Defense genes expressed by chemical elicitors and Rhizoctonia solani infection in mungbean (Vigna radiata)
The effects of chemical elicitors salicylic acid (SA) and jasmonic acid (JA) on expression of defense genes PR 10, epoxide hydrolase (EH), catalase and calmodulin alone and with infection by Rhizoctonia solani were analysed using qPCR at 1–4 days post inoculation/application (dpi) in highly suscept...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2023-01-01
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| Series: | The Indian Journal of Agricultural Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/106363 |
| _version_ | 1811176058439860224 |
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| author | ARADHIKA TRIPATHI SUNIL C DUBEY RAKESH TAK |
| author_facet | ARADHIKA TRIPATHI SUNIL C DUBEY RAKESH TAK |
| author_sort | ARADHIKA TRIPATHI |
| collection | DOAJ |
| description |
The effects of chemical elicitors salicylic acid (SA) and jasmonic acid (JA) on expression of defense genes PR 10, epoxide hydrolase (EH), catalase and calmodulin alone and with infection by Rhizoctonia solani were analysed using qPCR at 1–4 days post inoculation/application (dpi) in highly susceptible (HS; Ratna) and moderately resistant (MR; HUM1) varieties of mungbean [Vigna radiata (L.) Wilczek] during 2015 and 2016 at Indian Agricultural Research Institute, New Delhi and National Bureau of Plant Genetic Resources, New Delhi. In general, JA application in presence of R. solani upregulated the mean expression of these genes in both the varieties ranging from 1.8–11.9- fold, whereas SA application in presence of R. solani upregulated the mean expression from 1.2–3.4-fold. The JA and SA alone, also upregulated the expression of these genes in both HS and MR varieties with higher expression in MR variety. The expression of calmodulin and PR10 was higher at 1 dpi in HS variety while EH and catalase was higher at 4 dpi. In MR variety, PR10 was higher at 1 dpi and EH, catalase and calmodulin were over expressed at 4 dpi. The results clearly indicated that both JA and SA were responsible for upregulation of the defense genes in both HS and MR varieties of mungbean. The genes expression was accelerated in presence of R. solani at early stage of plant growth which delayed the infection caused by R. solani and helped to reduce disease development, hence may be used for the management of the disease.
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| first_indexed | 2024-04-10T19:45:53Z |
| format | Article |
| id | doaj.art-043f677fea0a49e9b52f2f890c353a16 |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-04-10T19:45:53Z |
| publishDate | 2023-01-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-043f677fea0a49e9b52f2f890c353a162023-01-29T04:34:21ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192023-01-0193110.56093/ijas.v93i1.106363Defense genes expressed by chemical elicitors and Rhizoctonia solani infection in mungbean (Vigna radiata)ARADHIKA TRIPATHI0SUNIL C DUBEY1RAKESH TAK2ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, IndiaIndian Council of Agricultural Research, New DelhiICAR-Indian Agricultural Research Institute, New Delhi The effects of chemical elicitors salicylic acid (SA) and jasmonic acid (JA) on expression of defense genes PR 10, epoxide hydrolase (EH), catalase and calmodulin alone and with infection by Rhizoctonia solani were analysed using qPCR at 1–4 days post inoculation/application (dpi) in highly susceptible (HS; Ratna) and moderately resistant (MR; HUM1) varieties of mungbean [Vigna radiata (L.) Wilczek] during 2015 and 2016 at Indian Agricultural Research Institute, New Delhi and National Bureau of Plant Genetic Resources, New Delhi. In general, JA application in presence of R. solani upregulated the mean expression of these genes in both the varieties ranging from 1.8–11.9- fold, whereas SA application in presence of R. solani upregulated the mean expression from 1.2–3.4-fold. The JA and SA alone, also upregulated the expression of these genes in both HS and MR varieties with higher expression in MR variety. The expression of calmodulin and PR10 was higher at 1 dpi in HS variety while EH and catalase was higher at 4 dpi. In MR variety, PR10 was higher at 1 dpi and EH, catalase and calmodulin were over expressed at 4 dpi. The results clearly indicated that both JA and SA were responsible for upregulation of the defense genes in both HS and MR varieties of mungbean. The genes expression was accelerated in presence of R. solani at early stage of plant growth which delayed the infection caused by R. solani and helped to reduce disease development, hence may be used for the management of the disease. https://epubs.icar.org.in/index.php/IJAgS/article/view/106363Defense genesInduced resistanceMethyl jasmonateSalicylic acid |
| spellingShingle | ARADHIKA TRIPATHI SUNIL C DUBEY RAKESH TAK Defense genes expressed by chemical elicitors and Rhizoctonia solani infection in mungbean (Vigna radiata) The Indian Journal of Agricultural Sciences Defense genes Induced resistance Methyl jasmonate Salicylic acid |
| title | Defense genes expressed by chemical elicitors and Rhizoctonia solani infection in mungbean (Vigna radiata) |
| title_full | Defense genes expressed by chemical elicitors and Rhizoctonia solani infection in mungbean (Vigna radiata) |
| title_fullStr | Defense genes expressed by chemical elicitors and Rhizoctonia solani infection in mungbean (Vigna radiata) |
| title_full_unstemmed | Defense genes expressed by chemical elicitors and Rhizoctonia solani infection in mungbean (Vigna radiata) |
| title_short | Defense genes expressed by chemical elicitors and Rhizoctonia solani infection in mungbean (Vigna radiata) |
| title_sort | defense genes expressed by chemical elicitors and rhizoctonia solani infection in mungbean vigna radiata |
| topic | Defense genes Induced resistance Methyl jasmonate Salicylic acid |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/106363 |
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