A Marine Sulfate-Reducing Bacterium Producing Multiple Antibiotics: Biological and Chemical Investigation
A marine sulfate-reducing bacterium SRB-22 was isolated by means of the agar shake dilution method and identified as Desulfovibrio desulfuricans by morphological, physiological and biochemical characteristics and 16S rDNA analysis. In the bioassay, its extract showed broad-spectrum antimicrobial act...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2009-07-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/7/3/341/ |
| _version_ | 1798024578441674752 |
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| author | Xiaoliang Wang Chenyan Zhao Xiaojie Gu Jun Mu Zeping Xie Yi Zhang |
| author_facet | Xiaoliang Wang Chenyan Zhao Xiaojie Gu Jun Mu Zeping Xie Yi Zhang |
| author_sort | Xiaoliang Wang |
| collection | DOAJ |
| description | A marine sulfate-reducing bacterium SRB-22 was isolated by means of the agar shake dilution method and identified as Desulfovibrio desulfuricans by morphological, physiological and biochemical characteristics and 16S rDNA analysis. In the bioassay, its extract showed broad-spectrum antimicrobial activity using the paper disc agar diffusion method. This isolate showed a different antimicrobial profile than either ampicillin or nystatin and was found to produce at least eight antimicrobial components by bioautography. Suitable fermentation conditions for production of the active constituents were determined to be 28 day cultivation at 25 °C to 30 °C with a 10% inoculation ratio. Under these conditions, the SRB-22 was fermented, extracted and chemically investigated. So far an antimicrobial compound, mono-n-butyl phthalate, and an inactive compound, thymine, have been isolated and characterized. |
| first_indexed | 2024-04-11T18:05:59Z |
| format | Article |
| id | doaj.art-0439c2f9aa2e4cf5a1a52eebc7e0d506 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T18:05:59Z |
| publishDate | 2009-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-0439c2f9aa2e4cf5a1a52eebc7e0d5062022-12-22T04:10:20ZengMDPI AGMarine Drugs1660-33972009-07-017334135410.3390/md7030341A Marine Sulfate-Reducing Bacterium Producing Multiple Antibiotics: Biological and Chemical InvestigationXiaoliang WangChenyan ZhaoXiaojie GuJun MuZeping XieYi ZhangA marine sulfate-reducing bacterium SRB-22 was isolated by means of the agar shake dilution method and identified as Desulfovibrio desulfuricans by morphological, physiological and biochemical characteristics and 16S rDNA analysis. In the bioassay, its extract showed broad-spectrum antimicrobial activity using the paper disc agar diffusion method. This isolate showed a different antimicrobial profile than either ampicillin or nystatin and was found to produce at least eight antimicrobial components by bioautography. Suitable fermentation conditions for production of the active constituents were determined to be 28 day cultivation at 25 °C to 30 °C with a 10% inoculation ratio. Under these conditions, the SRB-22 was fermented, extracted and chemically investigated. So far an antimicrobial compound, mono-n-butyl phthalate, and an inactive compound, thymine, have been isolated and characterized.http://www.mdpi.com/1660-3397/7/3/341/marine sulfate-reducing bacteriumisolationidentificationbroad-spectrum antimicrobial activitymono-n-butyl phthalate |
| spellingShingle | Xiaoliang Wang Chenyan Zhao Xiaojie Gu Jun Mu Zeping Xie Yi Zhang A Marine Sulfate-Reducing Bacterium Producing Multiple Antibiotics: Biological and Chemical Investigation Marine Drugs marine sulfate-reducing bacterium isolation identification broad-spectrum antimicrobial activity mono-n-butyl phthalate |
| title | A Marine Sulfate-Reducing Bacterium Producing Multiple Antibiotics: Biological and Chemical Investigation |
| title_full | A Marine Sulfate-Reducing Bacterium Producing Multiple Antibiotics: Biological and Chemical Investigation |
| title_fullStr | A Marine Sulfate-Reducing Bacterium Producing Multiple Antibiotics: Biological and Chemical Investigation |
| title_full_unstemmed | A Marine Sulfate-Reducing Bacterium Producing Multiple Antibiotics: Biological and Chemical Investigation |
| title_short | A Marine Sulfate-Reducing Bacterium Producing Multiple Antibiotics: Biological and Chemical Investigation |
| title_sort | marine sulfate reducing bacterium producing multiple antibiotics biological and chemical investigation |
| topic | marine sulfate-reducing bacterium isolation identification broad-spectrum antimicrobial activity mono-n-butyl phthalate |
| url | http://www.mdpi.com/1660-3397/7/3/341/ |
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