Biosynthesis of Akaeolide and Lorneic Acids and Annotation of Type I Polyketide Synthase Gene Clusters in the Genome of Streptomyces sp. NPS554
The incorporation pattern of biosynthetic precursors into two structurally unique polyketides, akaeolide and lorneic acid A, was elucidated by feeding experiments with 13C-labeled precursors. In addition, the draft genome sequence of the producer, Streptomyces sp. NPS554, was performed and the biosy...
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| Format: | Article |
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MDPI AG
2015-01-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/13/1/581 |
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| author | Tao Zhou Hisayuki Komaki Natsuko Ichikawa Akira Hosoyama Seizo Sato Yasuhiro Igarashi |
| author_facet | Tao Zhou Hisayuki Komaki Natsuko Ichikawa Akira Hosoyama Seizo Sato Yasuhiro Igarashi |
| author_sort | Tao Zhou |
| collection | DOAJ |
| description | The incorporation pattern of biosynthetic precursors into two structurally unique polyketides, akaeolide and lorneic acid A, was elucidated by feeding experiments with 13C-labeled precursors. In addition, the draft genome sequence of the producer, Streptomyces sp. NPS554, was performed and the biosynthetic gene clusters for these polyketides were identified. The putative gene clusters contain all the polyketide synthase (PKS) domains necessary for assembly of the carbon skeletons. Combined with the 13C-labeling results, gene function prediction enabled us to propose biosynthetic pathways involving unusual carbon-carbon bond formation reactions. Genome analysis also indicated the presence of at least ten orphan type I PKS gene clusters that might be responsible for the production of new polyketides. |
| first_indexed | 2024-04-11T22:22:27Z |
| format | Article |
| id | doaj.art-04431ddca7a74fc5b198af2eae089879 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T22:22:27Z |
| publishDate | 2015-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-04431ddca7a74fc5b198af2eae0898792022-12-22T04:00:00ZengMDPI AGMarine Drugs1660-33972015-01-0113158159610.3390/md13010581md13010581Biosynthesis of Akaeolide and Lorneic Acids and Annotation of Type I Polyketide Synthase Gene Clusters in the Genome of Streptomyces sp. NPS554Tao Zhou0Hisayuki Komaki1Natsuko Ichikawa2Akira Hosoyama3Seizo Sato4Yasuhiro Igarashi5Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, JapanBiological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusa-kamatari, Kisarazu, Chiba 292-0818, JapanBiological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-4-19 Nishihara, Shibuya-ku, Tokyo 151-0066, JapanBiological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-4-19 Nishihara, Shibuya-ku, Tokyo 151-0066, JapanCentral Research Laboratory, Nippon Suisan Kaisha, Ltd., Tokyo Innovation Center, 1-32-3 Nanakuni, Hachioji, Tokyo 192-0991, JapanBiotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, JapanThe incorporation pattern of biosynthetic precursors into two structurally unique polyketides, akaeolide and lorneic acid A, was elucidated by feeding experiments with 13C-labeled precursors. In addition, the draft genome sequence of the producer, Streptomyces sp. NPS554, was performed and the biosynthetic gene clusters for these polyketides were identified. The putative gene clusters contain all the polyketide synthase (PKS) domains necessary for assembly of the carbon skeletons. Combined with the 13C-labeling results, gene function prediction enabled us to propose biosynthetic pathways involving unusual carbon-carbon bond formation reactions. Genome analysis also indicated the presence of at least ten orphan type I PKS gene clusters that might be responsible for the production of new polyketides.http://www.mdpi.com/1660-3397/13/1/581polyketidemarine actinomyceteStreptomycesbiosynthesisgenome |
| spellingShingle | Tao Zhou Hisayuki Komaki Natsuko Ichikawa Akira Hosoyama Seizo Sato Yasuhiro Igarashi Biosynthesis of Akaeolide and Lorneic Acids and Annotation of Type I Polyketide Synthase Gene Clusters in the Genome of Streptomyces sp. NPS554 Marine Drugs polyketide marine actinomycete Streptomyces biosynthesis genome |
| title | Biosynthesis of Akaeolide and Lorneic Acids and Annotation of Type I Polyketide Synthase Gene Clusters in the Genome of Streptomyces sp. NPS554 |
| title_full | Biosynthesis of Akaeolide and Lorneic Acids and Annotation of Type I Polyketide Synthase Gene Clusters in the Genome of Streptomyces sp. NPS554 |
| title_fullStr | Biosynthesis of Akaeolide and Lorneic Acids and Annotation of Type I Polyketide Synthase Gene Clusters in the Genome of Streptomyces sp. NPS554 |
| title_full_unstemmed | Biosynthesis of Akaeolide and Lorneic Acids and Annotation of Type I Polyketide Synthase Gene Clusters in the Genome of Streptomyces sp. NPS554 |
| title_short | Biosynthesis of Akaeolide and Lorneic Acids and Annotation of Type I Polyketide Synthase Gene Clusters in the Genome of Streptomyces sp. NPS554 |
| title_sort | biosynthesis of akaeolide and lorneic acids and annotation of type i polyketide synthase gene clusters in the genome of streptomyces sp nps554 |
| topic | polyketide marine actinomycete Streptomyces biosynthesis genome |
| url | http://www.mdpi.com/1660-3397/13/1/581 |
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