Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence

Suspended particles play a significant role in aquatic systems. However, existing methods to probe suspended particles have several limitations. In this paper, we present a portable prototype to in situ probe individual particles in aquatic suspensions by simultaneously measuring polarized light sca...

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Bibliographic Details
Main Authors: Zhihang Xiong, Hongjian Wang, Jiajin Li, Ran Liao, Haoji Mai, Caizhong Guan, Zhiming Guo, Shangpan Yang, Yan Chen, Biwang Liu, Tong Liu, Hongyi Li, Wenzheng Ding, Yaguang Zeng, Hui Ma
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:Biosensors
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Online Access:https://www.mdpi.com/2079-6374/11/11/416
Description
Summary:Suspended particles play a significant role in aquatic systems. However, existing methods to probe suspended particles have several limitations. In this paper, we present a portable prototype to in situ probe individual particles in aquatic suspensions by simultaneously measuring polarized light scattering and fluorescence, aiming to obtain an effective classification of microplastics and microalgae. Results show that the obtained classification accuracy is significantly higher than that for either of these two methods. The setup also successfully measures submicron particles and discriminates two species of <i>Synechococcus</i>. Our study demonstrates the feasibility of simultaneously measuring polarized light scattering and fluorescence, and the promising capability of our method for further aquatic environmental monitoring.
ISSN:2079-6374