Corydalis saxicola Bunting Total Alkaloids ameliorate diet-induced non-alcoholic steatohepatitis by regulating hepatic PI3K/Akt and TLR4/NF-κB pathways in mice
Corydalis saxicola Bunting (Yanhuanglian), distributed in Southwest China, is mainly used for treatment of hepatitis, oral mucosal erosion, conjunctivitis, dysentery, acute abdominal pain and hemorrhoids in the folk. Corydalis saxicola Bunting Total Alkaloids (CSBTA) are the active ingredients extra...
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| Language: | English |
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Elsevier
2022-07-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332222005212 |
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| author | Jiejie Wu Ping Chen Linjie Ju Renhao Gao Silu Li Ziqian Huang Yiqiu Cheng Shuqi Gui Zhixia Qiu Jun Cheng Fang Huang |
| author_facet | Jiejie Wu Ping Chen Linjie Ju Renhao Gao Silu Li Ziqian Huang Yiqiu Cheng Shuqi Gui Zhixia Qiu Jun Cheng Fang Huang |
| author_sort | Jiejie Wu |
| collection | DOAJ |
| description | Corydalis saxicola Bunting (Yanhuanglian), distributed in Southwest China, is mainly used for treatment of hepatitis, oral mucosal erosion, conjunctivitis, dysentery, acute abdominal pain and hemorrhoids in the folk. Corydalis saxicola Bunting Total Alkaloids (CSBTA) are the active ingredients extracted from the root of C. saxicola bunting. Non-alcoholic steatohepatitis (NASH) is the hinge between steatosis and cirrhosis in the spectrum of Non-alcoholic fatty liver disease (NAFLD), which has become one of the most common chronic liver diseases in the world. CSBTA can reduce tumors and brain diseases through anti-inflammatory and antioxidant pathways. Our study was designed to clarify the effects of CSBTA on the HFHC (High fat and high carbohydrate drinking) diet induced mice. In our research, A HFHC diet induced NASH mice model was applied to investigate the effects of CSBTA in vivo and obeticholic acid (OA) was set as positive control. Moreover, the underlying mechanisms were explored by palmitic acid (PA) and lipopolysaccharide (LPS) stimulated HepG2 cells in vitro. The in vivo study illustrated that CSBTA could alleviate mice away from the onset of NASH, and reduce intrahepatocellular lipid accumulation and hepatocyte inflammation under high fat condition. Further in vitro analysis confirmed that CSBTA attenuated inflammation and hepatic lipid accumulation by improving hepatic PI3K/Akt and suppressing hepatic TLR4/NF-κB pathways. In summary, this study demonstrated that CSBTA might be a promising compound for the treatment of NAFLD. |
| first_indexed | 2024-04-13T17:42:04Z |
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| id | doaj.art-04426013a34d41b69710896c61edd8df |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-13T17:42:04Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-04426013a34d41b69710896c61edd8df2022-12-22T02:37:09ZengElsevierBiomedicine & Pharmacotherapy0753-33222022-07-01151113132Corydalis saxicola Bunting Total Alkaloids ameliorate diet-induced non-alcoholic steatohepatitis by regulating hepatic PI3K/Akt and TLR4/NF-κB pathways in miceJiejie Wu0Ping Chen1Linjie Ju2Renhao Gao3Silu Li4Ziqian Huang5Yiqiu Cheng6Shuqi Gui7Zhixia Qiu8Jun Cheng9Fang Huang10Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian avenue, Nanjing, PR ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian avenue, Nanjing, PR ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian avenue, Nanjing, PR ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian avenue, Nanjing, PR ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian avenue, Nanjing, PR ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian avenue, Nanjing, PR ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian avenue, Nanjing, PR ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian avenue, Nanjing, PR China; Nanjing Zhongshan Pharmaceutical Co, Ltd., 21 Hengfa Road, Nanjing Economic and Technological Development Zone, Nanjing, PR ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian avenue, Nanjing, PR ChinaNanjing Zhongshan Pharmaceutical Co, Ltd., 21 Hengfa Road, Nanjing Economic and Technological Development Zone, Nanjing, PR ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian avenue, Nanjing, PR China; Corresponding author.Corydalis saxicola Bunting (Yanhuanglian), distributed in Southwest China, is mainly used for treatment of hepatitis, oral mucosal erosion, conjunctivitis, dysentery, acute abdominal pain and hemorrhoids in the folk. Corydalis saxicola Bunting Total Alkaloids (CSBTA) are the active ingredients extracted from the root of C. saxicola bunting. Non-alcoholic steatohepatitis (NASH) is the hinge between steatosis and cirrhosis in the spectrum of Non-alcoholic fatty liver disease (NAFLD), which has become one of the most common chronic liver diseases in the world. CSBTA can reduce tumors and brain diseases through anti-inflammatory and antioxidant pathways. Our study was designed to clarify the effects of CSBTA on the HFHC (High fat and high carbohydrate drinking) diet induced mice. In our research, A HFHC diet induced NASH mice model was applied to investigate the effects of CSBTA in vivo and obeticholic acid (OA) was set as positive control. Moreover, the underlying mechanisms were explored by palmitic acid (PA) and lipopolysaccharide (LPS) stimulated HepG2 cells in vitro. The in vivo study illustrated that CSBTA could alleviate mice away from the onset of NASH, and reduce intrahepatocellular lipid accumulation and hepatocyte inflammation under high fat condition. Further in vitro analysis confirmed that CSBTA attenuated inflammation and hepatic lipid accumulation by improving hepatic PI3K/Akt and suppressing hepatic TLR4/NF-κB pathways. In summary, this study demonstrated that CSBTA might be a promising compound for the treatment of NAFLD.http://www.sciencedirect.com/science/article/pii/S0753332222005212Corydalis saxicola Bunting Total AlkaloidsNon-alcoholic steatohepatitisNon-alcoholic fatty liver diseasePI3K/Akt and TLR4/NF-κB pathways |
| spellingShingle | Jiejie Wu Ping Chen Linjie Ju Renhao Gao Silu Li Ziqian Huang Yiqiu Cheng Shuqi Gui Zhixia Qiu Jun Cheng Fang Huang Corydalis saxicola Bunting Total Alkaloids ameliorate diet-induced non-alcoholic steatohepatitis by regulating hepatic PI3K/Akt and TLR4/NF-κB pathways in mice Biomedicine & Pharmacotherapy Corydalis saxicola Bunting Total Alkaloids Non-alcoholic steatohepatitis Non-alcoholic fatty liver disease PI3K/Akt and TLR4/NF-κB pathways |
| title | Corydalis saxicola Bunting Total Alkaloids ameliorate diet-induced non-alcoholic steatohepatitis by regulating hepatic PI3K/Akt and TLR4/NF-κB pathways in mice |
| title_full | Corydalis saxicola Bunting Total Alkaloids ameliorate diet-induced non-alcoholic steatohepatitis by regulating hepatic PI3K/Akt and TLR4/NF-κB pathways in mice |
| title_fullStr | Corydalis saxicola Bunting Total Alkaloids ameliorate diet-induced non-alcoholic steatohepatitis by regulating hepatic PI3K/Akt and TLR4/NF-κB pathways in mice |
| title_full_unstemmed | Corydalis saxicola Bunting Total Alkaloids ameliorate diet-induced non-alcoholic steatohepatitis by regulating hepatic PI3K/Akt and TLR4/NF-κB pathways in mice |
| title_short | Corydalis saxicola Bunting Total Alkaloids ameliorate diet-induced non-alcoholic steatohepatitis by regulating hepatic PI3K/Akt and TLR4/NF-κB pathways in mice |
| title_sort | corydalis saxicola bunting total alkaloids ameliorate diet induced non alcoholic steatohepatitis by regulating hepatic pi3k akt and tlr4 nf κb pathways in mice |
| topic | Corydalis saxicola Bunting Total Alkaloids Non-alcoholic steatohepatitis Non-alcoholic fatty liver disease PI3K/Akt and TLR4/NF-κB pathways |
| url | http://www.sciencedirect.com/science/article/pii/S0753332222005212 |
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