Genome-Wide Identification and Transcriptional Expression Analysis of Annexin Genes in <i>Capsicum annuum</i> and Characterization of <i>CaAnn9</i> in Salt Tolerance
Annexin (Ann) is a polygenic, evolutionarily conserved, calcium-dependent and phospholipid-binding protein family, which plays key roles in plant growth, development, and stress response. However, a comprehensive understanding of <i>CaAnn</i> genes of pepper (<i>Capsicum annuum<...
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/16/8667 |
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| author | Xiaoxia Wu Yan Ren Hailong Jiang Yan Wang Jiaxing Yan Xiaoying Xu Fucai Zhou Haidong Ding |
| author_facet | Xiaoxia Wu Yan Ren Hailong Jiang Yan Wang Jiaxing Yan Xiaoying Xu Fucai Zhou Haidong Ding |
| author_sort | Xiaoxia Wu |
| collection | DOAJ |
| description | Annexin (Ann) is a polygenic, evolutionarily conserved, calcium-dependent and phospholipid-binding protein family, which plays key roles in plant growth, development, and stress response. However, a comprehensive understanding of <i>CaAnn</i> genes of pepper (<i>Capsicum annuum</i>) at the genome-wide level is limited. Based on the available pepper genomic information, we identified 15 members of the <i>CaAnn</i> gene family. Phylogenetic analysis showed that CaAnn proteins could be categorized into four different orthologous groups. Real time quantitative RT-PCR analysis showed that the <i>CaAnn</i> genes were tissue-specific and were widely expressed in pepper leaves after treatments with cold, salt, and drought, as well as exogenously applied MeJA and ABA. In addition, the function of <i>CaAnn9</i> was further explored using the virus-induced gene silencing (VIGS) technique. <i>CaAnn9</i>-silenced pepper seedlings were more sensitive to salt stress, reflected by the degradation of chlorophyll, the accumulation of reactive oxygen species (ROS), and the decrease of antioxidant defense capacity. This study provides important information for further study of the role of pepper <i>CaAnn</i> genes and their coding proteins in growth, development, and environmental responses. |
| first_indexed | 2024-03-10T08:44:29Z |
| format | Article |
| id | doaj.art-043cdd3fb58f4a6da94a3a5ecc74b662 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:44:29Z |
| publishDate | 2021-08-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-043cdd3fb58f4a6da94a3a5ecc74b6622023-11-22T07:58:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216866710.3390/ijms22168667Genome-Wide Identification and Transcriptional Expression Analysis of Annexin Genes in <i>Capsicum annuum</i> and Characterization of <i>CaAnn9</i> in Salt ToleranceXiaoxia Wu0Yan Ren1Hailong Jiang2Yan Wang3Jiaxing Yan4Xiaoying Xu5Fucai Zhou6Haidong Ding7Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaAnnexin (Ann) is a polygenic, evolutionarily conserved, calcium-dependent and phospholipid-binding protein family, which plays key roles in plant growth, development, and stress response. However, a comprehensive understanding of <i>CaAnn</i> genes of pepper (<i>Capsicum annuum</i>) at the genome-wide level is limited. Based on the available pepper genomic information, we identified 15 members of the <i>CaAnn</i> gene family. Phylogenetic analysis showed that CaAnn proteins could be categorized into four different orthologous groups. Real time quantitative RT-PCR analysis showed that the <i>CaAnn</i> genes were tissue-specific and were widely expressed in pepper leaves after treatments with cold, salt, and drought, as well as exogenously applied MeJA and ABA. In addition, the function of <i>CaAnn9</i> was further explored using the virus-induced gene silencing (VIGS) technique. <i>CaAnn9</i>-silenced pepper seedlings were more sensitive to salt stress, reflected by the degradation of chlorophyll, the accumulation of reactive oxygen species (ROS), and the decrease of antioxidant defense capacity. This study provides important information for further study of the role of pepper <i>CaAnn</i> genes and their coding proteins in growth, development, and environmental responses.https://www.mdpi.com/1422-0067/22/16/8667annexinbioinformatic analysis<i>CaAnn9</i>oxidative damagepeppersalt stress |
| spellingShingle | Xiaoxia Wu Yan Ren Hailong Jiang Yan Wang Jiaxing Yan Xiaoying Xu Fucai Zhou Haidong Ding Genome-Wide Identification and Transcriptional Expression Analysis of Annexin Genes in <i>Capsicum annuum</i> and Characterization of <i>CaAnn9</i> in Salt Tolerance International Journal of Molecular Sciences annexin bioinformatic analysis <i>CaAnn9</i> oxidative damage pepper salt stress |
| title | Genome-Wide Identification and Transcriptional Expression Analysis of Annexin Genes in <i>Capsicum annuum</i> and Characterization of <i>CaAnn9</i> in Salt Tolerance |
| title_full | Genome-Wide Identification and Transcriptional Expression Analysis of Annexin Genes in <i>Capsicum annuum</i> and Characterization of <i>CaAnn9</i> in Salt Tolerance |
| title_fullStr | Genome-Wide Identification and Transcriptional Expression Analysis of Annexin Genes in <i>Capsicum annuum</i> and Characterization of <i>CaAnn9</i> in Salt Tolerance |
| title_full_unstemmed | Genome-Wide Identification and Transcriptional Expression Analysis of Annexin Genes in <i>Capsicum annuum</i> and Characterization of <i>CaAnn9</i> in Salt Tolerance |
| title_short | Genome-Wide Identification and Transcriptional Expression Analysis of Annexin Genes in <i>Capsicum annuum</i> and Characterization of <i>CaAnn9</i> in Salt Tolerance |
| title_sort | genome wide identification and transcriptional expression analysis of annexin genes in i capsicum annuum i and characterization of i caann9 i in salt tolerance |
| topic | annexin bioinformatic analysis <i>CaAnn9</i> oxidative damage pepper salt stress |
| url | https://www.mdpi.com/1422-0067/22/16/8667 |
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