Long-term effects of maternal exposure to surgical stress at the earliest stage of pregnancy on blood pressure and behavior in offspring of OXYS rats
The use of some assisted reproductive technologies, in particular, embryo transfer, may cause various physiological and behavioral changes in the offspring. The purpose of our study was to study the effects of surgery (which is used for embryo transfer) done with pregnant dams on the weight, blood p...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2018-01-01
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| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/1271 |
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| author | I. N. Rozhkova T. N. Igonina D. S. Ragaeva O. M. Petrova E. Yu. Brusentsev V. A. Naprimerov S. Ya. Amstislavsky |
| author_facet | I. N. Rozhkova T. N. Igonina D. S. Ragaeva O. M. Petrova E. Yu. Brusentsev V. A. Naprimerov S. Ya. Amstislavsky |
| author_sort | I. N. Rozhkova |
| collection | DOAJ |
| description | The use of some assisted reproductive technologies, in particular, embryo transfer, may cause various physiological and behavioral changes in the offspring. The purpose of our study was to study the effects of surgery (which is used for embryo transfer) done with pregnant dams on the weight, blood pressure and behavior in the open field and elevated plusmaze tests in adult offspring. Thus, longterm effects on the offspring after maternal exposure to surgical stress given to dams at the 4th day of pregnancy were studied in OXYS rats. OXYS females were mated in estrus with fertile males of the same strain. 96 hours after spermatozoa were found in vaginal smears the surgery (sham operation, imitating embryo transfer) was performed. Body weight (BW), systolic (SAP) and diastolic (DAP) arterial pressure as well as behavior in open field (OF) and elevated plus maze (EPM) tests were studied in the offspring of females exposed to surgical treatment during pregnancy (OXYSPS) at the age of 3 mo. Untreated offspring of OXYS rats were used as controls. BW in naturally born OXYS rats did not differ from those of the OXYSPS group. OXYS and OXYSPS rats exhibited higher SAP (more than 150 mm Hg) and DAP; it is noteworthy that both SAP and DAP were higher in the OXYSPS group than in the control group. The time spent in the center of arena, the area studied, the time and number of rearing were decreased in OXYSPS rats in the OF test as compared to the OXYS controls. Moreover, OXYSPS rats were characterized by the absence of grooming in the OF test. As was demonstrated by the EPM test, the duration and numbers of peeking out from closed arms were decreased in the OXYSPS rats as compared to the OXYS controls. Thus, OXYS dams’ exposure to surgical stress at their early pregnancy led to such effects in the offspring as elevated SAP and DAP, decreased overall activity and increased anxiety. |
| first_indexed | 2024-03-07T16:06:44Z |
| format | Article |
| id | doaj.art-0437b10640c54f678383f4d26874e466 |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:08:02Z |
| publishDate | 2018-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-0437b10640c54f678383f4d26874e4662024-04-11T15:30:58ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592018-01-0121893794210.18699/VJ17.316705Long-term effects of maternal exposure to surgical stress at the earliest stage of pregnancy on blood pressure and behavior in offspring of OXYS ratsI. N. Rozhkova0T. N. Igonina1D. S. Ragaeva2O. M. Petrova3E. Yu. Brusentsev4V. A. Naprimerov5S. Ya. Amstislavsky6Institute of Cytology and Genetics SB RAS.Institute of Cytology and Genetics SB RAS.Institute of Cytology and Genetics SB RAS.Institute of Cytology and Genetics SB RAS; Novosibirsk State University.Institute of Cytology and Genetics SB RAS.Institute of Cytology and Genetics SB RAS; Novosibirsk State Agrarian University.Institute of Cytology and Genetics SB RAS.The use of some assisted reproductive technologies, in particular, embryo transfer, may cause various physiological and behavioral changes in the offspring. The purpose of our study was to study the effects of surgery (which is used for embryo transfer) done with pregnant dams on the weight, blood pressure and behavior in the open field and elevated plusmaze tests in adult offspring. Thus, longterm effects on the offspring after maternal exposure to surgical stress given to dams at the 4th day of pregnancy were studied in OXYS rats. OXYS females were mated in estrus with fertile males of the same strain. 96 hours after spermatozoa were found in vaginal smears the surgery (sham operation, imitating embryo transfer) was performed. Body weight (BW), systolic (SAP) and diastolic (DAP) arterial pressure as well as behavior in open field (OF) and elevated plus maze (EPM) tests were studied in the offspring of females exposed to surgical treatment during pregnancy (OXYSPS) at the age of 3 mo. Untreated offspring of OXYS rats were used as controls. BW in naturally born OXYS rats did not differ from those of the OXYSPS group. OXYS and OXYSPS rats exhibited higher SAP (more than 150 mm Hg) and DAP; it is noteworthy that both SAP and DAP were higher in the OXYSPS group than in the control group. The time spent in the center of arena, the area studied, the time and number of rearing were decreased in OXYSPS rats in the OF test as compared to the OXYS controls. Moreover, OXYSPS rats were characterized by the absence of grooming in the OF test. As was demonstrated by the EPM test, the duration and numbers of peeking out from closed arms were decreased in the OXYSPS rats as compared to the OXYS controls. Thus, OXYS dams’ exposure to surgical stress at their early pregnancy led to such effects in the offspring as elevated SAP and DAP, decreased overall activity and increased anxiety.https://vavilov.elpub.ru/jour/article/view/1271oxys ratspregnancysurgical treatmentlongterm effects |
| spellingShingle | I. N. Rozhkova T. N. Igonina D. S. Ragaeva O. M. Petrova E. Yu. Brusentsev V. A. Naprimerov S. Ya. Amstislavsky Long-term effects of maternal exposure to surgical stress at the earliest stage of pregnancy on blood pressure and behavior in offspring of OXYS rats Вавиловский журнал генетики и селекции oxys rats pregnancy surgical treatment longterm effects |
| title | Long-term effects of maternal exposure to surgical stress at the earliest stage of pregnancy on blood pressure and behavior in offspring of OXYS rats |
| title_full | Long-term effects of maternal exposure to surgical stress at the earliest stage of pregnancy on blood pressure and behavior in offspring of OXYS rats |
| title_fullStr | Long-term effects of maternal exposure to surgical stress at the earliest stage of pregnancy on blood pressure and behavior in offspring of OXYS rats |
| title_full_unstemmed | Long-term effects of maternal exposure to surgical stress at the earliest stage of pregnancy on blood pressure and behavior in offspring of OXYS rats |
| title_short | Long-term effects of maternal exposure to surgical stress at the earliest stage of pregnancy on blood pressure and behavior in offspring of OXYS rats |
| title_sort | long term effects of maternal exposure to surgical stress at the earliest stage of pregnancy on blood pressure and behavior in offspring of oxys rats |
| topic | oxys rats pregnancy surgical treatment longterm effects |
| url | https://vavilov.elpub.ru/jour/article/view/1271 |
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