Voluntary exercise preserves visual function and reduces inflammatory response in an adult mouse model of autosomal dominant retinitis pigmentosa
Abstract Whole-body physical exercise has been shown to promote retinal structure and function preservation in animal models of retinal degeneration. It is currently unknown how exercise modulates retinal inflammatory responses. In this study, we investigated cytokine alterations associated with ret...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-57027-9 |
| _version_ | 1797247243163533312 |
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| author | Katie L. Bales Austin M. Karesh Kelleigh Hogan Alicia S. Chacko GianMarco L. Douglas Andrew J. Feola John M. Nickerson Alyssa Pybus Levi Wood Jeffrey H. Boatright Machelle T. Pardue |
| author_facet | Katie L. Bales Austin M. Karesh Kelleigh Hogan Alicia S. Chacko GianMarco L. Douglas Andrew J. Feola John M. Nickerson Alyssa Pybus Levi Wood Jeffrey H. Boatright Machelle T. Pardue |
| author_sort | Katie L. Bales |
| collection | DOAJ |
| description | Abstract Whole-body physical exercise has been shown to promote retinal structure and function preservation in animal models of retinal degeneration. It is currently unknown how exercise modulates retinal inflammatory responses. In this study, we investigated cytokine alterations associated with retinal neuroprotection induced by voluntary running wheel exercise in a retinal degeneration mouse model of class B1 autosomal dominant retinitis pigmentosa, I307N Rho. I307N Rho mice undergo rod photoreceptor degeneration when exposed to bright light (induced). Our data show, active induced mice exhibited significant preservation of retinal and visual function compared to inactive induced mice after 4 weeks of exercise. Retinal cytokine expression revealed significant reductions of proinflammatory chemokines, keratinocyte-derived chemokine (KC) and interferon gamma inducible protein-10 (IP-10) expression in active groups compared to inactive groups. Through immunofluorescence, we found KC and IP-10 labeling localized to retinal vasculature marker, collagen IV. These data show that whole-body exercise lowers specific retinal cytokine expression associated with retinal vasculature. Future studies should determine whether suppression of inflammatory responses is requisite for exercise-induced retinal protection. |
| first_indexed | 2024-04-24T19:55:36Z |
| format | Article |
| id | doaj.art-04470ad063504c1684a38351bf46e131 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T19:55:36Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-04470ad063504c1684a38351bf46e1312024-03-24T12:20:30ZengNature PortfolioScientific Reports2045-23222024-03-0114111210.1038/s41598-024-57027-9Voluntary exercise preserves visual function and reduces inflammatory response in an adult mouse model of autosomal dominant retinitis pigmentosaKatie L. Bales0Austin M. Karesh1Kelleigh Hogan2Alicia S. Chacko3GianMarco L. Douglas4Andrew J. Feola5John M. Nickerson6Alyssa Pybus7Levi Wood8Jeffrey H. Boatright9Machelle T. Pardue10Atlanta VA Medical Center for Visual and Neurocognitive RehabilitationWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of TechnologyAtlanta VA Medical Center for Visual and Neurocognitive RehabilitationWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of TechnologyAtlanta VA Medical Center for Visual and Neurocognitive RehabilitationAtlanta VA Medical Center for Visual and Neurocognitive RehabilitationDepartment of Ophthalmology, Emory UniversityWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of TechnologyWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of TechnologyWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of TechnologyAtlanta VA Medical Center for Visual and Neurocognitive RehabilitationAbstract Whole-body physical exercise has been shown to promote retinal structure and function preservation in animal models of retinal degeneration. It is currently unknown how exercise modulates retinal inflammatory responses. In this study, we investigated cytokine alterations associated with retinal neuroprotection induced by voluntary running wheel exercise in a retinal degeneration mouse model of class B1 autosomal dominant retinitis pigmentosa, I307N Rho. I307N Rho mice undergo rod photoreceptor degeneration when exposed to bright light (induced). Our data show, active induced mice exhibited significant preservation of retinal and visual function compared to inactive induced mice after 4 weeks of exercise. Retinal cytokine expression revealed significant reductions of proinflammatory chemokines, keratinocyte-derived chemokine (KC) and interferon gamma inducible protein-10 (IP-10) expression in active groups compared to inactive groups. Through immunofluorescence, we found KC and IP-10 labeling localized to retinal vasculature marker, collagen IV. These data show that whole-body exercise lowers specific retinal cytokine expression associated with retinal vasculature. Future studies should determine whether suppression of inflammatory responses is requisite for exercise-induced retinal protection.https://doi.org/10.1038/s41598-024-57027-9 |
| spellingShingle | Katie L. Bales Austin M. Karesh Kelleigh Hogan Alicia S. Chacko GianMarco L. Douglas Andrew J. Feola John M. Nickerson Alyssa Pybus Levi Wood Jeffrey H. Boatright Machelle T. Pardue Voluntary exercise preserves visual function and reduces inflammatory response in an adult mouse model of autosomal dominant retinitis pigmentosa Scientific Reports |
| title | Voluntary exercise preserves visual function and reduces inflammatory response in an adult mouse model of autosomal dominant retinitis pigmentosa |
| title_full | Voluntary exercise preserves visual function and reduces inflammatory response in an adult mouse model of autosomal dominant retinitis pigmentosa |
| title_fullStr | Voluntary exercise preserves visual function and reduces inflammatory response in an adult mouse model of autosomal dominant retinitis pigmentosa |
| title_full_unstemmed | Voluntary exercise preserves visual function and reduces inflammatory response in an adult mouse model of autosomal dominant retinitis pigmentosa |
| title_short | Voluntary exercise preserves visual function and reduces inflammatory response in an adult mouse model of autosomal dominant retinitis pigmentosa |
| title_sort | voluntary exercise preserves visual function and reduces inflammatory response in an adult mouse model of autosomal dominant retinitis pigmentosa |
| url | https://doi.org/10.1038/s41598-024-57027-9 |
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