A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
Polytene chromosome preparations were obtained from larval, pupal and adult female Malpighian tubules of Aedes aegypti. The Malpighian tubules of the pupae (0-4 h old) from larvae reared at 20ºC provided the best cytogenetic analysis. The interaction of nucleic acids and proteins that influence the...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2003-04-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300017 |
| _version_ | 1797718128475504640 |
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| author | Jairo Campos Carlos Fernando S Andrade Shirlei M Recco-Pimentel |
| author_facet | Jairo Campos Carlos Fernando S Andrade Shirlei M Recco-Pimentel |
| author_sort | Jairo Campos |
| collection | DOAJ |
| description | Polytene chromosome preparations were obtained from larval, pupal and adult female Malpighian tubules of Aedes aegypti. The Malpighian tubules of the pupae (0-4 h old) from larvae reared at 20ºC provided the best cytogenetic analysis. The interaction of nucleic acids and proteins that influence the spreading of the chromosomes could be reduced with the preparation technique of the sheets submitted to a stronger treatment starting with the hypotony of tissue and successive bathings with acetic acid. A simple technique should facilitate molecular cytogenetics used in the location of resistance and vector competence genes. |
| first_indexed | 2024-03-12T08:46:25Z |
| format | Article |
| id | doaj.art-043969874cb94521ba7074799c1af810 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:46:25Z |
| publishDate | 2003-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-043969874cb94521ba7074799c1af8102023-09-02T16:26:07ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602003-04-0198338739010.1590/S0074-02762003000300017A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)Jairo CamposCarlos Fernando S AndradeShirlei M Recco-PimentelPolytene chromosome preparations were obtained from larval, pupal and adult female Malpighian tubules of Aedes aegypti. The Malpighian tubules of the pupae (0-4 h old) from larvae reared at 20ºC provided the best cytogenetic analysis. The interaction of nucleic acids and proteins that influence the spreading of the chromosomes could be reduced with the preparation technique of the sheets submitted to a stronger treatment starting with the hypotony of tissue and successive bathings with acetic acid. A simple technique should facilitate molecular cytogenetics used in the location of resistance and vector competence genes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300017cytologymosquitovector |
| spellingShingle | Jairo Campos Carlos Fernando S Andrade Shirlei M Recco-Pimentel A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae) Memorias do Instituto Oswaldo Cruz cytology mosquito vector |
| title | A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae) |
| title_full | A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae) |
| title_fullStr | A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae) |
| title_full_unstemmed | A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae) |
| title_short | A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae) |
| title_sort | technique for preparing polytene chromosomes from aedes aegypti diptera culicinae |
| topic | cytology mosquito vector |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300017 |
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