicated water bodies, is one of the most serious threats to freshwater ecosystems worldwide. In nature, Microcystis forms aggregates or colonies that contain heterotrophic bacteria. The Microcystis-bacteria colonies were persis...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00056/full |
| _version_ | 1819059843907977216 |
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| author | Meili eXie Meili eXie Minglei eRen Minglei eRen Chen eYang Chen eYang Haisi eYi Haisi eYi Zhe eLi Tao eLi Jindong eZhao Jindong eZhao |
| author_facet | Meili eXie Meili eXie Minglei eRen Minglei eRen Chen eYang Chen eYang Haisi eYi Haisi eYi Zhe eLi Tao eLi Jindong eZhao Jindong eZhao |
| author_sort | Meili eXie |
| collection | DOAJ |
| description | Microcystis bloom, a cyanobacterial mass occurrence often found in eutrophicated water bodies, is one of the most serious threats to freshwater ecosystems worldwide. In nature, Microcystis forms aggregates or colonies that contain heterotrophic bacteria. The Microcystis-bacteria colonies were persistent even when they were maintained in lab culture for a long period. The relationship between Microcystis and the associated bacteria was investigated by a metagenomic approach in this study. We developed a visualization-guided method of binning for genome assembly after total colony DNA sequencing. We found that the method was effective in grouping sequences and it did not require reference genome sequence. Individual genomes of the colony bacteria were obtained and they provided valuable insights into microbial community structures. Analysis of metabolic pathways based on these genomes revealed that while all heterotrophic bacteria were dependent upon Microcystis for carbon and energy, Vitamin B12 biosynthesis, which is required for growth by Microcystis, was accomplished in a cooperative fashion among the bacteria. Our analysis also suggests that individual bacteria in the colony community contributed a complete pathway for degradation of benzoate, which is inhibitory to the cyanobacterial growth, and its ecological implication for Microcystis bloom is discussed. |
| first_indexed | 2024-12-21T14:17:33Z |
| format | Article |
| id | doaj.art-04379c97453949bbb634b9f1b0010ce5 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T14:17:33Z |
| publishDate | 2016-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-04379c97453949bbb634b9f1b0010ce52022-12-21T19:00:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-02-01710.3389/fmicb.2016.00056173284Metagenomic analysis reveals symbiotic relationship among bacteria in Microcystis-dominated communityMeili eXie0Meili eXie1Minglei eRen2Minglei eRen3Chen eYang4Chen eYang5Haisi eYi6Haisi eYi7Zhe eLi8Tao eLi9Jindong eZhao10Jindong eZhao11Institute of Hydrobiology, Chinese Academy of SciencesUniversity of Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesUniversity of Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesUniversity of Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesUniversity of Chinese Academy of SciencesInstitute of Botany, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesPeking UniversityMicrocystis bloom, a cyanobacterial mass occurrence often found in eutrophicated water bodies, is one of the most serious threats to freshwater ecosystems worldwide. In nature, Microcystis forms aggregates or colonies that contain heterotrophic bacteria. The Microcystis-bacteria colonies were persistent even when they were maintained in lab culture for a long period. The relationship between Microcystis and the associated bacteria was investigated by a metagenomic approach in this study. We developed a visualization-guided method of binning for genome assembly after total colony DNA sequencing. We found that the method was effective in grouping sequences and it did not require reference genome sequence. Individual genomes of the colony bacteria were obtained and they provided valuable insights into microbial community structures. Analysis of metabolic pathways based on these genomes revealed that while all heterotrophic bacteria were dependent upon Microcystis for carbon and energy, Vitamin B12 biosynthesis, which is required for growth by Microcystis, was accomplished in a cooperative fashion among the bacteria. Our analysis also suggests that individual bacteria in the colony community contributed a complete pathway for degradation of benzoate, which is inhibitory to the cyanobacterial growth, and its ecological implication for Microcystis bloom is discussed.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00056/fullMetagenomeMicrocystisSymbiosisBinningbloom |
| spellingShingle | Meili eXie Meili eXie Minglei eRen Minglei eRen Chen eYang Chen eYang Haisi eYi Haisi eYi Zhe eLi Tao eLi Jindong eZhao Jindong eZhao Metagenomic analysis reveals symbiotic relationship among bacteria in Microcystis-dominated community Frontiers in Microbiology Metagenome Microcystis Symbiosis Binning bloom |
| title | Metagenomic analysis reveals symbiotic relationship among bacteria in Microcystis-dominated community |
| title_full | Metagenomic analysis reveals symbiotic relationship among bacteria in Microcystis-dominated community |
| title_fullStr | Metagenomic analysis reveals symbiotic relationship among bacteria in Microcystis-dominated community |
| title_full_unstemmed | Metagenomic analysis reveals symbiotic relationship among bacteria in Microcystis-dominated community |
| title_short | Metagenomic analysis reveals symbiotic relationship among bacteria in Microcystis-dominated community |
| title_sort | metagenomic analysis reveals symbiotic relationship among bacteria in microcystis dominated community |
| topic | Metagenome Microcystis Symbiosis Binning bloom |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00056/full |
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